Epstein-Barr virus in normal, pre-malignant, and malignant lesions of the uterine cervix.

Landers, R J; O’Leary, John; Crowley, Michael J.; Healy, I; Annis, P; Burke, Louise M.; O’Brien, Dennis P.; Hogan, John M.; Kealy, W F; Lewis, F A

doi:10.1136/jcp.46.10.931

Cited by 63 publications

(49 citation statements)

References 11 publications

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“…These results are comparable with those published by Landers et al (15), who found that 8% of CIN 2 and CIN 3 samples tested positive for EBV DNA. Again, the presence of EBV DNA within the cervix raises the possibility that this virus may be sexually transmitted.…”

Section: Discussionsupporting

confidence: 82%

Detection of Cervical Infections in Colposcopy Clinic Patients

Lanham¹,

Herbert

Basarab

et al. 2001

J Clin Microbiol

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The purpose of this study was to determine if Neisseria gonorrhoeae; Chlamydia trachomatis; herpes simplex virus; cytomegalovirus; Epstein-Barr virus; human herpesviruses 6, 7, and 8; or adeno-associated virus influenced the production of cervical intraepithelial neoplasia. Two hundred thirty-one cervical smear samples were tested for the presence of the organisms by PCR. In addition, human papillomavirus types in the samples were determined by PCR and classified into cancer risk types of high, moderate, and low. There was no link with cervical intraepithelial neoplasia status and detection of herpes simplex virus, cytomegalovirus, EpsteinBarr virus, human herpesviruses 6 and 8, gonorrhea, or chlamydia. However, high-grade cervical intraepithelial neoplasia was found more frequently with mixed infection by moderate-risk human papillomavirus types and human herpesvirus 7 than with these papillomavirus types alone. The presence of human herpesvirus 7 may increase the oncogenic potential of moderate-risk human papillomavirus types.

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Section: Discussionsupporting

confidence: 82%

Detection of Cervical Infections in Colposcopy Clinic Patients

Lanham¹,

Herbert

Basarab

et al. 2001

J Clin Microbiol

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“…These results concur with those found in a recent (18). A similar increase in prevalence of cervical EBV DNA shedding has been reported in other studies as well (11,12,26). Sasagawa et al evaluated EBV mRNA expression at the cervix and found that the EBV genes EBER-1, LMP-1, and EBNA-2 were expressed significantly more frequently in tissue samples from CIN and cancer patients than women with normal cervices (19).…”

Section: Discussionsupporting

confidence: 51%

Shedding of Epstein-Barr Virus and Cytomegalovirus from the Genital Tract of Women in a Periurban Community in Andhra Pradesh, India

et al. 2011

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We found a large number of false-positive readings by visual inspection with acetic acid (VIA) in a study of cervical cancer screening strategies (VIA, human papillomavirus HPV DNA testing, and Pap cytology) in a periurban community in Andhra Pradesh, India. We evaluated whether these false-positive readings might be occurring as a result of infections with Epstein-Barr virus (EBV) or cytomegalovirus (CMV), prevalent latent herpesviruses known to be shed from the female genital tract. While we found that there was no association between VIA results and the presence of EBV or CMV in the cervix, we did find a high prevalence of both viruses: 20% for EBV and 26% for CMV. In multivariate analyses, CMV prevalence was associated with younger age, lack of running water in the home, and visually apparent cervical inflammation. EBV prevalence was associated with older age and a diagnosis of cervical intraepithelial neoplasia grade 1 or greater. The biological and clinical implications of these viruses at the cervix remain to be determined. The strong association between the presence of EBV and cervical disease warrants future exploration to determine whether EBV plays a causal role in disease development or if it is merely a bystander in the process.We conducted a study of cervical cancer screening strategies in a periurban community near Hyderabad in the state of Andhra Pradesh in southern India. Cervical cells were collected from women for cytological and virological investigation, and the cervix was examined with naked eye visual inspection after application of acetic acid (VIA). We found that a positive VIA reading (presence of an acetowhite lesion in the cervical transformation zone) had a surprisingly low sensitivity (26.3%) and moderate specificity (76.4%) for the detection of cervical neoplasia (9,28).Given the large number of false-positive VIA readings, we inquired if these lesions might be occurring as a result of infections with Epstein-Barr virus (EBV) or cytomegalovirus (CMV), prevalent latent herpesviruses which are known to be shed from the female genital tract (2, 6, 14, 21). We also examined whether cervical EBV and CMV detection correlated with either cervical HPV detection or other markers of cervical pathology, since these viruses have been postulated to have a possible cocarcinogenic role with human papillomavirus (HPV) for cervical neoplasia (19, 23). MATERIALS AND METHODSStudy participants. The study subjects were a subpopulation of the CATCH (Community Access to Cervical Health) project, a population-based screening study in a periurban community near Hyderabad, in the state of Andhra Pradesh, India. The CATCH project aimed to enroll all eligible women 25 years of age and older who were not pregnant and who had not had a hysterectomy. A total of 2,331 women, or approximately 40% of those eligible, were enrolled and consented to an interviewer-administered questionnaire to collect data on demographics, reproductive health, cervical cancer screening history, and smoking status. Participants also pr...

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“…Sixbey et al (13) have demonstrated that uterine cervical cells can be infected by EBV, following the findings of Landers et al (14), who found EBV in 43% of cervical cancer tissues, 8% of cervical intraepithelial neoplasias, and 0% of normal cervical tissues.…”

Section: Introductionmentioning

confidence: 99%

Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Santos

Villanova²,

Andrade³

et al. 1994

Oncol Rep

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Abstract. In the present study, our aim was to investigate whether EBV DNA could be found in association with invasive and pre-invasive cervical cancer lesions. We hypothesize that EBV is not merely a commensal agent when present in malignant cervical lesions. DNA was extracted from cervical scrapings followed by nested PCR-based amplification. The patients were 66 women with high grade cervical intraepithelial neoplasia and 14 women with invasive cervical cancer. The control group consisted of 89 women with a normal Pap smear and colposcopy as well as a negative HPV DNA test. Analysis of our results, in conjunction with the work of other authors, leads us to propose that EBV is not merely a commensal agent when present in malignant cervical lesions. The presence of DNA from EBV is significantly associated with cervical cancer.

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Epstein-Barr virus in normal, pre-malignant, and malignant lesions of the uterine cervix.

Cited by 63 publications

References 11 publications

Detection of Cervical Infections in Colposcopy Clinic Patients

Detection of Cervical Infections in Colposcopy Clinic Patients

Shedding of Epstein-Barr Virus and Cytomegalovirus from the Genital Tract of Women in a Periurban Community in Andhra Pradesh, India

Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

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