Disruption of Putative Regulatory Loci in
            <i>Listeria monocytogenes</i>
            Demonstrates a Significant Role for Fur and PerR in Virulence

Rea, Rosemarie B.; Gahan, Cormac G. M.; Hill, Colin

doi:10.1128/iai.72.2.717-727.2004

Cited by 109 publications

(98 citation statements)

References 52 publications

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“…Furthermore, three irondependent regulators belonging to the Fur family (LSA0109, LSA0466, LSA1029) were observed, as is the case for numerous Gram-positive pathogens, but not for other lactobacilli. Although the exact role of these regulators is unclear, they might be involved in resistance to oxidative stress or regulation of iron transport proteins 29,30 .…”

Section: Heme and Iron Requirementmentioning

confidence: 99%

The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

Chaillou¹,

Champomier‐Vergès²,

Cornet³

et al. 2005

Nat Biotechnol

352

307

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Section: Heme and Iron Requirementmentioning

confidence: 99%

The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

Chaillou¹,

Champomier‐Vergès²,

Cornet³

et al. 2005

Nat Biotechnol

352

307

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“…L. monocytogenes EGD was obtained from Werner Goebel (Biozentrum). The sigB mutant derivative was obtained from Lone Brøndsted (Brondsted et al, 2003), and the perR mutant was constructed as described by Rea et al (2004). Strains were grown in rich growth medium (brain heart infusion, BHI; Oxoid) or improved minimal medium (Phan-Thanh & Gormon, 1997), with continuous shaking at 37 uC unless otherwise indicated.…”

Section: Methodsmentioning

confidence: 99%

“…To determine if the regulatory elements identified in the L. monocytogenes fri promoter region are involved in controlling fri expression, we performed a primer extension analysis of wild-type, sigB (Brondsted et al, 2003) and perR (Rea et al, 2004) mutant cells grown to exponential phase in BHI (Fig. 1C).…”

Section: Expression Of L Monocytogenes Fri Ismentioning

confidence: 99%

The Dps-like protein Fri of Listeria monocytogenes promotes stress tolerance and intracellular multiplication in macrophage-like cells

Olsen¹,

Larsen²,

Gahan

et al. 2005

Microbiology

Self Cite

103

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Members of the ferritin-like Dps protein family are found in a number of bacterial species, where they demonstrate the potential to bind iron, and have been implicated in tolerance to oxidative stress. In this study of the food-borne pathogen Listeria monocytogenes, the fri gene encoding a Dps homologue was deleted, and, compared to wild-type cells, it was found that the resulting mutant was less resistant to hydrogen peroxide, and demonstrated reduced survival following long-term (7–11 days) incubation in laboratory media. In view of this, it is shown that fri gene expression is controlled by the hydrogen peroxide regulator PerR, as well as the general stress sigma factor σ B. When fri mutant cells were transferred to iron-limiting conditions, growth was retarded relative to wild-type cells, indicating that Fri may be required for iron storage. This notion is supported by the observation that the L. monocytogenes genome appears not to encode other ferritin-like proteins. Given the role of Fri in resistance to oxidative stress, and growth under iron-limiting conditions, the ability of the fri mutant to infect mice was examined. When injected by the intraperitoneal route, the fri mutant demonstrated a reduced capacity to proliferate in the organs of infected mice relative to the wild-type, whereas when the bacteria were supplied intravenously this effect was mitigated. In addition, the mutant was impaired in its ability to survive and grow in J774.A1 mouse macrophage cells. Thus, the data suggest that Fri contributes to the ability of L. monocytogenes to survive in environments where oxidative stress and low iron availability may impede bacterial proliferation.

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“…PerR homologs have been identified in several pathogenic bacteria and investigated in a few, specifically, S. pyogenes (118), L. monocytogenes (180), and S. aureus (96,97). The inactivation of PerR in all of these bacteria results in an attenuation of virulence and increased resistance to peroxide-induced stress (96,180,185).…”

Section: Perrmentioning

confidence: 99%

At the Crossroads of Bacterial Metabolism and Virulence Factor Synthesis in Staphylococci

Somerville

Proctor

2009

Microbiol Mol Biol Rev

327

348

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SUMMARY Bacteria live in environments that are subject to rapid changes in the availability of the nutrients that are necessary to provide energy and biosynthetic intermediates for the synthesis of macromolecules. Consequently, bacterial survival depends on the ability of bacteria to regulate the expression of genes coding for enzymes required for growth in the altered environment. In pathogenic bacteria, adaptation to an altered environment often includes activating the transcription of virulence genes; hence, many virulence genes are regulated by environmental and nutritional signals. Consistent with this observation, the regulation of most, if not all, virulence determinants in staphylococci is mediated by environmental and nutritional signals. Some of these external signals can be directly transduced into a regulatory response by two-component regulators such as SrrAB; however, other external signals require transduction into intracellular signals. Many of the external environmental and nutritional signals that regulate virulence determinant expression can also alter bacterial metabolic status (e.g., iron limitation). Altering the metabolic status results in the transduction of external signals into intracellular metabolic signals that can be “sensed” by regulatory proteins (e.g., CodY, Rex, and GlnR). This review uses information derived primarily using Bacillus subtilis and Escherichia coli to articulate how gram-positive pathogens, with emphasis on Staphylococcus aureus and Staphylococcus epidermidis, regulate virulence determinant expression in response to a changing environment.

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Disruption of Putative Regulatory Loci in Listeria monocytogenes Demonstrates a Significant Role for Fur and PerR in Virulence

Cited by 109 publications

References 52 publications

The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

The Dps-like protein Fri of Listeria monocytogenes promotes stress tolerance and intracellular multiplication in macrophage-like cells

At the Crossroads of Bacterial Metabolism and Virulence Factor Synthesis in Staphylococci

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