Development of long-term rainbow trout spleen cultures that are haemopoietic and produce dendritic cells

Ganassin, Rosemarie C.; Bols, Niels C.

doi:10.1006/fsim.1996.0003

Cited by 43 publications

(36 citation statements)

References 30 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These recent reports suggest that fish macrophages may be instrumental in mediating their own proliferation and di#erentiation, via the production of autocrine growth factors. Production of MGF by teleost macrophages may explain why a number of spontaneously arising long-term macrophage cultures have been established from various fish species (Vallejo et al, 1991;Miller et al, 1994;Wang et al, 1995;Ganassin & Bols, 1996;Dannevig et al, 1997;Weyts et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures

Neumann

Barreda

Belosevic

2000

Fish & Shellfish Immunology

132

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures

Neumann

Barreda

Belosevic

2000

Fish & Shellfish Immunology

132

View full text Add to dashboard Cite

“…Several specifi c and innate immune components have been identifi ed and characterized in different fi sh species. The major innate immune components include the mucus layer on the skin, gills and gastrointestinal tract, phagocytes, non-specifi c cytotoxic cells (Evans et al 2001, Neumann et al 2001, as well as epithelial and dendritic cells (Dalmo et al 1996, Ganassin andBols 1996). Among these, fi sh mucus is considered a primary and a key component of fi sh immune system (Ingram 1980).…”

Section: Introductionmentioning

confidence: 99%

Elucidation of innate immune components in the epidermal mucus of different freshwater fish species

Timalata

Krishnaveni

Rao

et al. 2015

Acta Ichthyol. Piscat.

View full text Add to dashboard Cite

., Arockiaraj J. 2015. Elucidation of innate immune components in the epidermal mucus of different freshwater fi sh species. Acta Ichthyol. Piscat. 45 (3): 221-230.Background. Fish epidermal mucus is a key component of innate immune system and plays a major role in protecting fi sh against invading pathogenic microbes. The information on the role of epidermal mucus components is insuffi cient for many commercially important freshwater fi sh species. Hence, this study was directed to understand the innate immune components of epidermal mucus in these fi sh species. Materials and methods. Mucus samples were obtained from African catfi sh, Clarias gariepinus (Burchell, 1822); Indonesian snakehead, Channa micropeltes (Cuvier, 1831); striped snakehead, Channa striatus (Bloch, 1793); marble goby, Oxyeleotris marmorata (Bleeker, 1852); Nile tilapia, Oreochromis niloticus (Linnaeus, 1758); and Asian redtail catfi sh, Hemibagrus nemurus (Valenciennes, 1840). The variation in the protein profi le of the mucus were observed using SDS-PAGE analysis. The specifi c activities of various hydrolytic enzymes including lysozyme, alkaline phosphatase, esterase, and protease were analysed in the mucus and the levels of enzyme activities were compared among the experimental fi shes. We also elucidated the effect of various protease inhibitors on the protease activity and identifi ed the type of proteases in the fi sh mucus by azocasein hydrolysis assay and zymography. Results. Signifi cantly (P < 0.05) highest levels of lysozyme-, alkaline phosphatase-, and esterase activities were observed to be 15.77, 18.96, and 10.65 U · mg -1 protein respectively in C. striatus followed by 13.67, 15.06, and 16.67 U · mg -1 protein, respectively in C. gariepinus. The highest level of protease activity was recorded in H. nemurus (1630.97 U · mg -1 protein) and C. gariepinus (1596.4 U · mg -1 protein). A wide range of variation was noticed in the enzyme activities of fi shes, even from the same genus, for example C. micropeltes and C. striatus. Specifi c inhibitors were added to azocasein hydrolysis assay and zymography experiments to characterize the individual proteases present in the mucus of each fi sh species. This result revealed the highest level of serine proteases in mucus of redtail catfi sh and African catfi sh compared to cysteine and metalloproteases. In contrast, Indonesian snakehead and marble goby had similar levels of serine, cysteine and metalloproteases. Conclusion. This study provides an insight into the presence of various epidermal mucus enzymes in fi shes and therefore believed to be the components of innate immune system that may possibly provide protection for the fi sh against invading pathogenic microbes.

show abstract

“…Various long-term cell cultures and cell lines consisting of haemopoietic precursor cells or stromal cells have been developed from the head kidney and spleen of rainbow trout (Oncorhynchus mykiss) (Diago et al, 1993;Siegl et al, 1993;Diago et al, 1995;Ganassin & Bols, 1996), but these cultures were heterogeneous and leucocytes were not the most prominent cell type.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of a long-term cell line (SHK-1) developed from the head kidney of Atlantic salmon (Salmo salarL.)

Dannevig

Brudeseth

Gjøen

et al. 1997

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Development of long-term rainbow trout spleen cultures that are haemopoietic and produce dendritic cells

Cited by 43 publications

References 30 publications

Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures

Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures

Elucidation of innate immune components in the epidermal mucus of different freshwater fish species

Characterisation of a long-term cell line (SHK-1) developed from the head kidney of Atlantic salmon (Salmo salarL.)

Contact Info

Product

Resources

About