Establishment in continuous culture of a T‐lymphoid cell line (HD‐Mar) from a patient with Hodgkin's lymphoma

Ben‐Bassat, Hannah; Mitrani-Rosenbaum, Stella; Gamliel, Haim; Naparstek, Elizabeth; Leizerowitz, Rachel; Korkesh, Aviva; Sagi, Michal; Voss, R.; Kohn, Gertrude; Polliack, Aaron

doi:10.1002/ijc.2910250506

Cited by 35 publications

(11 citation statements)

References 30 publications

(13 reference statements)

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“…Fluorescence polarization studies done in our laboratories have led to the notion that malignancy of lymphocytes, both in experimental animals and humans, is accompanied by a marked decrease in the degree of microviscosity of membrane lipids in leukemic cells as compared to normal lymphocytes (12)(13)(14)(15)(16). In accord with results obtained in model systems (9),…”

supporting

confidence: 77%

Decreased microviscosity of membrane lipids in leukemic cells: Two possible mechanisms

Petitou

Tuy

Rosenfeld

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Steady-state fluorescence polarization studies with the fluorescent lipid probe 1,6-diphenyl 1,3,5-hexatriene were done to determine the degree of microviscosity of cellular membrane lipids and serum lipoproteins in human normal donors and leukemic patients. The results show a marked decrease in microviscosity of cellular membrane lipids in both intact lymphocytes and isolated cellular plasma membranes obtained from leukemic patients in clinical relapse as compared to intact lymphocytes and isolated cellular plasma membranes obtained from normal donors and leukemic patients in complete clinical remission. Concomitant to these dynamic changes in cellular membrane lipids, the degree of microviscosity of lipids in the blood serum of leukemic patients in clinical relapse is markedly reduced as compared to serum obtained from normal donors and leukemic patients in complete clinical remission. Moreover, an in vitro incubation of leukemic lymphocytes with normal low density lipoproteins results in an increased microviscosity of cellular membrane lipids. In addition to the interrelation between cellular membrane lipids and serum lipoproteins, plasma membrane vesicles with a high degree of lipid microviscosity were isolated from the blood serum and pleural effusion of leukemic patients in clinical relapse. Such membrane vesicles could not be detected in normal serum. Therefore, we suggest that the two major mechanisms associated with the decreased microviscosity of membrane lipids in human leukemic cells are an abnormal exchange in lipids between the leukemic cell surface membrane and leukemic serum lipoproteins and an exfoliation of plasma membrane vesicles with a high degree of microviscosity from the cell surface of leukemic cells.The dynamic nature of a biological lipid complex can be quantitatively monitored by fluorescence polarization analysis with the aid of the fluorescent hydrocarbon probe 1,6-diphenyl 1,3,5-hexatriene (DPH) (1-7). From the recorded degree of fluorescence polarization, the degree of microviscosity of the analyzed sample can be estimated (8,9). Results obtained with this method have shown that the major parameter that determined the degree of microviscosity of lymphocyte membranes is the molar ratio of cholesterol to phospholipids, the two main lipid components of membranes in mammalian cells (10,11 the main characteristic that determines this dynamic difference originates from a significant decrease in the molar ratio of cholesterol to phospholipids in the plasma membrane lipid core of leukemic lymphocytes (10). Recently, two hypotheses have been advanced to explain these differences between normal and leukemic lymphocytes: exchange of lipids between cellular membranes and serum lipoproteins (1, 17), and exfoliation of plasma membrane vesicles from the leukemic cells with a high ratio of cholesterol to phospholipids (10, 18). Our main interest in the present study was to determine the degree of microviscosity of lymphocyte membrane lipids and of serum lipids in normal donors and leukem...

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supporting

confidence: 77%

Decreased microviscosity of membrane lipids in leukemic cells: Two possible mechanisms

Petitou

Tuy

Rosenfeld

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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“…All cell lines were grown in RPMI 1640 supplemented with 10% fetal calf serum, 100 U/mI of pencillin and 50 lAg/ml of streptomycin. HDMAR is a T leukaemia cell line (Ben Bassat et al, 1980) and Daudi is a Burkitt lymphoma cell line (Klein et al, 1968).…”

Section: Cellsmentioning

confidence: 99%

The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product.

et al. 1989

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“…The human T cell line HD-Mar, lkndly provided by Dr H. Ben-Bassat (Ben-Bassat et al, 1980), the human B cell lines 209 (a gift of Dr G.Lenoir, Lyon, France) and GM 4672 (Satoh et al, 1983) were grown in RPMI 1640 supplemented with 15% fetal calf serum (FCS). HeLa cells were grown in Dulbecco's modified Eagle's medium containing 10% FCS.…”

Section: Cells and Reagentsmentioning

confidence: 99%

Promoter and enhancer elements in the rearranged alpha chain gene of the human T cell receptor.

et al. 1987

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Establishment in continuous culture of a T‐lymphoid cell line (HD‐Mar) from a patient with Hodgkin's lymphoma

Cited by 35 publications

References 30 publications

Decreased microviscosity of membrane lipids in leukemic cells: Two possible mechanisms

Decreased microviscosity of membrane lipids in leukemic cells: Two possible mechanisms

The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product.

Promoter and enhancer elements in the rearranged alpha chain gene of the human T cell receptor.

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