Eriodictyol alleviates lipopolysaccharide‐triggered oxidative stress and synaptic dysfunctions in BV‐2 microglial cells and mouse brain

He, Pandi; Yan, Shikai; Wen, Xin; Zhang, Shuhan; Liu, Zhigang; Liu, Xuebo; Xiao, Changlai

doi:10.1002/jcb.28736

Cited by 46 publications

(22 citation statements)

References 60 publications

(132 reference statements)

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“…As it has been shown that LPS induces oxidative stress [ 80 , 81 ], we measured intracellular ROS levels in BV-2 cells pretreated with 50 μ g mL −1 of the extracts for 24 h and then activated by LPS ( Figure 9 ). As expected, LPS significantly increased intracellular ROS levels with respect to controls.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Angeloni

Freschi

Marrazzo

et al. 2021

Oxidative Medicine and Cellular Longevity

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Spent coffee grounds (SCGs), waste products of coffee beverage production, are rich in organic compounds such as phenols. Different studies have demonstrated phenol beneficial effects in counteracting neurodegenerative diseases. These diseases are associated with oxidative stress and neuroinflammation, which initiates the degeneration of neurons by overactivating microglia. Unfortunately, to date, there are no pharmacological therapies to treat these pathologies. The aim of this study was to evaluate the phenolic content of 4 different SCG extracts and their ability to counteract oxidative stress and neuroinflammation. Caffeine and 5-O-caffeoylquinic acid were the most abundant compounds in all extracts, followed by 3-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid. The four extracts demonstrated a different ability to counteract oxidative stress and neuroinflammation in vitro. In particular, the methanol extract was the most effective in protecting neuron-like SH-SY5Y cells against H2O2-induced oxidative stress by upregulating endogenous antioxidant enzymes such as thioredoxin reductase, heme oxygenase 1, NADPH quinone oxidoreductase, and glutathione reductase. The water extract was the most effective in counteracting lipopolysaccharide-induced neuroinflammation in microglial BV-2 cells by strongly reducing the expression of proinflammatory mediators through the modulation of the TLR4/NF-κB pathway. On these bases, SCG extracts could represent valuable nutraceutical sources for the treatment of neurodegeneration.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Angeloni

Freschi

Marrazzo

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Eriodictyol is a natural flavonoid compound. Previous studies have shown that eriodictyol exerts anti-inflammatory and antioxidant effects both in vitro and in vivo (Li et al, 2018;He et al, 2019). However, the effects of eriodictyol on cancer and its underlying mechanisms largely remain unexplored.…”

Section: Discussionmentioning

confidence: 99%

Eriodictyol Inhibits Proliferation, Metastasis and Induces Apoptosis of Glioma Cells via PI3K/Akt/NF-κB Signaling Pathway

et al. 2020

Front. Pharmacol.

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Glioma is the most common type of malignant brain tumor. Due to its highly aggressive and metastatic features, glioma is associated with poor prognosis and a lack of effective treatments. Eriodictyol, a natural flavonoid compound, has been reported to possess antiinflammatory and antioxidant effects. However, the anti-tumor effects of eriodictyol and the underlying mechanisms have rarely been reported. In this study, we found that eriodictyol has anti-tumor activity in lung, colon, breast, pancreas, and liver cancer, and most significantly in glioma cell lines. Eriodictyol dose-and time-dependently suppresses cell proliferation, migration, and invasion in U87MG and CHG-5 glioma cells. In addition, eriodictyol induces apoptosis in U87MG and CHG-5 cells, as evaluated by flow cytometry, immunofluorescence, and Western blot. Furthermore, eriodictyol downregulates the phosphoinositide 3-kinase (PI3K)/Akt/NF-kB signaling pathway in a concentrationdependent manner. Moreover, the effects of eriodictyol on the apoptosis of glioma cells are enhanced by LY294002 (a PI3K inhibitor) and reversed by 740 Y-P (a PI3K agonist). In a mouse xenograft model, eriodictyol not only dramatically suppressed tumor growth but also induced apoptosis in tumor cells. In summary, our data illustrate that eriodictyol effectively inhibits proliferation and metastasis and induces apoptosis of glioma cell lines, which might be a result of the blockade of the PI3K/Akt/NF-kB signaling pathway.

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“…Importantly, accumulating evidences have shown that brain SIRT-1 plays neuroprotective roles in the context of neurodegenerative disorders and cerebral ischemia [ 30 ]. Studies have reported that increased SIRT-1 expression is related to reduced LPS-induced synaptic dysfunctions, suggesting a potential intervention for oxidative stress-related neurodegenerative diseases [ 52 ]. Also, studies have shown that activating SIRT-1 alleviated the SAH injury primarily by inhibiting the TLR4 [ 34 ] and NF- κ B signalling pathway [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Pterostilbene Attenuates Cocultured BV-2 Microglial Inflammation-Mediated SH-SY5Y Neuronal Oxidative Injury via SIRT-1 Signalling

Zhu

Tang

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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Microglial inflammation plays an important part in the progression of multiple neurological diseases, including neurodegenerative diseases, stroke, depression, and traumatic encephalopathy. Here, we aimed to explore the role of pterostilbene (PTE) in the microglial inflammatory response and subsequent damage of cocultured neural cells and partially explain the underlying mechanisms. In the coculture system of lipopolysaccharide-activated BV-2 microglia and SH-SY5Y neuroblastoma, PTE (only given to BV-2) exhibited protection on SH-SY5Y cells, evidenced by improved SH-SY5Y morphology and viability and LDH release. It also attenuated SH-SY5Y apoptosis and oxidative stress, evidenced by TUNEL and DCFH-DA staining, as well as MDA, SOD, and GSH levels. Moreover, PTE upregulated SIRT-1 expression and suppressed acetylation of NF-κB p65 subunit in BV-2 microglia, thus decreasing the inflammatory factors, including TNF-α and IL-6. Furthermore, the effects above were reversed by SIRT-1 inhibitor EX527. These results suggest that PTE reduces the microglia-mediated inflammatory response and alleviates subsequent neuronal apoptosis and oxidative injury via increasing SIRT-1 expression and inhibiting the NF-κB signalling pathway.

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Eriodictyol alleviates lipopolysaccharide‐triggered oxidative stress and synaptic dysfunctions in BV‐2 microglial cells and mouse brain

Cited by 46 publications

References 60 publications

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Eriodictyol Inhibits Proliferation, Metastasis and Induces Apoptosis of Glioma Cells via PI3K/Akt/NF-κB Signaling Pathway

Pterostilbene Attenuates Cocultured BV-2 Microglial Inflammation-Mediated SH-SY5Y Neuronal Oxidative Injury via SIRT-1 Signalling

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