Essential oils from Vitex trifolia as an effective repellent for Aedes aegypti

Abstract

Abstract. Arpiwi NL, Muksin IK, Kriswiyanti E. 2020. Essential oils from Vitex trifolia as an effective repellent for Aedes aegypti. Biodiversitas 21: 4536-4544. Vitex trifolia is a shrub or small tree grown wild from family Verbenaceae commonly found in the sandy shore habitat of the tropicals and sub-tropical countries. This research aims to measure the essential oils yield of V. trifolia leaves, to investigate the secretory cells of the leaf, and to measure repellent activity of the essential oils against Aedes aegypti. Leaves and soil samples were taken from Panjer, Sanur, and Sidakarya Villages of Denpasar, Bali Indonesia. Oil was extracted from fresh leaf samples using steam distillation. The essential oils were analyzed for its constituents using Gas Chromatography-Mass Spectrometry. The leaf cross-sections were prepared using paraffin embedding method and stained with safranin. The essential oils of V. trifolia at different concentrations (0, 2, 3, 4, 5, 6% w/w) were formulated into lotions. The yield of essential oils was significantly different from the three locations. The main constituent of the oil was cis-ocimene. The essential oil yield was positively correlated with the sand content. The secretory structures of the leaf were idioblast and glandular trichome. Lotions formulated with the essential oils were soft, not greasy, and not sticky upon application to the skin and non-irritant. Lotions with 5 and 6% essential oils gave 100% protection for 3 hours from Ae. aegypti.

Phytochemical Profile, In Vitro Bioactivity Evaluation, In Silico Molecular Docking and ADMET Study of Essential Oils of Three Vitex Species Grown in Tarai Region of Uttarakhand

“…β-caryophyllene is also identified as the major constituent of V. trifolia oil by several other reports [ 56 , 57 , 58 ], which is in agreement with the present study. Arpiwi et al [ 59 ] detected five components in V. trifolia essential oil in which cis -ocimene (44.57%), α-thujene (25.63%) and cyclopentene,3-isopropenyl-5,5-dimethyl (18.19%) were identified as the major constituents. However, in the present study such compounds were not detected, and the amount detected for α-thujene was also negligible (0.2%).…”

Profil Senyawa Metabolit Sekunder dalam Minyak Atsiri Tumbuhan Legundi (Vitex trifolia Linn) yang Tumbuh di Pulau Timor

“…Tabel 4 menunjukkan data senyawa-senyawa minyak atsiri hasil analisis dari sampel daun legundi yang digunakan dalam keadaan kering oleh peneliti-peneliti sebelumnya. Pada penelitian yang dilakukan oleh Arpiwi et al [23] dari Bali, cis-Ocimene merupakan senyawa dominan dengan persentase area sebesar 44,57%. Pada penelitian yang dilakukan oleh Thomas [24] dari Kottayam/Kerala diperoleh Kariofilen sebagai senyawa dominan dengan persentase area sebesar 38,38%.…”

Komunikasi Singkat: Analisis kualitas minyak atsiri daun Melaleuca trichostachya Lindl. dan daya hambatnya terhadap jamur Candida albicans

“…Minyak atsiri dianalisis senyawa penyusunya menggunakan Gas Chromatography-Mass Spectrometry (GC-MS, Shimadzu-QP2010 Ultra) mengikuti metode Arpiwi et al, (2020b). Sampel minyak (20 µL) diencerkan dengan menggunakan 5 mL etanol absolut kemudian disaring dengan kertas saring Whatman no 1.…”