In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

Comprehensive review on phytochemistry and in vitro biotechnology of Coleus forskohlii

“…The regeneration of this medicinally important species can be achieved employing the selection of any one of the explants namely, leaf (proximal, middle, distal) [2,& 8] , nodal segment [19] shoot tip [20] . A high frequency shoot organogenesis and plant establishment protocol has been developed for Coleus forskohlii from leaf derived callus [2] . Nodal segments of Coleus species were observed to be the most appropriate explant source for initiation of cultures, since they withstood sterilization process better than apical shoots, exhibiting maximum survival frequency [21] .…”

“…High multiplication efficiency, phenotypic stability and yield were ensured [22] . Leaf explants that were excised into distal segments i.e., proximal (P), middle (M) and distal (D) when transferred to the nutrient medium, showed regeneration by elongated shoots and profuse rooting [2] . Comparison of shoot regeneration response from different leaf segments at 5.0 mg/l BAP showed that the distal end was comparatively higher with 45 shoots than other leaf segments.…”

“…Optimal callus was developed from mature leaves on MS medium supplemented with 2.4μM kinetin alone. Shoots were regenerated from the callus on MS medium supplemented with 4.6 μM kinetin and 0.54 μM 1naphthalene acetic acid [2] . Within 35-40 days by culturing stem tip explants in MS medium containing through direct multiplication at the rate of 12.5 shoots per explant.…”

“…is an essential subtropical, warm temperate medicinal plant. The species is known for its highly valued labdane diterpenoid alkaloid 'forskolin', which is mainly present in the brownish-red tuberous roots of this plant that is the only source of forskolin [1] The root extracts of the plant are rich in forskolin (0.07 to 0.59%) and have many pharmaceutical properties such as anti-inflammatory, antiglaucoma, antithrombotic, antiplatelet aggregation, antidepressant and antidiuretic activities [2][3][4][5] . The root of this plant is medicinally useful for high blood pressure, spasmolysis, obesity and constipation [6] .…”

Adventitious shoot regeneration from leaf and stem explants of Salix matsudana Koidz

“…The cytokinin-auxin combination has been used widely for shoot regeneration in varieties of protocols performed for other species, such as Trifolium alexandrinum L. (Abogadallah and Quick 2010), Populus euphratica (Zhou et al 2012), Malus zumi (Xu et al 2008), Paulownia tomentosa (Corredoira et al 2008) and so on. In endangered multipurpose medicinal plant Coleus forskohlii, the optimal direct shoots regeneration (76.4%) was obtained in MS medium with 5.0 mg/l BAP after 28 days incubation (Krishna et al 2010). The present results showed that 1.0 mg/l of BAP was optimal for the calli and shoots induction.…”