Erucic acid evaluation in rapeseed and canola oil by Fourier transform‐infrared spectroscopy

Abstract

A simple and rapid method was developed for the determination of erucic acid in rapeseed oil by single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopy. The various ratios prepared from the three different types of oils (rapeseed, canola, and sunflower) were run on gas chromatograph to identify amount of erucic acid. Therefore, a wide range of erucic acid (0.5-53.0%) was obtained for the calibration. The partial least squares (PLS) calibration was used for predicting erucic acid using spectral region at 2983-657/cm. The root mean square error of prediction (RMSEP), root mean square error of calibration (RMSEC), and root mean square error of cross-validation (RMSECV) were also determined to assess the accuracy of the method. The regression coefficient (R 2 ) was obtained 0.999 with least errors of RMSEC (0.12%), RMSECV (1.96%), and RMSEP (1.38%). The accuracy of the FTIR method was evaluated by analyzing canola and rapeseed oil samples by GC-MS and the results of both the techniques were found to be comparable.Practical applications: Generally, GC is used for determination of erucic acid in vegetable oils. But it needs the very laborious process of derivatization of fatty acids into their respective volatile methyl esters. Furthermore, lot of health hazardous chemicals and reagents are involved in the process of derivatization which are potential source for environmental pollution. Generally, FTIR spectroscopy has been recognized as a very simple, rapid, and cost effective technique. During the last decade, several researchers have used multivariate regression to build up calibration models based on FTIR data. The use of multivariate calibration methods is able to provide, estimates of the precision of the analysis, and yields important qualitative, quantitative, and analytical information. The objective of the present study was to develop a simple methodology for the quantitative analysis of low and high levels of erucic acid in canola and rapeseed oil, respectively by SB-ATR FTIR spectroscopy with PLSs regression.Abbreviations: FAME, fatty acid methyl esters; FTIR, Fourier transform infrared; MDa, mean difference of accuracy; MDr, mean difference of reproducibility; PLS, partial least squares; RMSEC, root mean square error of calibration; RMSECV, root mean square error of cross validation; RMSEP, root mean square error of prediction; SB-ATR, single bounce attenuated total reflectance; SDDa, standard deviation of the differences of accuracy; SDDr, standard deviation of the differences of reproducibility Eur.

Synthesis of Lipase-Immobilized CeO2 Nanorods as Heterogeneous Nano-Biocatalyst for Optimized Biodiesel Production from Eruca sativa Seed Oil

“…Catalysts 2020, 10, x FOR PEER REVIEW 10 of 16 E. sativa oil into biodiesel by disappearance of peaks in E. sativa oil at 1465 and 1095 cm −1 and formation of new peaks at 1435 and 1195 cm −1 in the biodiesel [33]. Similar results have been reported in other research [34].…”

Current Research Developments on the Processing and Improvement of the Nutritional Quality of Rapeseed (Brassica napus L.)

“…The utilization of erucic acid has been explored in many inedible applications such as processing of plasticizers, cosmetics, pharmaceuticals, lubricants, polymers, surfactant, and detergents. Erucic acid is also used in the construction of surface coatings, photographic materials, polyethylene films, nylons, behenic alcohol, behenic acid, and emulsifiers . A growth in the demand for rapeseed rich in erucic acid for industrial targets has led to an interest in developing very high erucic acid rapeseed lines by genetic manipulation.…”

“…Erucic acid is also used in the construction of surface coatings, photographic materials, polyethylene films, nylons, behenic alcohol, behenic acid, and emulsifiers. [74] A growth in the demand for rapeseed rich in erucic acid for industrial targets has led to an interest in developing very high erucic acid rapeseed lines by genetic manipulation. Such an approach is required to overpass the theoretical border of 66% erucic acid level of TAG imposed by the substrate specificity of the acyltransferase which is charge of acylation at position sn-2 of the glycerol moiety of the TAG, which discriminates against VLCFAs.…”

Effect of rapeseed meal supplementation to gestation diet on reproductive performance, blood profiles and milk composition of sows

“…In addition, Gls are associated with a poor performance in reproduction such as delayed sexual maturity, decrease of embryo survival rates, low conception rate and fetal survival rates [ 3 , 8 ]. In the case of erucic acid, high intake is also toxic, and may cause heart damage and lipidosis in cardiac tissues [ 9 ] because it is poorly oxidized by mitochondrial β-oxidation system [ 10 ]. Therefore, the objective of the present study was to evaluate the effect of dietary supplementation levels of RSM on reproductive performance, blood profiles and milk composition of sows and performance of their progeny.…”