Direct shoot regeneration from leaf explants of Spilanthes acmella

Saritha, K.; Naidu, C. V.

doi:10.1007/s10535-008-0068-6

Cited by 33 publications

(18 citation statements)

References 15 publications

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“…The abaxial epidermal cells increased in size and simultaneously divided into several planes which formed meristemoids on the surface of the midrib. These meristemoids, by further cell divisions gave rise to small protrusions of tissue, which gradually organized into shoot buds similarly as described by Saritha and Naidu (2008) in Spilanthes acmella.…”

supporting

confidence: 53%

Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Khurana-Kaul¹,

Kachhwaha²,

Kothari³

2010

Biol. plant.

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Leaf explants of Jatropha curcas cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ; 0.90 µM) in combination with indole-3-butyric acid (IBA; 0.98µM) produced adventitious shoot buds directly on the surface of the explants without formation of intervening callus while shoot bud formation was accompanied with callus formation on medium supplemented with 6-benzylaminopurine (BAP; 13.3 µM) and IBA (2.46 µM). TDZ treatment resulted in more than twice higher rate of shoot bud induction than BAP. Shoot buds were multiplied and elongated following repeated transfers to medium containing BAP (2.22 µM) and gibberellic acid (GA 3 ; 1.44 µM). The effect of copper sulphate on differentiation of shoot buds from leaf segments was also investigated. Both shoot induction and multiplication media were supplemented with different levels of CuSO 4 (0 -5 µM). Significant improvement in shoot bud induction was observed when the concentration of CuSO 4 was increased to 10 times the normal MS level. Healthy elongated shoots were rooted on half strength MS medium supplemented with IBA (2.46 µM). Rooted plantlets were transferred to field and survived. Histological analysis revealed direct formation of shoot buds from leaf explants.Additional key words: adventitious bud regeneration, benzylaminopurine, gibberellic acid, indole-3-butyric acid, mineral nutrition. ⎯⎯⎯⎯The use of vegetable oil as biofuel is being considered as a suitable alternative to limited fossil fuel reserves. In this context, non-edible vegetable oil of Jatropha curcas has the potential of providing a promising and commercially viable alternative to petro-diesel (Gubitz et al. 1999). Jatropha curcas (family: Euphorbiaceae), is a multipurpose, drought resistant, perennial plant of Latin American origin, but it is now widespread throughout the tropical regions of the world (Openshaw 2000). Seeds are genetically heterozygous as Jatropha is cross pollinated, which results in a high degree of variation (Ginwal et al. 2005). To meet the large scale demand and ensure easy supply of the elite plant material, there is need to establish mass multiplication techniques. Though there are reports regarding the effect of different concentrations and combinations of plant growth regulators on regeneration from various explants in J. curcas Mukta 1996, Datta et al. 2007, Deore and Johnson 2008), attempts have not been made to standardize the nutrient requirement to improve regeneration. Primarily, the composition and ratios of plant growth regulators are manipulated to optimize the quality and number of organs initiated (Ramage and Williams 2002). However, a tissue growth rate and the extent and quality of morphogenic response are also influenced by the type and concentration of nutrients supplied in the culture medium (Niedz and Evens 2007). In the present investigation, we have developed an efficient regeneration protocol by standardizing the copper requirements for improved shoot bud induction.Young and fully-expanded leaves from apex were collected fr...

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supporting

confidence: 53%

Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Khurana-Kaul¹,

Kachhwaha²,

Kothari³

2010

Biol. plant.

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“…On the contrary positive synergistic effect of cytokinins like BAP or Kn or TDZ or 2iP or zeatin in combination with an auxin like IAA, or NAA on in vitro shoot regeneration through organogenic differentiation has been reported by several workers (Shen et al 2007, Unda et al 2007, Erisen et al 2010, Ghimire et al 2010, Lin et al 2011, Wadl et al 2011, Mandal and Laxminarayana 2012, Perez-Jimenez et al 2012and Bhagya et al 2013. Superior effect of BAP for shoot multiplication has been reported among various medicinal plants of Asteraceae, like Tanacetum cinerariifolium (Hedayat et al 2009), Spilanthes acmella (Saritha and Naidu 2008), Launaea sarmentosa (Mahesh et al 2012). As in the present study, the reduction of shoot regeneration potential by the combined effect of BAP and GA 3 compared to a medium with cytokinin alone was also demonstrated in Guizotia abyssinica (Baghel and Bansal 2014).…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA 3 . The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA 3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.

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“…In this work, the leaf explants produced buds mainly from the petiole base. Saritha and Naidu (2008) cultured Spilanthes acmella on MS medium supplemented with different plant growth regulators for direct regeneration from mature leaves or leaves of in vitro grown plantlets. The formation of shoots was observed after 45 d either in the midrib region or basal petiole region or apical region of the leaf.…”

Section: Indirect Organogenesis From Cotyledonary Leaves and Bud Multmentioning

confidence: 99%

Organogenesis and Agrobacterium tumefaciens-mediated transformation of Eucalyptus saligna with P5CS gene

Dibax¹,

Deschamps²,

Filho³

et al. 2010

Biol. plant.

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The purpose of this research was Eucalyptus saligna in vitro regeneration and transformation with P5CSF129A gene, which encodes Δ 1 -pyrroline-5-carboxylate synthetase (P5CS), the key enzyme in proline biosynthesis. After selection of the most responsive genotype, shoot organogenesis was induced on leaf explants cultured on a callus induction medium (CI) followed by subculture on a shoot induction medium (SI). Shoots were subsequently cultured on an elongation medium (BE), then transferred to a rooting medium and finally transplanted to pots and acclimatized in a greenhouse. For genetic transformation, a binary vector carrying P5CSF129A and uidA genes, both under control of the 35SCaMV promoter, was used. Leaves were co-cultured with Agrobacterium tumefaciens in the dark on CI medium for 5 d. The explants were transferred to the selective callogenesis inducing medium (SCI) containing kanamycin and cefotaxime. Calli developed shoots that were cultured on an elongation medium for 14 d and finally multiplied. The presence of the transgene in the plant genome was demonstrated by PCR and confirmed by Southern blot analysis. Proline content in the leaves was four times higher in transformed than in untransformed plants while the proline content in the roots was similar in both types of plants.Additional key words: abiotic stress, in vitro culture, PCR, proline, Southern blot.

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Direct shoot regeneration from leaf explants of Spilanthes acmella

Cited by 33 publications

References 15 publications

Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Organogenesis and Agrobacterium tumefaciens-mediated transformation of Eucalyptus saligna with P5CS gene

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