Epstein-Barr Virus and Breast Carcinoma in Iran

Aghdam, Maryam Kazemi; Nadji, Seyed Alireza; Khoddami, Maliheh; Dezfuli, Hoda Baghizadeh; Khademi, Yassaman

doi:10.5812/jjm.12800

Cited by 5 publications

(4 citation statements)

References 16 publications

(19 reference statements)

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“…For EBV, the screening of the viral genome did not show any correlation with the case and control groups (P = 0.164) ( Table 3). The rate of EBV infection in breast cancer patients was not unexpected, as the virus has been detected in many studies to be absent or be at a low rate at this site (45)(46)(47). However, we did not observe any significant association between breast cancer and EBV presence.…”

Section: Discussioncontrasting

confidence: 48%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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Background: The DNA oncoviruses, including human papillomavirus (HPV) and Epstein-Barr virus (EBV) are among the most important infectious agents involved in breast carcinogenesis. These oncoviruses have a broad disrupting effect on cellular miRNAs and their functions, by which they contribute to carcinogenesis. Objectives: In this investigation, we evaluated the correlation between HPV and EBV and the expression level of tumor suppressor miRNAs (miR-143 and 145), clinical outcomes, and their association with stimulating inflammatory cytokines in patients with breast carcinoma. Methods: In our case-control study, 35 cancerous tissues and 35 adjacent non-cancerous tissues were collected from 35 patients. Nested-PCR was set up for the detection of HPV and EBV genomes, and RT-qPCR was used for miRNA expression in the case and control groups. In addition, serum specimens were obtained from all patients (n = 35) and healthy controls (n = 35) to determine the IL-8 serum concentration. Results: We found HPV and EBV in 14.2% (10/70) and 7% (5/70) of all samples, respectively. The distributions of positive samples in the case and control groups were 25.7% (9/35) and 2.9% (1/35) (P = 0.006) for HPV and 11.4% (4/35) and 2.9% (1/35) (P = 0.164) for EBV, respectively. Besides, RT-qPCR showed that miR-143 and miR-145 were significantly downregulated in HPV and EBV-infected cases compared to non-infected ones (P < 0.05). Data also indicated that the promotion of metastasis status was related to miR-143/145 downregulation and HPV infection (P = 0.003). No significant difference was found in serum IL-8 concentration concerning viral infections. Conclusions: Our results suggested the possible involvement of viral infections in breast carcinogenesis and adverse clinical outcomes by downregulating miR-143/145.

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Section: Discussioncontrasting

confidence: 48%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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“…Overall, 6.8% (7/102) of samples comprised 11.76% (6/51) in malignant tissues and 1.9% (1/51) in adjacent non-malignant tissues found to be positive for presence of EBV genome, which was statistically significant (P<0.05).Similar to our results, several case-control reports from Iran and other parts of the world have demonstrated higher prevalence of EBV in malignant tumors as compared with control groups. In several studies, the prevalence rate of EBV in malignant breast tissues and nonmalignant control groups were as follows: 9.3% and 0% in a study by Aghdam et al (Iran, 2017), 8% and 0% in a study by Malekpour et al (Iran, 2015), 5.1% and 0% in a study by Saeedi et al (Iran, 2018), 10% and 0% in a study by Antonsson et al (Australia, 2012), 13.6% and 4.5% in a study by Ladera et al (Venezuela, 2017), respectively (28)(29)(30)(31)(32). It is noteworthy that despite other investigations, our study used adjacent non-malignant tissues to improve the accuracy of viral causality in the development of malignancy.…”

Section: Discussionmentioning

confidence: 88%

MiRNA-218 Is Frequently Downregulated in Malignant Breast Tumors: A Footprint of Epstein-Barr Virus Infection

et al. 2021

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Scan to discover onlineBackground & Objective: The role of Epstein-Barr Virus in development of breast cancer is frequently studied. In this regard, miRNAs are among the contributing elements in the molecular pathophysiology of EBV-related diseases. In addition, a growing number of host miRNAs are believed to be implicated in pathogenesis of breast cancer. MiR-218 is a tumor suppressive miRNA that is subjected to dysregulation in various EBV-associated cancers. We aimed to investigate the frequency of EBV and its relationship with expression status of tumor suppressive miR-218 in breast cancer and adjacent normal tissue.Methods: A total number of 51 fresh malignant breast cancer tissues (cases) and their adjacent normal tissues (controls) were collected. Nested-PCR and RT-qPCR were set to identify EBV frequency and miR-218 expression in cases and controls, respectively.Results: Out of all samples, 6.8% (7/102) comprising 11.6% (6/51) in malignant tissues and 1.9% (1/51) in normal control tissues were positive for EBV (P<0.05). Quantitative data showed that miR-218 was significantly downregulated in malignant tissues compared to control tissues (P<0.0001). In addition, reduced expression of miR-218 was associated with adverse clinical outcomes, metastasis, and higher grades of malignancy. Given the presence of EBV, lower expression of miR-218 was observed in breast cancer group in comparison with normal group (P<0.05). Conclusion:Our results raise the possibility of the relation between EBV infection and miR-218 downregulation in breast cancer and propose further investigations in this regard.

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“…They suggested that EBV may have an etiologic role in breast cancer in Iran. 39 Joshi et al applied Immunohistochemistry and commercial enzyme-linked Immunosorbent assay (ELISA) kit and showed EBNA-1 expression in a significant proportion of breast cancer tissues from rural India. These patients also have a higher immunological response against EBNA-1 33.…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between EBV Virus and Breast Cancer in Khuzestan Province of Iran

Saeedi

Hadi

Hejazi

et al. 2018

jabr

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Breast cancer is the most frequently diagnosed cancer among women worldwide. 1 The incidence of breast cancer in Iran is about 20 new cases per 100 000 women-years. 2 This cancer often leads to complete removal of breast tissue, chemotherapy, radiotherapy and hormonotherapy. 3 Several internal and external factors contribute to the development of this cancer. Internal factors such as age, hormonal effects, lifestyle, obesity, alcohol consumption, smoking, gender, anxiety and stress, genetic predisposition (mutation in BRCA1, 2 and other genes) and family history of breast cancer. 4,5 Exogenous factors include infection with oncogenic viruses such as mouse mammary tumor virus (MMTV), human papilloma virus (HPV) and Epstein-Barr virus (EBV). Oncogenic viruses are contributing to 20% of human cancers. 6 EBV is a human gamma-1 herpes virus which has a double-stranded DNA genome comprised of approximately 170-kilobases that codes more of 85 genes, belonging to the c herpes virus family. 7,8 EBV is mostly transmitted from the host to another host via saliva and infects over 90% of the world population and remains in the body for life. 9 Breast epithelial cells can be infected with EBV by cell to cell contact. 10 This virus is accepted as a major contributor to 20% Burkitt lymphoma, 50% Hodgkin′s lymphoma, 10% stomach carcinomas and almost all endemic nasopharyngeal carcinoma. 11

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Epstein-Barr Virus and Breast Carcinoma in Iran

Cited by 5 publications

References 16 publications

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

MiRNA-218 Is Frequently Downregulated in Malignant Breast Tumors: A Footprint of Epstein-Barr Virus Infection

The Relationship Between EBV Virus and Breast Cancer in Khuzestan Province of Iran

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