The Application of Morphometric and Stereological Principles to Epithelial Tissues: Theoretical and Practical Considerations

White, F. H.

doi:10.1007/978-1-4684-5176-4_7

Basic and Applied Aspects of Noise-Induced Hearing Loss 1986

DOI: 10.1007/978-1-4684-5176-4_7

|View full text |Cite

The Application of Morphometric and Stereological Principles to Epithelial Tissues: Theoretical and Practical Considerations

F. H. White

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1994

1995

Publication Types

Select...

Article2

Relationship

Self Cite2

Independent0

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Morphometry and stereology are quantitative structural techniques which enable objective information to be acquired from sections of cells and tissues. These methods have been applied to studies of normal and abnormal tissues (5)(6)(7)(8)(9)(10)(11)(12)(13)(14), including lichen planus lesions (15)(16)(17)(18)(19)(20). and their objectivity may find application in differential diagnosis and prognosis (21)(22)(23).…”

mentioning

confidence: 99%

Quantitative cellular and nuclear volumetric alterations in epithelium from lichen planus lesions of human buccal mucosa

White

Jin

Yang

1994

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

Patients with oral lichen planus lesions may represent a relatively high risk population for subsequent development of oral cancer. Little is known of the relative effects of chronic inflammation and the process of malignant transformation itself on the histological structure of transforming epithelia. We have assessed cellular and nuclear volumes in defined basal and spinous cells from normal buccal mucosa epithelium, from epithelium associated with a non-specific chronic inflammatory infiltrate and from lichen planus lesions. Normal (N) tissues were obtained from the margins of non-neoplastic buccal mucosa lesions. Inflammatory (INF) lesions were from areas of the buccal mucosa diagnosed clinically as traumatic irritation without ulceration, and lichen planus (LI) lesions were biopsied from areas exhibiting Wickham's striae. Basal and spinous epithelial cells from normal and pathological human buccal mucosa were measured on haematoxylin and eosin-stained sections imaged through a video camera using a Zeiss VIDAS analyser and from these measurements, nuclear (VN) and cellular (VCELL) volumes were determined. VN and VCELL derived for both basal and spinous strata were similar in N and INF groups but were almost doubled in the LI group. Comparisons between LI and all other groups were significantly elevated. The effects of the inflammatory infiltrate on the oral epithelium in lichen planus and in non-specific inflammation thus differ significantly. VN and VCELL may serve as potential discriminators between benign lesions and premalignant lichen planus.

show abstract

mentioning

confidence: 99%

Quantitative cellular and nuclear volumetric alterations in epithelium from lichen planus lesions of human buccal mucosa

White

Jin

Yang

1994

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

Preliminary assessment of the epithelial nuclear‐cytoplasmic ratio and nuclear volume density in human palatal lesions

Jin

Yang

White

1995

J Oral Pathology Medicine

Self Cite

View full text Add to dashboard Cite

We have analysed both the nuclear-cytoplasmic (N/C) ratio and nuclear volume densities (VVN) in defined strata from human hard palate lesions with and without malignant potential to determine the prognostic reliability and/or validity of this parameter. Measurements of cellular and nuclear areas of basal and spinous cells from normal (N) and pathological palatal epithelium were made on histological sections using an image analyser. The lesions comprised fibrous hyperplasia (FH), traumatic inflammation (INF), benign hyperkeratosis (HK), squamous cell papilloma (PP), dysplastic epithelium adjacent to invasive carcinoma (CE) and islands of invasive squamous cell carcinoma (CI). In basal cells, no significant differences were detected in comparisons of N/C and VVN between all pathological groups and the N control group. The mean value for CE was lower than that obtained for N. In spinous cells, the only statistically significant comparison was between IF and FH for both N/C and VVN. Both parameters were lower in CE than in N. Of all groups analysed except CI, the CE group is the only one likely to possess an increased malignant potential. The N/C ratio therefore seems to be of no value as a predictor of malignancy in palatal epithelial lesions.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

The Application of Morphometric and Stereological Principles to Epithelial Tissues: Theoretical and Practical Considerations

Cited by 2 publications

References 83 publications

Quantitative cellular and nuclear volumetric alterations in epithelium from lichen planus lesions of human buccal mucosa

Quantitative cellular and nuclear volumetric alterations in epithelium from lichen planus lesions of human buccal mucosa

Preliminary assessment of the epithelial nuclear‐cytoplasmic ratio and nuclear volume density in human palatal lesions

Contact Info

Product

Resources

About