Establishing micropropagation conditions for five Cotoneaster genotypes

Monier, Carine; Ochatt, Sergio

doi:10.1007/bf00029999

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…It has been reported that IAA (@ 1.0 mg L -1 ) with MS medium is required for the induction of roots in C. forskohlii . However, the success of an auxin-free basal medium in the present study for efficient root induction (Monier and Ochatt, 1995;Mao et al, 1995) validated our own observations. This discrepancy can be attributed in part to the availability of higher quantity of endogenous auxins present in in vitro regenerated shoot lets (Minocha, 1987).…”

Section: Resultssupporting

confidence: 88%

In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

Krishna

Reddy

Nair³

et al. 2010

Physiol Mol Biol Plants

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Section: Resultssupporting

confidence: 88%

In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

Krishna

Reddy

Nair³

et al. 2010

Physiol Mol Biol Plants

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“…Of the three auxins tried, IBA was found to be most promising growth regulator followed by NAA and IAA. Monier and Ochatt (1995) reported that the highest concentration of auxins resulted maximal rooting in Cotoneaster genotypes. In the present study, the highest number of roots per shoot (4.2 cm) and root length (5.6 cm) were obtained when the half-strength MS medium was supplemented with 0.5 mg L -1 IBA.…”

Section: Discussionmentioning

confidence: 99%

“…Plant tissue culture techniques are considered as easy and reliable methods for rapid propagation of plants, especially rare and endangered plant species (Francis et al 2007;Joshi and Dhawan 2007;Sivanesan and Jeong 2007;Offord and Tyler 2009;Goncalves et al 2010). So far, only two reports are available on the micropropagation of Cotoneaster species (Norton and Boe 1982;Monier and Ochatt 1995). They tested the effect of 6-benzyladenine (BA) alone or in combination with other plant growth regulators.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant

Sivanesan

Song

Hwang

et al. 2010

Plant Cell Tiss Organ Cult

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A simple and efficient micropropagation system was developed for Cotoneaster wilsonii through node and shoot tip explants obtained from mature field-grown plants. Of the two explants, node explants were found to be the most effective for axillary shoot proliferation. The highest frequency of shoot induction was achieved when nodal explants were incubated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L -1 thidiazuron (TDZ) and 0.1 mg L -1 a-naphthaleneacetic acid (NAA) with an average of 34 shoots per explant. The microshoots were separated from the multiple shoots and subcultured on MS medium supplemented with 3% (w/v) sucrose and 0.8% (w/v) agar for further shoot growth. Maximum rooting was obtained on half-strength MS medium supplemented with 0.5 mg L -1 indole-3-butyric acid (IBA). The in vitro-grown plantlets were successfully acclimatized in a glasshouse with 98% of survival. High concentrations of TDZ (1.5-2.0 mg L -1 ) and repeated subcultures resulted hyperhydric shoots. Supplementation of the culture medium with silicon significantly reduced the induction of hyperhydric shoots. Increasing silicon concentration significantly decreased malondialdehyde content of the regenerated shoots. Data indicate that addition of silicon to the culture medium can effectively control hyperhydricity.

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“…Similar observations were reported in Indian spinach (Mitra and Mukherjee 2001). In S. acmela, for root initiation, mineral nutrients and half-strength MS medium was used, as supported by the report of Monier and Ochatt (1995) and Saritha et al (2002). Root induction was best in half strength MS basal medium with 1.0 mg dm -3 of IBA.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 93%

Direct shoot regeneration from leaf explants of Spilanthes acmella

Saritha

Naidu

2008

Biol. plant.

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Multiple shoots of Spilanthes acmella Murr. were induced from nodal buds of in vivo and in vitro seedlings on Murashige and Skoog (MS) medium containing 1.0 mg dm -3 6-benzyladenine (BA) and 0.1 mg dm -3 α-naphthaleneacetic acid (NAA). Adventitious shoots were successfully regenerated from the leaf explants derived from the above mentioned multiple shoots. The efficiency of shoot regeneration was tested in the MS medium containing BA, kinetin, or 2-isopentenyl adenine in combination with NAA, indole-3-acetic acid (IAA), or indole-3-butyric acid (IBA) and gibberellic acid. Maximum number of shoots per explant (20 ± 0.47) was recorded with 3.0 mg dm -3 BA and 1.0 mg dm -3 IAA. An anatomical study confirmed shoot regeneration via direct organogenesis. About 95 % of the in vitro shoots developed roots after transfer to half strength MS medium containing 1.0 mg dm -3 IBA. 95 % of the plantlets were successfully acclimatized and established in soil. The transplanted plantlets showed normal flowering without any morphological variation.Additional key words: growth regulators, medicinal plant, tissue culture. ⎯⎯⎯⎯Spilanthes acmella is an acutely threatened plant species (Narayana Rao and Raja Reddy 1983). S. acmella (marati mogga) is one of the important medicinal plants belonging to the family Asteraceae. The active compound was found to be spilanthol (Ramsewak et al. 1999). It is a perennial herb grown in the tropics and subtropics. Direct regeneration from leaf, as another alternative step for clonal propagation and germplasm conservation, is a well established factor. Successful plant regeneration has been reported from leaf explants, e.g., in Indian spinach (Mitra and Mukherjee 2001), Plumbago species (Das and Rout 2002), safflower (Radhika et al. 2006) and Carthamus species (Sujatha and Dinesh Kumar 2007). In view of the medicinal properties of S. acmella, its threatened nature and the increased demand for it in the pharmaceutical industry, there is a need for a large scale multiplication. In the present study, the direct shoot regeneration and histological studies from leaf explants of S. acmella have been studied. Direct regeneration of this plant species has not been reported so far.Spilanthes acmella Murr. shoots 22 cm long with 5 nodes were collected from 15-week-old field grown plants raised from seeds. They were divided into shoot tips and nodes, each measuring about 1 -4 cm in length.These served as explants for multiple shoot production. Explants were initially washed in running tap water for 15 min, and then rinsed in 10 % Teepol for 5 min followed by 0.1 % Bavistin for 5 min. The explants were thoroughly washed with sterile distilled water four times. Surface sterilization was carried out in 70 % ethanol for 45 s. Then the explants were treated with 0.05 % HgCl 2 for 4 min and 5 -6 times rinses in sterile distilled water. The nodal and shoot tip explants were inoculated in the Murashige and Skoog (1962; MS) medium fortified with 1.0 mg dm -3 6-benzyladenine (BA) and 0.1 mg dm -3 α-naphthalene acetic acid...

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Establishing micropropagation conditions for five Cotoneaster genotypes

Abstract: Micropropagation from shoot tips of five Cotoneaster genotypes C. franchetii, C dammeri var. radicans, C.

Cited by 9 publications

References 10 publications

In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant

Direct shoot regeneration from leaf explants of Spilanthes acmella

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