EPR Study of Iron Ion Complexes in Human Blood

Krzyminiewski, Ryszard; Kruczyński, Z.; Dobosz, Bernadeta; Zając, Andrzej; Maćkiewicz, Andrzej; Leporowska, Ewa; Folwaczna, Sandra

doi:10.1007/s00723-011-0219-3

Cited by 27 publications

(11 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The g′ = 4.3 EPR band was found in every measured sample. This signal has been often ascribed in literature to non-heme high-spin Fe(III) in a system of rhombic symmetry 57 , 58 . We exclude the possibility that this signal is due to sample environment contamination since it was absent when either the empty cavity or a test tube containing the fixating solution were studied.…”

Section: Discussionmentioning

confidence: 78%

Quantitative comparison of different iron forms in the temporal cortex of Alzheimer patients and control subjects

Bulk

Weerd

Breimer

et al. 2018

Sci Rep

View full text Add to dashboard Cite

We present a quantitative study of different molecular iron forms found in the temporal cortex of Alzheimer (AD) patients. Applying the methodology we developed in our previous work, we quantify the concentrations of non-heme Fe(III) by Electron Paramagnetic Resonance (EPR), magnetite/maghemite and ferrihydrite by SQUID magnetometry, together with the MRI transverse relaxation rate , to obtain a systematic view of molecular iron in the temporal cortex. Significantly higher values of , a larger concentration of ferrihydrite, and a larger magnetic moment of magnetite/maghemite particles are found in the brain of AD patients. Moreover, we found correlations between the concentration of the iron detected by EPR, the concentration of the ferrihydrite mineral and the average iron loading of ferritin. We discuss these findings in the framework of iron dis-homeostasis, which has been proposed to occur in the brain of AD patients.

show abstract

Section: Discussionmentioning

confidence: 78%

Quantitative comparison of different iron forms in the temporal cortex of Alzheimer patients and control subjects

Bulk

Weerd

Breimer

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The literature values of g factors for three components are g ≈ 4.39; g ≈ 4.19 and g ≈ 4.07, respectively (Rottman, Doi, Zak, Aasa & Aisen, 1989). Two nonheme Fe 3+ ions are in the high-spin state S = 5/2 and their EPR spectrum is characteristic of the compounds in which iron is present in the rhombic symmetry system (Krzyminiewski, Kruczyński, Dobosz, Zając, Mackiewicz, Leporowska & Folwaczna, 2011;Preoteasa, Fig. 2.…”

Section: Resultsmentioning

confidence: 95%

EPR Study of Paramagnetic Centers in Human Blood

Kubiak

Krzyminiewski

Dobosz

2013

Current Topics in Biophysics

Self Cite

View full text Add to dashboard Cite

Electron paramagnetic resonance investigations of paramagnetic centers in whole human blood were carried out at 170 K using X-band EPR spectrometer. The study included a group of patients and healthy volunteers. The EPR signals from high spin Fe3+ ions in transferrin (g = 4.2) and Cu2+ ions in ceruloplasmin (g = 2.05) are characteristic of each frozen blood sample. An overview of all recorded spectra revealed in several cases additional lines derived from high spin Fe3+ ions in methemoglobin (g = 5.8 - 6), free radicals (g = 2.002 - 2.005) and various low spin ferriheme complexes (g = 2.21 - 2.91). The lines from cytochromes (g = 3.03 and 3.27) were observed only twice. The EPR measurements have not confirmed the correlation between the occurrence of a particular type of low-spin iron complex and a specific disease entity. Moreover, the presence of EPR lines from trivalent iron also did not differentiate patients from healthy volunteers.

show abstract

“…Comparison with the literature shows that this signal originates from Fe 3+ in transferrin ( g = 4.2) (Hirota et al 2000 ; Kubiak et al 2013 ; Pocklington and Foster 1977 ), which is also confirmed by the very characteristic shape. Two non-heme iron atoms are in the high-spin state S = 5/2 and their EPR spectrum is characteristic of the compounds in which iron is present in the rhombic symmetry system (Krzyminiewski et al 2011 ; Pinkowitz and Aisen 1972 ) in an octahedral surrounding (Krzyminiewski et al 2011 ). Because of the complexity of this signal, it has been recorded in the magnetic field range of 50 mT, which is shown in Figs.…”

Section: Resultsmentioning

confidence: 99%

“…Electron paramagnetic resonance spectroscopy is an ideal tool for the study of paramagnetic centers in human blood (Krzyminiewski et al 2011 ; Kubiak et al 2013 ). The centers include primarily the molecular complexes containing iron Fe 3+ (transferrin, methemoglobin) or copper Cu 2+ ions (ceruloplasmin).…”

Section: Introductionmentioning

confidence: 99%

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Krzyminiewski

Dobosz

Kubiak

2017

Radiat Environ Biophys

Self Cite

View full text Add to dashboard Cite

Ceruloplasmin and transferrin are proteins which play a potential role in the process of breast cancer development. These molecules contain Cu2+ (ceruloplasmin) or Fe3+ ions (transferrin) and thus constitute paramagnetic centers, which can be studied using electron paramagnetic resonance method. The aim of the study was to determine how paramagnetic centers in whole blood of breast cancer patients change under the influence of radiation therapy. Samples of whole blood were taken from 17 women with breast cancer treated with radiotherapy. The measurements were carried out at 170 K using X-band electron paramagnetic resonance (EPR) spectrometer Bruker EMX-10. Two distinct EPR lines, derived from high-spin Fe3+ in transferrin and Cu2+ from ceruloplasmin, were revealed in all frozen samples. The amplitude and integrated intensity of the EPR signal from Cu2+ in ceruloplasmin significantly decreased in all patients after the delivery of the radiation fraction. When comparing the integral intensity of the signal from Fe3+ in transferrin, three different situations were identified which are patient specific: a significant increase, an insignificant change, or a significant decrease after the irradiation. A decreased level of Cu2+ from ceruloplasmin in patients after radiotherapy means a low level of ceruloplasmin in the plasma or an increased content of reduced Cu+ ions. Differences in the integrated intensity of the EPR signal from transferrin translate directly into the amount of bound iron. The observed changes could indicate how well the organism fights against cancer and how easily it adapts to the situation of biochemical stress.

show abstract

EPR Study of Iron Ion Complexes in Human Blood

Cited by 27 publications

References 16 publications

Quantitative comparison of different iron forms in the temporal cortex of Alzheimer patients and control subjects

Quantitative comparison of different iron forms in the temporal cortex of Alzheimer patients and control subjects

EPR Study of Paramagnetic Centers in Human Blood

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Contact Info

Product

Resources

About