Culture of Human Bone Marrow in vitro: The Reversibility between Normoblastic and Megaloblastic Series of Cells

Erythroid Cell Proliferation in Human Bone Marrow Suspension Cultures

“…It is now well established that in the absence of erythropoietin no erythroid cell differentiation can be observed in human bone marrow maintained in vitro (Lajtha 1952;Berman & Powsner, 1959;Erslev & Hughes, 1960;Astaldi, 1961;Myhre, 1964a, b, c, d;Golde & Cline, 1973). The results in the experiments described here confirmed these findings, erythroid cells disappearing from culture after 4-5 days.…”

Haemoglobin Synthesis in Human Fetal Liver Maintained in Short‐Term Tissue Culture

“…These experiments confirm the observations of Basch (1972) that human fetal haemopoietic tissue is erythropoietin responsive. It is now well cstablishcd that in the abscnce of crythropoietin no erythroid-cell differentiation occurs in a human adult bone marrow maintained in vitro (Lajtha, 1952;Berman& Powsner, 1959;Erslev& Hughes, 1960;Astaldi, 1961;Myhre, 1964a, b, c, d ; Wood, 1974a). In the presciit cxpcrimeiits we found that the fetal erythroid cells maintaiiicd without erythropoietin show some degree of differciitiation which followed the same pattern as that of erythropoietin-treated cultures, although at a very low rate.…”

Studies on the Biochemical Defect of Pernicious Anemia. I. In Vitro Observations on Oxygen Consumption, Heme Synthesis and Deoxyribonucleic Acid Synthesis by Pernicious Anemia Bone Marrow12

“…These observa- tions led to the speculation that vitamin B12 might combine with an intrinsic factor or be altered in some way to form a hematopoietic factor in serum (10 (Figure 4). Lajtha observed a rapid loss of proliferative power of marrow cells in suspension cultures in vitro (8). Swan, Reisner, and Silverman have also commented on the progressive decrease in the number of "mitotable" cells in suspension cultures (12).…”

“…A.). Because such studies on patients are difficult to carry out and interpret, precise methods of in vitro assessment of the biochemical activity of P. A. marrow cells are needed.Direct studies on P. A. marrow have been confined, for the most part, to observations of morphologic changes and cell counts in tissue culture (5)(6)(7)(8)(9)(10)(11)(12). In attempting to avoid the difficulties inherent in evaluating these changes in tissue culture and in an effort to secure more direct biochemical information, P. A. marrow has been studied in our laboratory by the following techniques: 1) oxygen consumption, 2) heme synthesis as measured by the rate of incorporation of C14-glycine into heme, and 3) deoxyribonucleic acid (DNA) synthesis by measurement of the rate of incorporation of C14-formate into thymine.…”