Erythropoiesis with Particular Reference to its Study by Biopsy of Human Bone Marrow: A Review

Culture of Human Bone Marrow in vitro: The Reversibility between Normoblastic and Megaloblastic Series of Cells

“…Megaloblasts.-The cells which are characteristic for pernicious anaemia in relapse and are never found in normal marrows or in marrows other than liver factor or folic acid deficiency (Ehrlich and Lazarus, 1898;Naegeli, 1931;Jones, 1943Jones, , 1947Jones, , 1948Dacie and White, 1949).…”

Addisonian Pernicious Anaemia without Gastric Atrophy in a Young Man

“…It can be seen that he still had a mild macrocytic anaemia and that his serum B,, concentration was subnormal, being similar to that seen in moderately severe B,, deficiency Ross, 1952, 1954a). A sternal bone-marrow aspiration performed 28 days after an injection of liver extract revealed the typical findings of a mild megaloblastic anaemia (Dacie and White, 1949). It was therefore apparent that in spite of treatment with z ml.…”

Sir John Dacie MD, FRS, FRCP, FRCPath

“…In 1947 John White, then an assistant in Pathology at the Hammersmith had been studying cytoplasmic basophilia of bone marrow cells as an indicator of ribonucleic acid, and noted its loss in erythroid cells as they matured. Dacie's interest in morphology resulted in a major review of the morphology of erythropoiesis in bone marrow obtained by needle‐puncture biopsies with the Salah needle (Dacie & White, 1949). They developed a method for concentrating the marrow by placing a drop on a glass slide, sucking off most of the blood with a Pasteur pipette and then spreading the fragments with a smooth edged glass spreader – a technique which is still used successfully in routine marrow aspirations.…”