Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea)

Chen, Kun; Li, Qingfei; Xu, Dan; Zhang, Junzhi; Gao, Shengnan; Xu, Wei; Mai, Kangsen; Ai, Qinghui

doi:10.1016/j.dci.2019.103477

Cited by 37 publications

(21 citation statements)

References 69 publications

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“…A compatible cell culture medium is important to establish a cell line (Ma et al, 2015). Previous studies showed that fish kidney cells could be cultured well in different cell culture media (Cui et al, 2020;Liu et al, 2018;Xue et al, 2017). Both DMEM and DMEM-F12 have been used to culture kidney cells from black carp and large yellow croaker (Cui et al, 2020;Xue et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that fish kidney cells could be cultured well in different cell culture media (Cui et al, 2020;Liu et al, 2018;Xue et al, 2017). Both DMEM and DMEM-F12 have been used to culture kidney cells from black carp and large yellow croaker (Cui et al, 2020;Xue et al, 2017). L-15 has been used for kelp grouper kidney cells culture in vitro (Liu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, we confirmed that FBS was critical to stimulate CrEK proliferation. However, for culture at different stages, varying concentrations of FBS should be used, for example 20% FBS for primary culture and 10% FBS for maintenance, as is the case for other fish cell lines (Cui et al, 2020;Ma et al, 2015). In addition, we determined the chromosome number of CrEK cells in this study.…”

Section: Discussionmentioning

confidence: 99%

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Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

Liu

Fan

Zhou

et al. 2021

Journal of Fish Diseases

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Chinese rice-field eel (Monopterus albus), the family of Synbranchidae, is an economically important species in freshwater fisheries in China.The aquaculture of Chinese rice-field eel has been warmly welcome by farmers and consumers because of its nutritional value and delicious taste (Wu et al., 2016). However, alongside the rapid development of the aquaculture of this specific fish, disease problem has become a great concern during the past years, which resulted in considerable economic losses and posed a significant threat to the sustainable development of the industry (Liu et al., 2019;Ou et al., 2013;Shao et al., 2016).Chinese rice-field eels rhabdovirus (CrERV), belonging to the genus Perhabdovirus in the family Rhabdoviridae, is the causative agent of the haemorrhagic disease of Chinese rice-field eels, Monopterus albus (Liu et al., 2019). The genome size of CrERV is 11,545 bp in length which could encode five major functional proteins, namely (from 3′-5′) a nucleoprotein (N), phosphoprotein (P),

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

Liu

Fan

Zhou

et al. 2021

Journal of Fish Diseases

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“…Macrophage cell lines of the large yellow croaker (LCM) cells ( 13 ) were cultured in DMEM/F12 supplemented with 15% fetal bovine serum (FBS) (Biological Industries, Israel), 100 U/mL penicillin and 100 μg/mL streptomycin at 27°C and 5% CO 2 . HEK 293T cells were cultured in high-glucose DMEM supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37°C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…The large yellow croaker (Larimichthys crocea) is one of the most economically important marine fish and is widely cultured in China. Based on the established method of cell culture and a large number of immunity studies (13)(14)(15), the large yellow croaker can be used as a model for studying the molecular mechanisms of inflammation in teleosts. This study explored the role of SHP in response to inflammation in the large yellow croaker.…”

Section: Introductionmentioning

confidence: 99%

LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)

Xiang

et al. 2021

Front. Immunol.

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The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The shp gene from large yellow croakers, was cloned, and this gene is mainly expressed in the liver and intestine. Lipopolysaccharide (LPS) stimulation induced the mRNA expression and protein level of SHP in macrophages of large yellow croakers. Overexpression of SHP significantly decreased mRNA expression of tnfα, il-1β, il-6 and cox2 induced by LPS treatment in macrophages. LPS stimulation increased the phosphorylation level of Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) in macrophages. AMPK inhibitor treatment significantly decreased the expression of SHP induced by LPS while AMPK activator significantly increased the expression of SHP. The nuclear factor-erythroid 2-related factor 2 (NRF2) increased the promoter activity of SHP in large yellow croakers and the level of nuclear NRF2 was increased by LPS stimulation and AMPK activation. NRF2 inhibitor treatment significantly decreased mRNA expression of shp induced by LPS and AMPK activator. In conclusion, LPS can induce SHP expression by activating the AMPK-NRF2 pathway while SHP could negatively regulate LPS-induced inflammation in large yellow croakers. This study may be benefit to the development of immunology of marine fish and provide new ideas for inflammation-related diseases.

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Fish Macrophages

Wiegertjes

Elks

2022

Principles of Fish Immunology

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Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea)

Cited by 37 publications

References 69 publications

Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)

Fish Macrophages

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