Esculetin antagonizes iron-chelating agents and increases the virulence of Listeria monocytogenes

Coulanges, Valerie; André, Philippe; Vidon, Dominique J.-M.

doi:10.1016/s0923-2508(97)85115-7

Cited by 18 publications

(15 citation statements)

References 21 publications

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“…We consider the following possibilities for this multifunctional recognition of siderophores: (i) there is a single receptor with different recognition sites or a single receptor that recognizes a common structural feature between all of the different siderophores; (ii) there are many receptors but a common periplasmic binding protein; (iii) the ferric-siderophore delivers the iron to a surface-bound iron carrier (likely through a reduction process), with the siderophore itself remaining outside the cell, as is observed in some fungi (35) and Listeria monocytogenes (36); or (iv) there are several transport systems, but the protein TonB is present in limiting amount and is therefore unable to promote siderophore uptake. Our results show that there is a single receptor, as we now describe.…”

Section: Resultsmentioning

confidence: 99%

Microbial iron transport via a siderophore shuttle: A membrane ion transport paradigm

Stintzi

Barnes

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

229

184

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A mechanism of ion transport across membranes is reported. Microbial transport of Fe 3؉ generally delivers iron, a growthlimiting nutrient, to cells via highly specific siderophore-mediated transport systems. In contrast, iron transport in the fresh water bacterium Aeromonas hydrophila is found to occur by means of an indiscriminant siderophore transport system composed of a single multifunctional receptor. It is shown that (i) the siderophore and Fe 3؉ enter the bacterium together, (ii) a ligand exchange step occurs in the course of the transport, and (iii) a redox process is not involved in iron exchange. To the best of our knowledge, there have been no other reports of a ligand exchange mechanism in bacterial iron transport. The ligand exchange step occurs at the cell surface and involves the exchange of iron from a ferric siderophore to an iron-free siderophore already bound to the receptor. This ligand exchange mechanism is also found in Escherichia coli and seems likely to be widely distributed among microorganisms.

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Section: Resultsmentioning

confidence: 99%

Microbial iron transport via a siderophore shuttle: A membrane ion transport paradigm

Stintzi

Barnes

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

229

184

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show abstract

“…Overnight cultures (2%) were also inoculated into BHI with 5% ethanol, and growth was determined manually with a Beckman 640 spectrophotometer. Iron deprivation was achieved through the addition of tropolone to BHI (10). After its addition, the broth was left shaking overnight at 37°C to allow the tropolone to bind the iron and then used the following day for inoculation.…”

Section: Methodsmentioning

confidence: 99%

“…Esculetin has a high iron-binding ability and makes iron available for utilization by L. monocytogenes during infection. This results in a substantial increase in sensitivity to infection and a significant reduction in lethal dose (10). Esculetin can supply iron to L. monocytogenes EGDe to facilitate normal growth in complex media depleted of iron (Fig.…”

Section: Vol 72 2004mentioning

confidence: 99%

Disruption of Putative Regulatory Loci in Listeria monocytogenes Demonstrates a Significant Role for Fur and PerR in Virulence

2004

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The ability to adapt to adverse environmental conditions encountered in food and during host infection is a sine qua non for a successful Listeria monocytogenes infection. This ability is likely to depend on complex regulatory pathways controlled by a number of key regulators. We utilized the pORI19 plasmid integration system to analyze the role of six putative regulatory loci in growth under suboptimal environmental conditions and during murine infection. Disruption of loci encoding a topoisomerase III (lmo2756), a putative methyltransferase (lmo0581), and a regulator of the MarR family (lmo1618) revealed roles for the methyltransferase and the MarR regulator in growth under environmental stress conditions. However, plasmid integration into these loci had no impact on virulence potential in the murine model of infection. Disruption of the alternative sigma factor Sigma-H resulted in a mutant that demonstrated reduced growth potential in minimal medium. Murine studies indicated a minor role for this sigma factor in the infectious process. Strikingly, disruption of both perR and fur loci resulted in mutants that are significantly affected in virulence for mice, with the fur mutant demonstrating the greatest reduction in virulence potential. Both perR and fur mutants demonstrated increased resistance to hydrogen peroxide and the fur mutant was sensitive to low-iron conditions. The virulence defect of both fur and perR mutants could be rescued by iron-overload after esculetin treatment of mice, suggesting that the in vivo role of these gene products is to procure iron for bacterial growth.

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“…This particular structure resembles that of catechol-type siderophores produced and released by bacteria or fungi to mobilize and chelate Fe (Hider and Kong, 2010). Interestingly, some bacteria do not synthesize but utilize esculetin as exogenous siderophore to acquire Fe (Coulanges et al, 1996). In this case, microorganisms produce esculetin mainly from the hydrolysis of plant-derived esculin.…”

Section: Esculetin As a Prototype For Fe(iii)-mobilizing Catechols Sementioning

confidence: 99%

Feruloyl-CoA 6'-Hydroxylase1-Dependent Coumarins Mediate Iron Acquisition from Alkaline Substrates in Arabidopsis

Schmid¹,

Giehl²,

Döll³

et al. 2013

PLANT PHYSIOLOGY

283

383

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Esculetin antagonizes iron-chelating agents and increases the virulence of Listeria monocytogenes

Cited by 18 publications

References 21 publications

Microbial iron transport via a siderophore shuttle: A membrane ion transport paradigm

Microbial iron transport via a siderophore shuttle: A membrane ion transport paradigm

Disruption of Putative Regulatory Loci in Listeria monocytogenes Demonstrates a Significant Role for Fur and PerR in Virulence

Feruloyl-CoA 6'-Hydroxylase1-Dependent Coumarins Mediate Iron Acquisition from Alkaline Substrates in Arabidopsis

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