A preliminary study for the establishment of a reference interval for vitamin B12 in China after performance verification of a second‐generation ECLIA kit

Jiang, Wencan; Men, Shasha; Wen, Xinyu; Yuan, Xiaozhou; Pu, Danna; Lv, Xiaoting; Jia, Xingwang; Wang, Chengbin

doi:10.1002/jcla.23165

Cited by 5 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12,13 A recent Chinese study reported that a manufacturer's original reference interval from a population of European descent was quite different from the reference interval determined from a local population. 14 A recent study in North India found 47% prevalence of vitamin B12 deficiency using European derived reference interval. 15 This may reflect different study population or indeed different dietary or ethnic attributes.…”

Section: Discussionmentioning

confidence: 99%

Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms

Jassam,

Bancroft,

Luvai

et al. 2023

Ann Clin Biochem

View full text Add to dashboard Cite

Background: Vitamin B12 status is assessed primarily by total serum B12 using competitive binding methods. The lack of availability of a standard material, and high-level reference measurement procedure affects the trueness of B12 results. This results in variation between methods. This study aimed to determine the reference intervals for vitamin B12 on three routine analytical platforms. Method: A prospective reference population of healthy individuals was recruited according to the IFCC CRIDL criteria. Vitamin B12 samples were measured on Roche, Beckman and Siemens analytical platforms. Results: In total, 300 adult subjects were recruited; the central 95th centile values for B12 for Roche (190-678 ng/ml) and Siemens (181-562 ng/ml) analytical platforms were in a close agreement. Beckman DXi however, showed a significantly lower reference limit (110-562 ng/ml). All reference intervals are in keeping with previously published data but some are not in agreement with manufacturer provided reference interval. Conclusion: As the quality of the reference intervals play a significant role in clinical outcome. It is of great importance that laboratories use method-specific reference interval and if possible locally derived reference intervals until further method standardisation occurs.

show abstract

Section: Discussionmentioning

confidence: 99%

Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms

Jassam,

Bancroft,

Luvai

et al. 2023

Ann Clin Biochem

View full text Add to dashboard Cite

show abstract

“…A recent study in China (Jiang et al, 2020) reported that a manufacturer's original RI from a population of European descent was quite different from the RI determined from a Chinese population. In another study, serum concentration of vitamin B12 measured for a large number of individuals with different ethnical backgrounds in London, UK showed ethnic related variation (O'Logbon et al, 2021).…”

Section: Introductionmentioning

confidence: 90%

Extraction of B12 Reference Intervals from a Large Amount of General Patient Data

Gavars¹,

Perminov

Tauckels³

et al. 2022

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

View full text Add to dashboard Cite

This study compared the reference intervals (RI) of B12 vitamin concentration in blood found in the literature with RIs extracted from data accumulated from a large number of patients by E. Gulbis Laboratory in Latvia. This paper investigated and demonstrated the possibility of using large amounts of random patient data to establish the RI for clinical laboratory tests. The blood level of B12 vitamin was selected as the model system for this study. The study used blind data for B12 blood level measurements from 132 379 patients accumulated in E. Gulbis Laboratory over a period of 15 years. In order to establish the reference intervals, the frequency distribution of log transformed B12 values was fit to a Gaussian distribution. The established B12 reference interval of 196 pg/ml and 942 pg/ml was found to be in good agreement with RIs reported elsewhere.

show abstract

“…When there is no catalysis of substrate there is no signal produced. High levels of ruthenium and other substances in multiple sites tend to cause loss of biological activity of molecules 39 .…”

Section: Disadvantagesmentioning

confidence: 99%

Eclia Test- Review

2020

IJFMT

View full text Add to dashboard Cite

Chemiluminescent immunoassay is a modification of standard enzyme immunoassay which is a biochemical technique that has evolved to be used in recent times. Immunoassay is a diagnostic biochemical test used to quantify an analyte or a specific substance from blood or other body fluids. Electrochemiluminescence immunoassay (ECLIA) is a new developing method to determine antibodies in human being's blood sample.this has evolved from various other immunoassay methods that were previously used for detection of enzyme/antibody etc. The test takes place following three principles. Electrochemiluminescence immunoassay testing has been used to detect many markers such as typhoid, cardiac, tumor markers and various infectious diseases in in-vitro analysis. It is also frequently used in the detection of recombinant specific treponemal antigens. Research articles were searched from search engines such as MeSH, pubMed, Core, Google Scholar, Cochrane etc. the time period considere was from 2000 -2020 which is 20 years. Articles were studied and data were collected under different headings. In Spite of many advantages of immunoassays, they have some limitations. Immunoassays rely primarily on activity between analyte and the natural antibody, they may have more intrinsic imprecision than other methods used in medicine analysis. Biological markers might produce false positive results and usage of viral markers is still not found for the process of electrochemiluminescence immunoassay test. Late night salivary cortisol concentration, an indicator of cushing's syndrome, is detected using this test. This research provides in-depth knowledge and awareness of electrochemiluminescence immunoassay tests and paves the way for better diagnostic methods for the future.

show abstract

A preliminary study for the establishment of a reference interval for vitamin B12 in China after performance verification of a second‐generation ECLIA kit

Cited by 5 publications

References 16 publications

Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms

Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms

Extraction of B12 Reference Intervals from a Large Amount of General Patient Data

Eclia Test- Review

Contact Info

Product

Resources

About