Characterisation of a long-term cell line (SHK-1) developed from the head kidney of Atlantic salmon (Salmo salarL.)

Dannevig, Birgit H.; Brudeseth, Bjørn; Gjøen, Tor; Rode, Marit; Wergeland, Heidrun I.; Evensen, Øystein; Press, Charles McL.

doi:10.1006/fsim.1996.0076

Cited by 81 publications

(56 citation statements)

References 26 publications

(35 reference statements)

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“…To our knowledge, studies concerning the adherence and invasive properties of V. ordalii have not been conducted. In the present study, we chose a cell line derived from leucocytes that have some of the properties of macrophages (Dannevig et al 1997). Our findings on SHK-1 cells showed that regardless of the strain studied, the Atlantic salmon V. ordalii isolates adhered strongly to the cultured fish cells after 2 hpi, while the bacterial count in SHK-1 cells showed a negligible invasion according to the inoculum size at 24 hpi.…”

Section: Discussionmentioning

confidence: 89%

Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

et al. 2015

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Section: Discussionmentioning

confidence: 89%

Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

et al. 2015

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“…In order to study this process in more detail we searched for a cellular model for salmon lipid homeostasis. Due to the scarcity of salmon cell lines and the absence of a hepatocyte-like model, we targeted the macrophage-like SHK-1 cell line [28,29]. This was based on the rationale that the critical transcriptional regulators of macrophage lipid homeostasis are the same as those in liver, and they regulate similar target genes [40].…”

Section: Discussionmentioning

confidence: 99%

“…The Atlantic salmon established cell line, SHK-1, originated from salmon head kidney tissue and are likely leucocyte-derived, showing both macrophage-or dentritic-like phenotype [28,29]. The cells were routinely cultured in 75 cm 2 Lipid extracts were desiccated for 2 h and total lipid contents determined gravimetrically.…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Transcriptional control mechanisms of genes of lipid and fatty acid metabolism in the Atlantic salmon (Salmo salar L.) established cell line, SHK-1

Minghetti

Leaver

Tocher

2011

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

137

114

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“…It was developed initially for the culture of a salmon viral pathogen, infectious salmon anemia virus (ISAV), and characterized as being adherent with typical macrophage-like morphology. The cells are known to phagocytose the fish bacterial pathogen Aeromonas salmonicida but show minimal antibacterial activity (11). Further characterization of the cell line in relation to major histocompatibility complex (MHC) I and II expression has shown that both MHC I heavy chain and MHC II ␤-chain mRNAs are induced after infection by two different viruses (ISAV and infectious pancreatic necrosis virus), indicating that the cells respond to viral challenge possibly through an IFN signaling mechanism (24).…”

Section: Discussionmentioning

confidence: 99%

Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

Martín

Taggart

Seear

et al. 2007

Physiological Genomics

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Secombes CJ. Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray. Physiol Genomics 32: [33][34][35][36][37][38][39][40][41][42][43][44] 2007. First published September 5, 2007; doi:10.1152/physiolgenomics.00064.2007 RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures. transcriptome; immune; fish INTERFERONS (IFNs) are cytokines that have key roles in the regulation of both innate and adaptive immune responses. They exhibit a diverse range of activities including antiviral, antitumor, and immunomodulatory roles (4). There are three types of IFN known in mammals (I-III); however, to date only types I and II have been identified in fish. Type I consists of a large number of related proteins including the ␣-and ␤-IFNs, which function principally in the antiviral response (55). Type II IFN is a single protein (IFN-␥) that originally was described for its macrophage-activating activity (59). The two families of IFN molecules are not structurally related but share similarities in their receptors and mechanisms of gene activation (41, 55). Type I and II IFNs produce their effect via different heterodimer cell surface receptors. Once IFN is bound to the receptor, signal transduction leads to activation of IFN-responsive genes via conserved signal transduction pathways leading to change of function of the cell (53). It is estimated in mammals that up to 200 or more genes may be affected by IFN stimulation (13). IFN-␥ signal transduction is primarily via the Janus-activated kinase (JAK)-signal transducer and activator of transcription (STAT) pathway. Here JAK1 associates with JAK2 on stimulation and causes the phosphorylation of STAT1. Two STAT1 molecules dimerize to form a homodimer, which then binds to specific consensus sequences in the promoter of IFN-␥-responsive genes at specific ␥-IFN activation site (GAS) elements, resulting in initiation of transcription. Type I IFN molecules follow a similar pathway, but there are two different kinases that are associated with the receptor. In this case JAK1 and tyrosine kinase 2 (TYK2) are activated and phosphorylate both STAT1 and STAT2, which form a heterodimer that in combination with interferon regulatory factor 9 forms a trimer that binds to interferon-stimulated response elements (IRSE) and subsequently induces transcription (reviewed in Refs. 41, 55). In mammals IFN type I can stimulate genes via both IRSE and GAS elements in the promoter, whereas IFN-␥ does not usually act via IRSE (41, 55), although many IFN-respon...

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Characterisation of a long-term cell line (SHK-1) developed from the head kidney of Atlantic salmon (Salmo salarL.)

Cited by 81 publications

References 26 publications

Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

Transcriptional control mechanisms of genes of lipid and fatty acid metabolism in the Atlantic salmon (Salmo salar L.) established cell line, SHK-1

Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

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