Epithelial differentiation patterns in buccal mucosa affected by lichen planus

Klein‐Szanto, Andres J.; Andersen, Lis Smedegaard; Schroeder, Hubert E.

doi:10.1007/bf02889219

Cited by 16 publications

References 22 publications

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A quantitative study of lamina densa alterations in hamster cheek pouch carcinogenesis

White

Gohari

1981

The Journal of Pathology

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Lesions produced by topical application of 0.5 per cent. DMBA to the hamster cheek pouch epithelium were classified as hyperplasia, dysplasia and carcinoma groups using strict histological criteria. Untreated epithelium served as a control. Tissue samples from five animals in each group were processed for electron microscopy and electron micrographs from the epithelial-connective tissue junction were obtained from 5 blocks per animal. The micrographs were subjected to stereological intersection counting to determine the relative surface (SSLD, BM) of lamina densa which was in normal relationship to the basal cell plasma membranes. Quantitative results indicated a progressive loss of lamina densa during carcinogenesis and this was accompanied by the extrusion of pseudopodia from the basal cells through the gaps. The pseudopodia were frequently related to peripheral cytoplasmic microfilaments. Quantitative data confirmed the progressive nature of this loss, with values for SSLD,BM being of the order of 98 per cent., 88 per cent., 76 per cent, and 42 per cent. for normal epithelium and for the hyperplastic, dysplastic and carcinomatous lesions respectively. The loss of lamina densa is discussed in relation to the specificity of the response and to the development of features indicative of motility in transforming cells.

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A quantitative study of lamina densa alterations in hamster cheek pouch carcinogenesis

White

Gohari

1981

The Journal of Pathology

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Towards economy of effort in quantitative ultrastructural pathology: Efficient sampling schemes for studying experimental carcinogenesis

Mayhew

White

Gohari

1982

The Journal of Pathology

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Hamster cheek pouch mucosa was painted with DMBA in order to compare samples of epithelium at different pathological stages of carcinogenesis. Stereological methods were applied to ultrathin sections of tissue to estimate one ultrastructural index of change, the surface ratio of lamina densa compared with the overlying plasma membrane of cells in stratum basale. Analysis of variance techniques were then employed to isolate and quantify the contributions which different levels of sampling (animals, tissue blocks, microscopic fields) made to the total observed variation in this surface ratio. Economical sampling schemes for future use were calculated from the sampling variances by taking into account the relative costs at each sampling level. Though illustrated by means of the hamster cheek pouch-DMBA model, our results are pertinent to many other experimental models for quantitative histopathology.

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The Application of Morphometric and Stereological Principles to Epithelial Tissues: Theoretical and Practical Considerations

White

1986

Basic and Applied Aspects of Noise-Induced Hearing Loss

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Epithelial differentiation patterns in buccal mucosa affected by lichen planus

Cited by 16 publications

References 22 publications

A quantitative study of lamina densa alterations in hamster cheek pouch carcinogenesis

A quantitative study of lamina densa alterations in hamster cheek pouch carcinogenesis

Towards economy of effort in quantitative ultrastructural pathology: Efficient sampling schemes for studying experimental carcinogenesis

The Application of Morphometric and Stereological Principles to Epithelial Tissues: Theoretical and Practical Considerations

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