Epithelial–mesenchymal transition in serous and mucinous epithelial tumors of the ovary

Bhuyan, Geet; Arora, Rashmi; Ahluwalia, Charanjeet; Sharma, Preeti

doi:10.4103/jcrt.jcrt_35_18

Cited by 13 publications

(7 citation statements)

References 2 publications

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“…Vimentin is a typical marker of mesenchymal cells that is involved in several aspects of tumorigenesis and progression, including tumor initiation, EMT, and metastasis of tumor cells (Wu et al 2019). Furthermore, vimentin affects cell-cell and cell-extracellular matrix (ECM) adhesion and thus is considered a key regulator of cell movement (Bhuyan et al 2019). The results of the present study demonstrated that E-cadherin expression decreased and vimentin expression increased in the TGF-β group, suggesting that the EMT model induced by TGF-β in A549 cells was successful in vitro.…”

Section: Discussionmentioning

confidence: 51%

Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells

Shi

Zhai

et al. 2021

Cell Stress and Chaperones

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Heat shock protein 70 (HSP70) is a key member of the HSP family that contributes to a pre-cancerous environment; however, its role in lung cancer remains poorly understood. The present study used geranylgeranylacetone (GGA) to induce HSP70 expression, and transforming growth factor-β (TGF-β) was used to construct an epithelial-mesenchymal transition (EMT) model by stimulating A549 cells in vitro. Western Blot was performed to detect protein levels of NADPH oxidase 4 (NOX4) and the EMTassociated proteins E-cadherin and vimentin both before and after HSP70 expression. Cell morphological changes were observed, and the effect of HSP70 on cell migration ability was detected via the wound healing. The results demonstrated that GGA at 50 and 200 μmol/L could significantly induce HSP70 expression in A549 cells (P < 0.05). Furthermore, HSP70 induced by 200 μmol/L GGA significantly inhibited the changes of E-cadherin, vimentin, and cell morphology induced by TGF-β (P < 0.05), while HSP70 induced by 50 μmol/L GGA did not. The results of the wound healing assay indicated that 200 μmol/L GGA significantly inhibited A549 cell migration induced by TGF-β. Taken together, the results of the present study demonstrated that overexpression of HSP70 inhibited the TGF-β induced EMT process and changed the cell morphology and migratory ability induced by TGF-β in A549 cells.

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Section: Discussionmentioning

confidence: 51%

Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells

Shi

Zhai

et al. 2021

Cell Stress and Chaperones

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“…e loss of E-cadherin promotes the release of β-catenin and thus promotes EMT, while the expression of E-cadherin can reverse the transformed phenotype [43][44][45][46], and thus the loss of cell-cell adhesion triggers EMT and is related to diseases involving EMT [41]. Studies have shown that inhibiting the expression of cell adhesion molecules (such as E-cadherin) and mesenchymal markers (such as vimentin) is a key process in EMT, while the positive EMT state (decreased E-cadherin expression) is a primary feature of OC and metastasis [47,48].…”

Section: The Wnt/β-catenin Signaling Pathway In Ocmentioning

confidence: 99%

Plant-Derived Chinese Medicine Monomers on Ovarian Cancer via the Wnt/β-Catenin Signaling Pathway: Review of Mechanisms and Prospects

Liu

et al. 2021

Journal of Oncology

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Ovarian cancer (OC) is a common malignant tumor of the female reproductive system and has a high morbidity and mortality rate. The progression and metastasis of OC are complex and involve multiple signaling pathways. The Wnt/β-catenin signaling pathway is closely related to OC, and therefore blocking the activation of the Wnt/β-catenin signaling directly or inhibiting related genes, and molecular targets is of great value in treating OC. Toxicities such as myelotoxicity, cardiotoxicity, genotoxicity, and vasospasm are the major side effects for common anticancer drugs and are well documented. There is, therefore, a need to develop new, effective, safer, and more affordable anticancer drugs from alternative sources. In recent years, plant-derived Chinese medicine monomers have drawn increasing attention due to their high safety, low toxicity, minimal side effects, and antitumor effects. Plant-derived Chinese medicine monomers are effective against multiple targets and can regulate the growth, proliferation, apoptosis, invasion, and migration of OC as well as reverse drug resistance by regulating the Wnt/β-catenin signaling pathway. In this review, we summarize and provide mechanisms and prospects for the use of plant-derived Chinese medicines for the prevention and treatment of OC.

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“…Vimentin positive cells are only sporadically observed inside the tumorigenic tissue of our samples. This is in contrast to epithelial carcinomas in which high vimentin expression is correlated to the grade of malignancy and metastasis 31,32 . We hypothesize that the benign ameloblastoma tissue is in a state of intermediary EMT granting the tissue the capacity to produce collagenases for local invasion but without the ability to metastasize.…”

Section: Discussionmentioning

confidence: 62%

Differential expression of fibroblast activation protein-alpha and lysyl oxidase in subtypes of ameloblastoma

Vangansewinkel

Driesen

Agbaje

et al. 2023

Preprint

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Ameloblastomas are benign, mostly intra-osseous, tumours originating from ectodermal odontogenic epithelium and display extracellular matrix remodelling. We determined whether fibroblast activation protein-alpha (FAP-alpha), a collagenolytic enzyme and lysyl oxidase (LOX), a collagen cross-linker were differentially regulated in ameloblastoma subtypes. Masson trichrome staining and immunohistochemistry were performed on human samples from gross mandibular or maxillary ameloblastoma. Co-localization studies in tumorigenic tissue of follicular, plexiform and desmoplastic ameloblastoma showed absence of the mesenchymal cell marker vimentin but high epithelial cell adhesion molecule (EPCAM) expression, an epithelial marker. Strong FAP-alpha; staining was also noted, however, the stellate reticulum of plexiform ameloblastoma contained a higher number of FAP-alpha; positive cells than the follicular subtype. Weak LOX staining was demonstrated in tumorigenic tissue of the follicular subtype but profound reactivity was observed in stromal plexiform and tumorigenic desmoplastic tissue. The desmoplastic staining profile for FAP-alpha; and LOX shared resemblance with the unicystic subtypes. Tumorigenic outgrowths in desmoplastic ameloblastoma were associated with vimentin positive and EPCAM negative cancer-associated fibroblasts suggesting a role in tumour invasion. In conclusion, FAP-alpha; expression in ameloblastoma seems to be involved in tumour growth and invasion whereas LOX expression is differentially regulated in ameloblastoma subtypes offering a new perspective for understanding specific growth patterns of ameloblastoma subtypes.

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Epithelial–mesenchymal transition in serous and mucinous epithelial tumors of the ovary

Cited by 13 publications

References 2 publications

Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells

Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells

Plant-Derived Chinese Medicine Monomers on Ovarian Cancer via the Wnt/β-Catenin Signaling Pathway: Review of Mechanisms and Prospects

Differential expression of fibroblast activation protein-alpha and lysyl oxidase in subtypes of ameloblastoma

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