Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Santos, Nelly Beatriz Modós; Villanova, Fabiola; Andrade, P. M.; Ribalta, Julisa; Focchi, José; Otsuka, Audrey Yumi; Silva, Ismael Dale

doi:10.3892/or_00000236

Cited by 13 publications

(3 citation statements)

References 6 publications

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“…Genital coinfection of these viruses may disrupt the mucosal epithelial barrier (Hebner and Laimins, 2006) and the different viruses may influence each other in many ways, such as viral entry and clearance (Blanco et al, 2020). Consistent with previous reports (Santos et al, 2009;Kienka et al, 2019;Cameron et al, 2020;Joharinia et al, 2020), we revealed a significant association between EBV and HPV coinfection and CIN2+, suggesting that coinfection of EBV and HPV is a potential risk factor in carcinogenesis. While there is a close relationship between EBV infection and HPV-related cervical cancers, the underlying mechanisms remain unclear.…”

Section: Discussionsupporting

confidence: 91%

Role of Epstein-Barr Virus and Human Papillomavirus Coinfection in Cervical Intraepithelial Neoplasia in Chinese Women Living With HIV

Feng

Duan

Gao

et al. 2021

Front. Cell. Infect. Microbiol.

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Given that only a small percentage of human papillomavirus (HPV)-positive women develop cancer, HPV is necessary but insufficient for carcinogenesis. Mucosally transmitted viral cofactors appear to contribute to HPV-related cervical cancer, such as Epstein-Barr virus (EBV), but previous studies have shown inconsistent outcomes. The exact role of EBV in cervical cancer remains unclear, and more studies are needed to determine its involvement. In this study, we describe the prevalence of EBV and HPV coinfection in HIV-positive women and explore how abnormal host immune status induced by viral coinfections modulates epithelial gene expression. We found a significant correlation between EBV-HPV coinfection and the incidence of high-grade cervical intraepithelial neoplasia (CIN2+). RNA sequencing indicated that CIN tissues coinfected with EBV and HPV led to significant changes in the gene expression of epithelial differentiation and development compared to normal tissues with HPV infection alone. In particular, several differentially expressed genes (DEGs) are closely associated with cancer, such as CACNG4, which was confirmed to be upregulated at both the mRNA and protein levels. Therefore, these findings provide some evidence that EBV may act as a cofactor or mediator in HPV-related cervical cancer. Specific genes or proteins, such as CACNG4, may serve as biomarkers that can risk stratify patients based on pathological changes in the cervix.

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Section: Discussionsupporting

confidence: 91%

Role of Epstein-Barr Virus and Human Papillomavirus Coinfection in Cervical Intraepithelial Neoplasia in Chinese Women Living With HIV

Feng

Duan

Gao

et al. 2021

Front. Cell. Infect. Microbiol.

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“…In one study from Thailand, the EBV genome was found in 13.4% of normal cervical samples, 29.4% of low-grade squamous intraepithelial lesions (LSILs), 49.4% of high-grade squamous intraepithelial lesions (HSIL), and 35% of cervical cancer samples [ 23 ]. In a study from Brazil, the EBV genome was detected in 8.99%, 21.2%, and 64.3% of normal, premalignant, and malignant cervical samples, respectively [ 29 ]. In a study from Portugal, the EBV genome was found in 16.7% of normal samples, 9.5% of cervical intraepithelial neoplasia (CIN 1), 4.5% of CIN 2/3, and 22.2% of cervical cancer [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

The Prevalence of Epstein-Barr Virus in Normal, Premalignant, and Malignant Uterine Cervical Samples in Iran

