Essential amino acid index of sow’s colostrum

Abstract

The aim of this study was to determine the protein quality of sow's colostrum. For this purpose, the essential amino acid index (EAAI) of sow's colostrum was calculated. The study was carried out on 12 Large white sows, which were on the 2.9 farrowing on average. Colostrum samples were collected by milking per hand without application of oxytocin. First sample were milked at time of birth of first piglet, and then every hour till 12 th hour. Essential amino acids were determined using Amino Acid Analyser AAA400 (Ingos, Prague). For calculation of EAAI, the egg protein (EAAIegg) and protein of sow's milk (EAAIsow's milk) were used as standard. The highest value of EAAI was calculated for both at 4 th h, EAAIegg 97.1%, EAAIsow's milk 120.2%. The lowest values of EAAI were calculated at 12 th h EAAIegg 78.3% and EAAIsow's milk 96.9%. During the first 12 hours, protein of sow's colostrum had not reached the quality of egg protein. Except sample collected at 12 th hour after birth of first piglet, all colostrum samples had better quality of protein in comparison to sow's milk. No significant (P>0.05) differences of EAAI between colostrum samples collected at different times from the birth of the first piglet till 12 th h were observed.Keywords: amino acids, colostrum, index of essential amino acid, sow Abstrakt Cieľom tejto práce bolo stanoviť kvalitu bielkovín v mledzive prasníc. Pre dosiahnutie vytýčených cieľov bol pre mledzivo prasníc vypočítaný index esenciálnych aminokyselín (EAAI). Štúdia bola realizovaná na 12 prasniciach plemena Biela ušľachtilá, ktoré boli v priemere na 2,9 vrhu. Vzorky mledziva boli odoberané ručným oddojením, bez aplikácie oxytocínu. Prvá vzorka bola odobraná v čase narodenia prvého prasiatka vo vrhu, následné vzorky boli odoberané každú hodinu až do dvanástej hodiny po narodení prvého prasiatka. Obsah esenciálnych aminokyselín bol stanovený na prístroji Amino Acid Analyser AAA400 (Ingos, Praha). Ako štandardná bielkovina pre výpočet EAAI bola použitá bielkovina vajca (EAAIegg) a bielkovina mlieka prasníc (EAAIsow's milk). Najvyššie hodnoty EAAI boli stanovené pri oboch na 4 hodinu od narodenia prvého prasiatka, EAAIegg 97,1%, EAAIsow's milk Original scientific paper DOI: /10.5513/JCEA01/19.1.2028 Journal of Central European Agriculture, 2018, 19(1), p.95-101 95 120,2%. Najnižšie hodnoty EAAI boli stanovené na dvanástu hodinu od narodenia prvého prasiatka, EAAIegg 78,3% and EAAIsow's milk 96,9%. Počas sledovaných prvých dvanástich hodín mledzivo prasníc nedosiahlo kvalitu bielkovín, ktorá je vo vaječnej bielkovine. Okrem vzorky mledziva odobranej na dvanástu hodinu po narodení prvého prasiatka, mali všetky vzorky mledziva lepšiu kvalitu bielkovín ako je kvalita bielkovín v mlieku prasníc. Rozdiely hodnôt EAAIegg a aj EAAIsow's milk zistené medzi jednotlivými časmi odberu vzorky boli štatisticky nepreukazné (P>0.05).

New Challenges and Future Perspectives in Nutrition and Sustainable Diets in Africa

“…These methods included the Chemical Score of the Essential Amino Acids (CSEAA) or CS (Mitchell and Block, 1946;Eq. 1), the Essential Amino Acid Index (IEAA; Oser, 1959;Rolinec et al, 2017;Huang et al, 2018;Abdualrahman et al, 2019;Eq. 2)…”

Protein Quality of South African Potatoes to Inform Dietary Choices

“…The essential amino acid index (EAAI) measures the presence of amino acids that the human body cannot synthesise and gives a stronger indication of potential nutritive value. The essential amino acids index (IEAA) was calculated using the following equation (Eq 2) described by various researchers (Oser 1959;Huang et al 2018;Rolinec et al 2018;Abdualrahman et al 2019).…”

The energetic, immunology and amino acids quality of sow's colostrum – a review

“…Study published by Rolinec et al [2018] aimed to calculation of essential amino acid index (EAAI) of sow's colostrum. EAAI determined the quality of protein in a sample, as a reference protein the egg protein is considered.…”

“…No one of the sow's colostrum samples gained during first 12 hours after birth of first piglet achieve the protein quality of egg. Colostrum sampled at 4th and 7th hour after birth of first piglet have the highest protein quality with EAAI value 97.1 and 96.8, respectively [Rolinec et al 2018].…”