The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

Chaillou, Stéphane; Champomier‐Vergès, Marie‐Christine; Cornet, Monique; Coq, Anne‐Marie Crutz‐Le; Dudez, Anne-Marie; Martin, Véronique; Beaufils, Sophie; Darbon-Rongère, Emmanuelle; Bossy, Robert; Loux, Valentin; Zagorec, Monique

doi:10.1038/nbt1160

Cited by 352 publications

(343 citation statements)

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“…An internal 474-bp fragment of the 16S rRNA gene and an internal 424-bp fragment of the katA gene (encoding the L. sakei heme-dependent catalase) were amplified using primers Y1 (45) and R518 and primers 702-F and 310-R (2), respectively (Table 1). The resulting nucleotide sequences showed to be identical to the corresponding partial sequences in L. sakei 23K (6). L. sakei 23K, a laboratory strain originally isolated from sausage and cured of plasmids (3), was used as the recipient strain for genetic constructions.…”

Section: Methodsmentioning

confidence: 99%

“…It is found in kimchi, silage, cheese, sauerkraut, sourdough, and smoked fish but is mainly found in meat products (4,7,8). Though some L. sakei strains have been identified as responsible for the spoilage of vacuum-packaged meat products, this bacterium is widely used as a starter culture for the production of fermented sausages and has biotechnological potential for biopreservation and food safety (6). Lactobacilli are generally recognized as safe and they are not responsible for human infections in healthy people (46).…”

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confidence: 99%

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Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmidcarried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 g/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 g/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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“…The names are based on two conserved WxL motifs present in both domains (Chaillou et al, 2005;Kleerebezem et al, 2003). The WxL family was divided into two subfamilies, WxL1 and WxL2, on the basis of sequence characteristics of the domains and the proteins containing the domain.…”

Section: Novel Domainsmentioning

confidence: 99%

The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment

et al. 2006

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The predicted extracellular proteins of the bacterium Lactobacillus plantarum were analysed to gain insight into the mechanisms underlying interactions of this bacterium with its environment. Extracellular proteins play important roles in processes ranging from probiotic effects in the gastrointestinal tract to degradation of complex extracellular carbon sources such as those found in plant materials, and they have a primary role in the adaptation of a bacterium to changing environmental conditions. The functional annotation of extracellular proteins was improved using a wide variety of bioinformatics methods, including domain analysis and phylogenetic profiling. At least 12 proteins are predicted to be directly involved in adherence to host components such as collagen and mucin, and about 30 extracellular enzymes, mainly hydrolases and transglycosylases, might play a role in the degradation of substrates by L. plantarum to sustain its growth in different environmental niches. A comprehensive overview of all predicted extracellular proteins, their domains composition and their predicted function is provided through a database at http://www.cmbi.ru.nl/secretome, which could serve as a basis for targeted experimental studies into the function of extracellular proteins.

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“…The Lactobacillus acidophilus NCFM genome sequence of 1?99 Mb revealed the genetic basis for the biochemical complexity and host-interaction capability of this probiotic strain, which has been employed extensively by the dairy industry. The recent genome sequence determination for Lactobacillus sakei strain 23K (Chaillou et al, 2005) provided a contrasting insight into the adaptive mechanisms of this meat-associated organism with extensive auxotrophy, including purine nucleoside scavenging, resilience against changing redox and oxygen levels, and potential mechanisms for biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Diversity of the genus Lactobacillus revealed by comparative genomics of five species

et al. 2006

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The genus Lactobacillus contains over 80 recognized species, and is characterized by a high level of diversity, reflected in its complex phylogeny. The authors' recent determination of the genome sequence of Lactobacillus salivarius means that five complete genomes of Lactobacillus species are available for comparative genomics: L. salivarius, L. plantarum, L. acidophilus, L. johnsonii and L. sakei. This paper now shows that there is no extensive synteny of the genome sequences of these five lactobacilli. Phylogeny based on whole-genome alignments suggested that L. salivarius was closer to L. plantarum than to L. sakei, which was closest to Enterococcus faecalis, in contrast to 16S rRNA gene relatedness. A total of 593 orthologues common to all five species were identified. Species relatedness based on this protein set was largely concordant with genome synteny-based relatedness. A Lactobacillus supertree, combining individual phylogenetic trees from each of 354 core proteins, had four main branches, comprising L. salivarius-L. plantarum; L. sakei; E. faecalis; and L. acidophilus-L. johnsonii. The extreme divergence of the Lactobacillus genomes analysed supports the recognition of new subgeneric divisions.

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The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K

Cited by 352 publications

References 50 publications

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment

Diversity of the genus Lactobacillus revealed by comparative genomics of five species

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