Establishment of a Cell Line with Reticulo-endothelial Characteristics from a Rainbow Trout Spleen Explant

Moritomo, Tadaaki; Anderson, D.P.; Schill, William B.

doi:10.3147/jsfp.25.165

Cited by 18 publications

(6 citation statements)

References 8 publications

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“…6C, 6D). These results are consistent with reports that salmonid monocytes/macrophages are positive for BG and NCAE stains while neutrophils are positively stained with MPO and SBB stains (47, 48). The distribution of CD4-1 + myeloid cells was determined by flow cytometry and revealed that significant percentages of CD4-1 + myeloid cells were found in the myeloid cell population of blood (~14%) and HK (~14.9%), whereas relatively low percentages of these cells were observed in spleen (~6.4%) and thymus (~2.8%) (Fig.…”

Section: Resultssupporting

confidence: 93%

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Takizawa

Magadán

Parra

et al. 2016

The Journal of Immunology

103

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Tetrapods contain a single CD4 co-receptor with four immunoglobulin domains that likely arose from a primordial two-domain ancestor. Notably, teleost fish contain two CD4 genes. Like tetrapod CD4, CD4-1 of rainbow trout includes four immunoglobulin domains while CD4-2 contains only two. Since CD4-2 is reminiscent of the prototypic two-domain CD4 co-receptor, we hypothesized that by characterizing the cell types bearing CD4-1 and CD4-2, we would shed light into the evolution and primordial roles of CD4-bearing cells. Using newly established monoclonal antibodies against CD4-1 and CD4-2, we identified two bona fide CD4+ T-cell populations, a predominant lymphocyte population co-expressing surface CD4-1 and CD4-2 (CD4 DP), and a minor subset expressing only CD4-2 (CD4-2 SP). While both subsets produced equivalent levels of Th1, Th17, and Treg cytokines upon bacterial infection, CD4-2 SP lymphocytes were less proliferative and displayed a more restricted TCRβ repertoire. These data suggest that CD4-2 SP cells represent a functionally distinct population and may embody a vestigial CD4+ T cell subset, the roles of which reflect those of primeval CD4+ T cells. Importantly, we also describe the first CD4+ monocyte/macrophage population in a non-mammalian species. Of all myeloid subsets, we found the CD4+ population to be the most phagocytic, while CD4+ lymphocytes lacked this capacity. This study fills in an important gap in the knowledge of teleost CD4-bearing leukocytes thus revealing critical insights into the evolutionary origins and primordial roles of CD4+ lymphocytes and CD4+ monocyte/macrophages.

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Section: Resultssupporting

confidence: 93%

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Takizawa

Magadán

Parra

et al. 2016

The Journal of Immunology

103

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“…A fibroblastlike line was developed from a primary culture that had been prepared by collagenase digestion and maintained in 10% FBS; an epithelial-like cell line, from the outgrowth of explants in 10% FBS. Previously, a cell line with reticular cell characteristics was developed from fragments of the rainbow trout spleen (Moritomo et al, 1990). Other fish spleen cell lines have been developed from the silver perch (Ellender et al, 1979), sturgeon (Hedrick et al, 1991) and black porgy (Tung et al, 1991).…”

Section: Non-haemopoietic Cell Culturesmentioning

confidence: 99%

Development of long-term rainbow trout spleen cultures that are haemopoietic and produce dendritic cells

Ganassin

Bols

1996

Fish & Shellfish Immunology

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“…Fish spleen cell cultures have been used to develop several cell lines (Ellender et al, 1979;Moritomo et al, 1990;Hedrick et al, 1991;Tung et al, 1991), although little is known about the cell populations and physiology of primary cultures. The techniques for obtaining mammalian splenic cultures using either explants (Globerson & Auerbach, 1966) or cell suspensions (Mishell & Dutton, 1967) and their use for immunostimulation assays, have been adapted for fishes (Miller & Clem, 1984;Anderson et al, 1986Anderson et al, , 1991 although only for short periods and without characterising the distinct cell populations in the cultures.…”

Section: Introductionmentioning

confidence: 99%

Splenic cultures from rainbow trout,Oncorhynchus mykiss: establishment and characterisation

Flaño

López‐Fierro

Álvarez

et al. 1998

Fish & Shellfish Immunology

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Establishment of a Cell Line with Reticulo-endothelial Characteristics from a Rainbow Trout Spleen Explant

Cited by 18 publications

References 8 publications

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Novel Teleost CD4-Bearing Cell Populations Provide Insights into the Evolutionary Origins and Primordial Roles of CD4+ Lymphocytes and CD4+ Macrophages

Development of long-term rainbow trout spleen cultures that are haemopoietic and produce dendritic cells

Splenic cultures from rainbow trout,Oncorhynchus mykiss: establishment and characterisation

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