Chavoshpour-Mamaghani,

Shoja,

Jalilvand

2024

Intervirology

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Introduction: It is suggested that Epstein-Barr virus (EBV) may play an important role in cervical cancer development. Most studies found a higher rate of EBV in cervical cancer samples in comparison to premalignant and normal groups. In this regard, this study aimed to investigate the prevalence of EBV in cervical samples. Methods: In total, 364 samples from 179 healthy subjects, 124 women with premalignant lesions, and 61 patients with cervical cancer were investigated using nested PCR. Results: The mean age ± SE was 54.1 ± 13.4 in women with cervical cancer, 36.1 ± 9.4 among women with premalignant lesions and 36.6 ± 11.5 in healthy individuals. In total, 290 out of 364 samples were HPV positive and the following HPV genotypes were detected among them: HPV 16/18 was found in 43.1%, 23.9%, and 65.5%of normal, premalignant, and malignant samples, respectively, and other high-risk types were detected in 56.9% of normal, 76.1% of premalignant, and 34.5% of malignant samples. The prevalence of EBV was found to be 9.8%, 2.4%, and 2.8% in cervical cancer, premalignant lesions, and normal specimens, respectively, and the difference was statistically significant (P=0.028). The overall frequency of co-infection between EBV and HPV was shown to be 3.6%. The co-infection was more prevalent among HPV 16/18-infected samples than other high-risk HPVs (6.6% vs. 2.9%) although the difference was not reached a statistically significant difference (P=0.23). Conclusion: Our findings indicated that EBV could play an important role as a cofactor in the progression of cervical cancer. However, future studies with larger sample sizes and the expression analysis of EBV transcripts or proteins are mandatory.

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“…The interaction between EBV LMP1 and HPV viral proteins altered NF-κB and MAPK signaling, among others, in a murine model [207], while another study highlighted the necessity of HPV viral oncoproteins E6 and E7 in EBV lytic replication in oral keratinocytes [208]. A helper role of EBV in cervical cancer development has been suggested by several studies due to the presence of both HPV and EBV DNA sequences in malignant tissues [209][210][211][212][213][214][215][216].…”

Section: Other Co-infectionsmentioning

confidence: 99%

The Impact of Co-Infections for Human Gammaherpesvirus Infection and Associated Pathologies

Chinna,

Bratl,

Lambarey

et al. 2023

IJMS

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The two oncogenic human gammaherpesviruses Epstein-Barr virus (EBV) and Kaposi’s sarcoma-associated herpesvirus (KSHV) cause significant disease burden, particularly in immunosuppressed individuals. Both viruses display latent and lytic phases of their life cycle with different outcomes for their associated pathologies. The high prevalence of infectious diseases in Sub-Saharan Africa (SSA), particularly HIV/AIDS, tuberculosis, malaria, and more recently, COVID-19, as well as their associated inflammatory responses, could potentially impact either virus’ infectious course. However, acute or lytically active EBV and/or KSHV infections often present with symptoms mimicking these predominant diseases leading to misdiagnosis or underdiagnosis of oncogenic herpesvirus-associated pathologies. EBV and/or KSHV infections are generally acquired early in life and remain latent until lytic reactivation is triggered by various stimuli. This review summarizes known associations between infectious agents prevalent in SSA and underlying EBV and/or KSHV infection. While presenting an overview of both viruses’ biphasic life cycles, this review aims to highlight the importance of co-infections in the correct identification of risk factors for and diagnoses of EBV- and/or KSHV-associated pathologies, particularly in SSA, where both oncogenic herpesviruses as well as other infectious agents are highly pervasive and can lead to substantial morbidity and mortality.

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Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix

Cited by 13 publications

References 6 publications

Role of Epstein-Barr Virus and Human Papillomavirus Coinfection in Cervical Intraepithelial Neoplasia in Chinese Women Living With HIV

Role of Epstein-Barr Virus and Human Papillomavirus Coinfection in Cervical Intraepithelial Neoplasia in Chinese Women Living With HIV

The Prevalence of Epstein-Barr Virus in Normal, Premalignant, and Malignant Uterine Cervical Samples in Iran

The Impact of Co-Infections for Human Gammaherpesvirus Infection and Associated Pathologies

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