Eriodictyol Inhibits Proliferation, Metastasis and Induces Apoptosis of Glioma Cells via PI3K/Akt/NF-κB Signaling Pathway

Abstract

Glioma is the most common type of malignant brain tumor. Due to its highly aggressive and metastatic features, glioma is associated with poor prognosis and a lack of effective treatments. Eriodictyol, a natural flavonoid compound, has been reported to possess antiinflammatory and antioxidant effects. However, the anti-tumor effects of eriodictyol and the underlying mechanisms have rarely been reported. In this study, we found that eriodictyol has anti-tumor activity in lung, colon, breast, pancreas, and liver cancer, and most significantly in glioma cell lines. Eriodictyol dose-and time-dependently suppresses cell proliferation, migration, and invasion in U87MG and CHG-5 glioma cells. In addition, eriodictyol induces apoptosis in U87MG and CHG-5 cells, as evaluated by flow cytometry, immunofluorescence, and Western blot. Furthermore, eriodictyol downregulates the phosphoinositide 3-kinase (PI3K)/Akt/NF-kB signaling pathway in a concentrationdependent manner. Moreover, the effects of eriodictyol on the apoptosis of glioma cells are enhanced by LY294002 (a PI3K inhibitor) and reversed by 740 Y-P (a PI3K agonist). In a mouse xenograft model, eriodictyol not only dramatically suppressed tumor growth but also induced apoptosis in tumor cells. In summary, our data illustrate that eriodictyol effectively inhibits proliferation and metastasis and induces apoptosis of glioma cell lines, which might be a result of the blockade of the PI3K/Akt/NF-kB signaling pathway.

Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

“…To confirm whether circ‐PRMT5 exerts its effects in breast cancer via the miR‐509‐3p/TCF7L2 axis and the PI3K/AKT pathway, we performed the subsequent rescue assays. 740 Y‐P, a well‐known activator of the PI3K/AKT pathway, was used at a concentration of 20 μg/mL 20,21 . As shown in Figure 6A,B, breast cancer cell viability and proliferation capacity were inhibited by down‐regulated circ‐PRMT5 but restored after overexpressing pcDNA3.1/TCF7L2.…”

COLEC12 regulates apoptosis of osteosarcoma through Toll‐like receptor 4–activated inflammation

“…After 12 hours, various concentrations (0, 25, 50, 100 mmol/L) of eriodyctiol were added to each well, and cells were incubated for an additional 48 hours. Detailed procedures were performed as described in reference 22 …”

Flavonoids in Cancer Metastasis

“…Eriodictyol concentration-dependently inhibited tumor growth and metastasis via the downregulation of the PI3K/Akt/NF-κB signaling pathway in U87MG and CHG-5 glioma cells [ 90 ].…”