Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, <i>Monopterus albus</i> to the infection of rhabdovirus, CrERV

Liu, Wenzhi; Fan, Yuding; Zhou, Yong; Jiang, Nan; Li, Yiqun; Meng, Yan; Xue, Mingyang; Zhong, Li; Zeng, Lingbing

doi:10.1111/jfd.13563

Cited by 6 publications

(3 citation statements)

References 26 publications

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“… 2019 ), kidney (Liu et al . 2022 ), and gill (Sathiyanarayanan et al . 2022 ) tissue in other teleosts.…”

Section: Discussionmentioning

confidence: 99%

Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus)

Liu

et al. 2023

In Vitro Cell.Dev.Biol.-Animal

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A continuous intestine cell line from turbot (Scophthalmus maximus) designated as SMI was established utilizing the tissue explant technique. Primary SMI cell was cultured at 24 °C in a medium with 20% fetal bovine serum (FBS), then subcultured in 10% FBS after 10 passages. Impacts of medium or temperature on the growth of SMI were examined and the results indicated it grew well in DMEM supplemented with 10% FBS at 24 °C. The SMI cell line was subcultured more than 60 times. Karyotyping, chromosome number, and ribosomal RNA genotyping analysis revealed that SMI had a modal diploid chromosome number of 44 and originated from turbot. After being transfected with pEGFP-N1 and FAM-siRNA, a large number of green fluorescence signals were observed in SMI, indicating that SMI could be used as an ideal platform to explore gene function in vitro. In addition, the expression of epithelium-associated genes such as itga6, itgb4, gja1, claudin1, zo-1, and E-cadherin in SMI suggested the SMI had some characteristics of epidermal cells. The upregulation of immune-associated genes such as TNF-β, NF-κB, and IL-1β in SMI after stimulation with pathogen-associated molecular patterns suggested the SMI might exhibit immune functions similar to the intestinal epithelium in vivo.

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“… 2019 ), kidney (Liu et al . 2022 ), and gill (Sathiyanarayanan et al . 2022 ) tissue in other teleosts.…”

Section: Discussionmentioning

confidence: 99%

Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus)

Liu

et al. 2023

In Vitro Cell.Dev.Biol.-Animal

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“…Chromosome analysis was performed on the 30th passage of the SCC cells as described previously (Liu et al, 2022; Zeng et al, 2022) . Briefly, when the cells were grown in the log‐phase and reached >80% confluence, the cells were treated with 0.4 μg/ml colchicine (Solarbio, China) for 3–4 h. Then the cells were harvested by trypsin digestion and pelleted by centrifugation at 4°C and 1200 g/min for 8 min.…”

Section: Methodsmentioning

confidence: 99%

“…Chromosome analysis was performed on the 30th passage of the SCC cells as described previously (Liu et al, 2022;Zeng et al, 2022) .…”

Section: Chromosomal Analysismentioning

confidence: 99%

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Gong

Lin

et al. 2022

Journal of Fish Diseases

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Siniperca chuatsi, a mandarin fish, is a unique species of freshwater fish in China. It is one of the most economically important freshwater fish due to its delicious taste and high nutritional value. In 2019, the total farmed production of S. chuatsi in China was nearly 320,000 tons, with an output value exceeding RMB 20 billion (Li et al., 2021). Mandarin fish are mainly farmed in Guangdong, Hubei, Jiangxi, Anhui and Jiangsu Provinces of China, with the highest production in Guangdong. The expansion of the scale and density of mandarin fish farming has led to a variety of pathogenic microorganisms causing disease outbreaks in mandarin fish (Sun et al., 2017). A number of bacterial, parasitic and viral diseases have been reported from the mandarin fish. Among the viruses causing infectious diseases in fish, the highly infectious and lethal viruses Infectious Spleen and Kidney Necrosis Virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV), have been reported in the mandarin fish and cause considerable losses to mandarin fish aquaculture. For example, SCRV outbreaks in hundreds of fish farms in Guangdong were reported, which caused death of more than 10 million juvenile mandarin fish (Zhang et al., 2020). Therefore, the prevention and treatment of these viral diseases has become an urgent problem to be solved in the mandarin fish aquaculture.

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Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus

Liu,

Xue,

et al. 2025

Aquaculture

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Susceptibility of a cell line derived from the kidney of Chinese rice‐field eel, Monopterus albus to the infection of rhabdovirus, CrERV

Cited by 6 publications

References 26 publications

Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus)

Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus)

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus

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