EPR Study of Paramagnetic Centers in Human Blood

Kubiak, Tomasz; Krzyminiewski, Ryszard; Dobosz, Bernadeta

doi:10.2478/ctb-2013-0006

Cited by 19 publications

(8 citation statements)

References 28 publications

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“…The g factor, assigned to the line formed by the merger of two components with highest amplitudes, is in the g range of 4.14–4.16. Comparison with the literature shows that this signal originates from Fe 3+ in transferrin ( g = 4.2) (Hirota et al 2000 ; Kubiak et al 2013 ; Pocklington and Foster 1977 ), which is also confirmed by the very characteristic shape. Two non-heme iron atoms are in the high-spin state S = 5/2 and their EPR spectrum is characteristic of the compounds in which iron is present in the rhombic symmetry system (Krzyminiewski et al 2011 ; Pinkowitz and Aisen 1972 ) in an octahedral surrounding (Krzyminiewski et al 2011 ).…”

Section: Resultssupporting

confidence: 64%

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The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Krzyminiewski

Dobosz

Kubiak

2017

Radiat Environ Biophys

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Ceruloplasmin and transferrin are proteins which play a potential role in the process of breast cancer development. These molecules contain Cu2+ (ceruloplasmin) or Fe3+ ions (transferrin) and thus constitute paramagnetic centers, which can be studied using electron paramagnetic resonance method. The aim of the study was to determine how paramagnetic centers in whole blood of breast cancer patients change under the influence of radiation therapy. Samples of whole blood were taken from 17 women with breast cancer treated with radiotherapy. The measurements were carried out at 170 K using X-band electron paramagnetic resonance (EPR) spectrometer Bruker EMX-10. Two distinct EPR lines, derived from high-spin Fe3+ in transferrin and Cu2+ from ceruloplasmin, were revealed in all frozen samples. The amplitude and integrated intensity of the EPR signal from Cu2+ in ceruloplasmin significantly decreased in all patients after the delivery of the radiation fraction. When comparing the integral intensity of the signal from Fe3+ in transferrin, three different situations were identified which are patient specific: a significant increase, an insignificant change, or a significant decrease after the irradiation. A decreased level of Cu2+ from ceruloplasmin in patients after radiotherapy means a low level of ceruloplasmin in the plasma or an increased content of reduced Cu+ ions. Differences in the integrated intensity of the EPR signal from transferrin translate directly into the amount of bound iron. The observed changes could indicate how well the organism fights against cancer and how easily it adapts to the situation of biochemical stress.

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Section: Resultssupporting

confidence: 64%

“…Electron paramagnetic resonance spectroscopy is an ideal tool for the study of paramagnetic centers in human blood (Krzyminiewski et al 2011 ; Kubiak et al 2013 ). The centers include primarily the molecular complexes containing iron Fe 3+ (transferrin, methemoglobin) or copper Cu 2+ ions (ceruloplasmin).…”

Section: Introductionmentioning

confidence: 99%

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Krzyminiewski

Dobosz

Kubiak

2017

Radiat Environ Biophys

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“…Indeed, the g′ = 5.8 band, commonly associated with Fe(III)- heme iron in methemoglobin, suggests that the residual blood57 in the tissue is a minor fraction and it can be neglected in the spectral simulation, due to its different g′ value. Transferrin, a possible candidate for the band observed here40 has a typical linewidth of ~125 G pp at 12 K and a splitting of the g′ = 4.3 peak of ~32 G434457. In contrast, the g′ = 4.3 band observed has a width of 57 ± 2 G pp across all temperatures, and no splitting.…”

Section: Discussionmentioning

confidence: 99%

A novel approach to quantify different iron forms in ex-vivo human brain tissue

Kumar

Bulk

Webb

et al. 2016

Sci Rep

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We propose a novel combination of methods to study the physical properties of ferric ions and iron-oxide nanoparticles in post-mortem human brain, based on the combination of Electron Paramagnetic Resonance (EPR) and SQUID magnetometry. By means of EPR, we derive the concentration of the low molecular weight iron pool, as well as the product of its electron spin relaxation times. Additionally, by SQUID magnetometry we identify iron mineralization products ascribable to a magnetite/maghemite phase and a ferrihydrite (ferritin) phase. We further derive the concentration of magnetite/maghemite and of ferritin nanoparticles. To test out the new combined methodology, we studied brain tissue of an Alzheimer’s patient and a healthy control. Finally, we estimate that the size of the magnetite/maghemite nanoparticles, whose magnetic moments are blocked at room temperature, exceeds 40–50 nm, which is not compatible with the ferritin protein, the core of which is typically 6–8 nm. We believe that this methodology could be beneficial in the study of neurodegenerative diseases such as Alzheimer’s Disease which are characterized by abnormal iron accumulation in the brain.

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“…MAENHAUT; DE REUCK, 1989;HALLGREN;SOURANDER, 1958;KREBS et al, 2014). DOBOSZ, 2013;SHIMA et al, 1997;ZECCA et al, 1996); g = 4,28…”

Section: Solução De Ferritinamentioning

confidence: 99%

Conteúdo de íons paramagnéticos no cérebro e sua correlação com mapas quantitativos de ressonância magnética

Barbosa¹

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Ao Prof. Dr. Carlos Garrido, agradeço por ter me orientado como aluno de mestrado e agora como doutorado. Agradeço todos os ensinamentos prestados e a paciência, para que durante o doutorado eu pudesse aprender o máximo do conhecimento a minha volta. Agradeço por ter confiado e permitido explorar as fronteiras do conhecimento. Ao Prof. Dr. Baffa, agradeço pela imensa atenção e discussão referente aos resultados de ressonância paramagnética eletrônica e de todos os outros que evolveram essa tese, incluindo o meu crescimento científico. Ao Prof. Dr. Fernando Barbosa, agradeço pela realização e discussão referente aos experimentos de espectrometria de massa.

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EPR Study of Paramagnetic Centers in Human Blood

Cited by 19 publications

References 28 publications

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

A novel approach to quantify different iron forms in ex-vivo human brain tissue

Conteúdo de íons paramagnéticos no cérebro e sua correlação com mapas quantitativos de ressonância magnética

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