Epizootiology, Clinical Signs, and Phylogenetic Analysis of Fowl Adenovirus in Chicken Farms in Indonesia from 2018 to 2019

Wibowo, Michael Haryadi; Sahesty, Aprilla; Mahardika, Bayu K.; Purwanto, Budi; Lestariningsih, Christina Lilis; Suardana, Ida Bagus Kade; Winaya, Ida Bagus Oka; Irine, Ine; Suryanggono, Jodi; Jonas, Melina; Murwijati, Theresia; Mahardika, Gusti Ngurah

doi:10.1637/aviandiseases-d-19-00127

Cited by 6 publications

(11 citation statements)

References 37 publications

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“…Data from the results of the present study have not been able to determine the source of spread nor the origin of FAdV infections in Indonesia. Wibowo et al [8] reported that the highest number of FAdV cases in Indonesia between 2018 and 2019 came from East Kalimantan, East Java, and South Kalimantan, where the species that were infected are the same as FAdV found in the present study with homology 98.9-100%. From these results, we conclude that the [8].…”

Section: Discussionsupporting

confidence: 66%

“…Wibowo et al [8] reported that the highest number of FAdV cases in Indonesia between 2018 and 2019 came from East Kalimantan, East Java, and South Kalimantan, where the species that were infected are the same as FAdV found in the present study with homology 98.9-100%. From these results, we conclude that the [8]. Similar reports regarding the source of the FAdV spread also described in Japan by Mase and Nakamura [21].…”

Section: Discussionsupporting

confidence: 66%

“…FAdV infection has been reported in various countries, most cases of FAdV attack broiler chickens aged between 2 and 5 weeks [ 7 , 8 , 15 - 17 ]; however, FAdV infections in layers and breeders are not uncommon [ 3 , 18 ]. The results of this study did not distinguish observations and final confirmation between types of broiler, layer, or breeder farms.…”

Section: Discussionmentioning

confidence: 99%

“…The investigation by Wibowo et al . shows that the spread of FAdV occurs without a clear epizootiological relation [ 8 ]. Similar reports regarding the source of the FAdV spread also described in Japan by Mase and Nakamura [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Over the past few years, there have been complaints of the presence of IBH or hepatitis hydropericardium syndrome in Indonesia. The outbreak of the syndrome started in 2018 [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia

et al. 2020

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Background and Aim: Fowl avidenoviruses (FAdVs) are generally considered ubiquitous, but certain serotypes and strains are known to be associated with primary diseases, such as inclusion body hepatitis (IBH). Since 2018, the outbreak of IBH has been reported in part provinces of Indonesia. This study aimed to isolate and molecularly characterize the FAdV from Banten and West Java Provinces of Indonesia and described the phylogenetic relationship with the FAdV that has been characterized in other countries. Materials and Methods: A total of 25 FAdV archive samples have been collected from January to August 2019 from clinical cases of FAdV infection in Banten and West Java Provinces, Indonesia. Collected samples were inoculated in 10-day-old specific-pathogenic-free chicken embryonated eggs. Hexon gene of FAdV was detected using polymerase chain reaction (PCR) with a primer set from previous study. To gain a better understanding of the FAdV genetic properties and construct the phylogeny tree, the PCR products were sequenced and subjected to a BLAST search and inferred using the neighbor-joining method by bootstrap test 1000×. Results: FAdV-D and FAdV-E are present in Banten, Indonesia. The phylogenetic analysis of 850 nucleotides that encode 289 amino acid of the partial hexon gene shows that the isolates Broiler/MSL/Ciputat-149/18, Broiler/MSL/Lebak-151/18, and Broiler/MSL/Ciputat-29/19 have 100% homology with FAdV-E TR/BVKE/R/D-1 from Turkey, whereas the isolates Layer/MSL/Ciputat-20/19 and Broiler/MSL/Ciputat-30/19 have 100% homology with FAdV-D strain 685 from Canada. Conclusion: The present study provides updates of the circulating FAdV in commercial poultry flocks in Banten and West Java Provinces, Indonesia. Since the FAdV vaccine was unavailable in Indonesia, this result might be used as guidance to select a proper FAdV vaccine strain. Our result indicates that at least two FAdV species were circulating among poultry in Banten and West Java Provinces, Indonesia; they are FAdV-D and FAdV-E.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Over the past few years, there have been complaints of the presence of IBH or hepatitis hydropericardium syndrome in Indonesia. The outbreak of the syndrome started in 2018 [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia

et al. 2020

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A 24-Year-Old Sample Contributes the Complete Genome Sequence of Fowl Aviadenovirus D from the United States

Goraichuk

Davis

Kulkarni

et al. 2021

Microbiol Resour Announc

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First Evidence of Fowl Adenovirus Induced Inclusion Body Hepatitis in Chicken in Bangladesh

Islam

Rahman

et al. 2023

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. The livestock sector contributes 1.90% to the GDP in Bangladesh during 2021–22. Poultry is one of the important subsectors struggling with diseases. Fowl adenoviruses (FAdVs) cause numerous diseases resulting in economic losses to the poultry industry worldwide. Several FAdV serotypes cause inclusion body hepatitis in chicken. Although FAdV infection was suspected, there was no confirmatory report from Bangladesh. The study was conducted to investigate the FAdV infection and antibodies in chicken. Methods. A total of 50 samples, each composed of liver and spleen, were collected from different chickens of Gazipur, Dinajpur, and Panchagarh district. Each location belongs to A, B, and C poultry zones of Bangladesh, respectively. Viruses were detected by real-time PCR and conventional PCR. Blood samples (n = 303) were collected at the beginning and after the recovery from infection and tested by indirect ELISA. Sequencing of PCR products was done for serotyping and phylogenetic analysis. Results. Clinical signs were observed including anorexia, drowsiness, ruffled feathers, reduced body weight, lack of uniformity, and high mortality (15–25%). Enlarged friable liver with yellow to tan color mottled with the focal soft area, fluid in pericardial sac, swollen and hemorrhagic kidneys, enlarged congested spleen and pancreas, etc. were found on postmortem examination. FAdVs were detected in 90% of the flocks except commercial layer flock from Dinajpur. Three serotypes, namely, 8b (70%), 11 (10%), and 5 (10%) were detected. Anti-FAdV antibody was detected in 80% flocks at the beginning of infection and in 90% of the flocks after recovery from infection. The antibody titer increases significantly ( p < 0.05 ) after recovery from infection. Phylogenetic analysis revealed that the Bangladeshi FAdVs have close identity with viruses from Asia, Europe, and South and North America. Conclusions. These findings suggested that several introductions of FAdVs were taken place in Bangladesh. To combat the disease, vaccination along with maintenance of biosecurity is essential.

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Epizootiology, Clinical Signs, and Phylogenetic Analysis of Fowl Adenovirus in Chicken Farms in Indonesia from 2018 to 2019

Cited by 6 publications

References 37 publications

Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia

Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia

A 24-Year-Old Sample Contributes the Complete Genome Sequence of Fowl Aviadenovirus D from the United States

First Evidence of Fowl Adenovirus Induced Inclusion Body Hepatitis in Chicken in Bangladesh

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