Establishment of a direct quantitative method for measurement of microRNA-224 in serum by UHPLC/MS/MS

Wu, Anqi; Ji, Huoyan; Li, Yi; Liu, Ruo-Yu; Hu, Zeyang; Ju, Shaoqing; Wang, Feng

doi:10.1016/j.jchromb.2020.122199

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…Mass spectrometry (MS) has promise as a potent tool to quantificationally measure miRNA in serum because of its high sensitivity, strong specificity and wide dynamic range. 150 Thus, Wang et al developed a quantitative method for miRNA-224 detection by UHPLC/MS/MS, 151 in which the signal of miRNA-224 was converted into the mass response of the reporter peptide. In this method, biotinylated miRNA-224 adhered to the streptavidin agarose, and was recognized by and hybridized with DNA-peptide probes.…”

Section: Methods For Mirna Detectionmentioning

confidence: 99%

Cancer drug resistance related microRNAs: recent advances in detection methods

Song

Yang

et al. 2022

Analyst

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Section: Methods For Mirna Detectionmentioning

confidence: 99%

Cancer drug resistance related microRNAs: recent advances in detection methods

Song

Yang

et al. 2022

Analyst

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“…Furthermore, another study revealed that AFP along with heptoglobin specific N-glycopeptides may achieve a sensitivity of greater than 80% as diagnostic biomarkers for nonalcoholic steatohepatitis HCC patients [102]. Wu and colleagues revealed that triple quadrupole MS (QqQ MS)-based identification of miR-224 in HCC serum samples may also be a potential diagnostic and prognostic marker in clinical practice [103].…”

Section: Hepatocellular Carcinoma (Hcc)mentioning

confidence: 99%

Advances in High Throughput Proteomics Profiling in Establishing Potential Biomarkers for Gastrointestinal Cancer

Khan

Tam

Law

2022

Cells

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Gastrointestinal cancers (GICs) remain the most diagnosed cancers and accounted for the highest cancer-related death globally. The prognosis and treatment outcomes of many GICs are poor because most of the cases are diagnosed in advanced metastatic stages. This is primarily attributed to the deficiency of effective and reliable early diagnostic biomarkers. The existing biomarkers for GICs diagnosis exhibited inadequate specificity and sensitivity. To improve the early diagnosis of GICs, biomarkers with higher specificity and sensitivity are warranted. Proteomics study and its functional analysis focus on elucidating physiological and biological functions of unknown or annotated proteins and deciphering cellular mechanisms at molecular levels. In addition, quantitative analysis of translational proteomics is a promising approach in enhancing the early identification and proper management of GICs. In this review, we focus on the advances in mass spectrometry along with the quantitative and functional analysis of proteomics data that contributes to the establishment of biomarkers for GICs including, colorectal, gastric, hepatocellular, pancreatic, and esophageal cancer. We also discuss the future challenges in the validation of proteomics-based biomarkers for their translation into clinics.

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“…To address this issue, researchers have explored alternative methods to enhance MS detection sensitivity. Xu et al suggested a method utilizing peptides as reporters to improve sensitivity. − Shi et al integrated MS detection with a duplex specific nuclease (DSN)-based signal amplification strategy, allowing target miRNAs to recycle and produce large amounts of complementary DNA fragments . Certain methods employed nucleobases as reporters for miRNA analysis, leveraging rolling circle amplification or DSN-assisted amplification. , Despite these indirect methods using peptides, DNA fragments, and nucleobases compensating for the low detection sensitivity, they were considerably complicated and time-consuming for practical use.…”

Section: Introductionmentioning

confidence: 99%

“…Xu et al suggested a method utilizing peptides as reporters to improve sensitivity. 10 − 15 Shi et al integrated MS detection with a duplex specific nuclease (DSN)-based signal amplification strategy, allowing target miRNAs to recycle and produce large amounts of complementary DNA fragments. 16 Certain methods employed nucleobases as reporters for miRNA analysis, leveraging rolling circle amplification or DSN-assisted amplification.…”

Section: Introductionmentioning

confidence: 99%

LC–MS-Based Direct Quantification of MicroRNAs in Rat Blood

Cho,

Min,

Choi

et al. 2023

ACS Omega

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MicroRNA (miRNA) has recently garnered significant research attention, owing to its potential as a diagnostic biomarker and therapeutic target. Liquid chromatography−mass spectrometry (LC−MS) offers accurate quantification, multiplexing capacity, and high compatibility with various matrices. These advantages establish it as a preferred technique for detecting miRNA in biological samples. In this study, we presented an LC− MS method for directly quantifying seven miRNAs (rno-miR-150, 146a, 21, 155, 223, 181a, and 125a) associated with immune and inflammatory responses in rat whole blood. To ensure miRNA stability in the samples and efficiently purify target analytes, we compared Trizol-and proteinase K-based extraction methods, and the Trizol extraction proved to be superior in terms of analytical sensitivity and convenience. Chromatographic separation was carried out using an oligonucleotide C18 column with a mobile phase composed of N-butyldimethylamine, 1,1,1,3,3,3-hexafluoro-2propanol, and methanol. For MS detection, we performed high-resolution full scan analysis using an orbitrap mass analyzer with negative electrospray ionization. The established method was validated by assessing its selectivity, linearity, limit of quantification, accuracy, precision, recovery, matrix effect, carry-over, and stability. The proposed assay was then applied to simultaneously monitor target miRNAs in lipopolysaccharide-treated rats. Although potentially less sensitive than conventional methods, such as qPCR and microarray, this direct-detection-based LC-MS method can accurately and precisely quantify miRNA. Given these promising results, this method could be effectively deployed in various miRNA-related applications.

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Establishment of a direct quantitative method for measurement of microRNA-224 in serum by UHPLC/MS/MS

Cited by 7 publications

References 35 publications

Cancer drug resistance related microRNAs: recent advances in detection methods

Cancer drug resistance related microRNAs: recent advances in detection methods

Advances in High Throughput Proteomics Profiling in Establishing Potential Biomarkers for Gastrointestinal Cancer

LC–MS-Based Direct Quantification of MicroRNAs in Rat Blood

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