Epilobium Sp. (Willow Herb): Micropropagation and Production of Secondary Metabolites

Deliu, Constantin; Coste, Ana; Martos, Tamás

doi:10.1007/978-3-642-29974-2_6

Cited by 5 publications

(5 citation statements)

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“…angustifolium is extremely scarce and includes only one report published by Turker and co-workers in 2008 [18]. There are only a few papers on in vitro propagation of related species: E. parviflorum and E. hirsutum [19][20][21]. Turker's protocol describes rapid and efficient induction of shoots from seedling explants via direct organogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…To minimize browning of explants some antioxidants [23,24] or absorbents, like activated charcoal or polyvinylpyrrolidone (PVP), are added into media [25]. Some authors suggest pre-soaking of explants in antioxidant mixture to prevent oxidation of polyphenols [26,21]. By adding vitamin C (100 mg/l) into all prepared media Turker and his co-workers eliminated the problem.…”

Section: Discussionmentioning

confidence: 99%

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Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Dreger

Wegenke²,

Makowiecka

et al. 2016

Herba Polonica

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Summary Introduction: Willow herb (Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L. from Onagraceae family is a valuable medicinal plant that has been used in the treatment of urogenital disorders including BPH (Benign Prostatic Hypertrophy). The raw material is a rich source of polyphenols as well as steroids, triterpenoids and fatty acids. Due to frequent interspecific hybridization, plants collected in wild display a very diverse and variable content of active compounds. This poses a challenge in obtaining high quality and homogenous raw material. Application of the in vitro cultures and micropropagation techniques may offer a solution for alternative methods of cultivation. Objective: This work presents preliminary results of the first implementation of in vitro cultures of willow herb to obtain material for medicinal purposes. Methods: Sterile seedlings were donors of explants, which were used for induction of multi-shoots culture according to a modified protocol described by Turker and co-workers. Statistical analysis was used for assessment of significance of differences among variables. Results: Six different genotypes (lines) originating from root explants were chosen for clonal propagation. Efficiency of the elaborated method was 16–20 shoots per explant. Finally, over 3000 acclimatized plants were obtained and used for field crops. Conclusions: The use of Ch. angustifolium in vitro cultures can contribute to the introduction of this valuable herb for field crops and increase the availability of the raw material for food and pharmaceutical industries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Dreger

Wegenke²,

Makowiecka

et al. 2016

Herba Polonica

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“…In our study, the presence of β-sitosterol in E. hirsutum populations is reported for the first time. Considering the high yield of shoot biomass, a higher level of metabolites is expected in E. hirsutum compared with E. parviflorum ( Constantin et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the plant has been suggested for the treatment of skin diseases and inflammation such as eczema, acne, burns, and ulcers in traditional medicine ( Vogl et al, 2013 ). The plant has also been successfully applied for bladder health maintenance, male health maintenance, hormonal imbalances, and urinary system health ( Constantin et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analyses of Phytochemical Variation Within and Between Congeneric Species of Willow Herb, Epilobium hirsutum and E. parviflorum: Contribution of Environmental Factors

2021

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The plants in the Epilobium genus are considered to have several important medicinal properties due to their unique chemical composition. Although metabolic profiles of medicinal plants are mainly controlled by genetic factors, their production is also to some degree influenced by environmental factors, thus, variations in the levels of phytochemicals may represent long-term ecological and evolutionary interactions. In order to depict the magnitude of natural variation in level of chemical compounds among conspecific populations of Epilobium hirsutum (n = 31) and E. parviflorum (n = 16), metabolite profiling of aerial parts of plants was performed with gas chromatography/mass spectrometry analysis. Putative identification and structure annotation revealed the presence of 74 compounds including 46 compounds considered secondary metabolites categorized into flavonoids (n = 8), phenolic acids (n = 26), steroids (n = 3), and terpenes (n = 5) across all populations. Although there was a considerable natural variation among conspecific populations, principal component analysis revealed a clear separation of populations of each species based on the second main principal component which was highly correlated with eight secondary metabolites. The level of secondary metabolites was significantly correlated between species (r = 0.91), suggesting shared metabolic pathways underlying the production of chemical compounds. In addition, redundancy and variance partitioning analyses by including bioclimatic variables and altitude revealed a significant contribution of elevation in explaining the total variation of secondary metabolites in E. hirsutum. Two-thirds of all secondary metabolites were significantly correlated with altitude in E. hirsutum. The large-scale geographic analyses of populations revealed additionally detected flavonoids and terpenes (E. hirsutum and E. parviflorum) and steroids (E. hirsutum) for the first time. This study provides significant information on additional chemical compounds found across the distribution range of the two ecologically important species of willow herb and emphasizes the importance of geographic-wide sampling as a valuable strategy to depict intraspecific and interspecific variability in chemical traits.

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“…Крім того, на сьогодні ще недостатньо морфологічних даних, які б підтверджували таксономічний статус Chamaenerium, як таксона родового рангу. Рід Epilobium L. s. l. є одним з найчисельніших в родині Onagraceae Juss., який включає понад 200 видів [CONSTANTIN et al, 2013], поширених в основному в помірних, субарктичних та субантарктичних регіонах, рідше -в субтропіках та тропіках. У флорі України рід Epilobium s. l. за літературними даними [MOSYAKIN, FEDORONCHUK, 1999] представлений 21 видом.…”

unclassified

A brief overview of the taxonomy history of the genus Epilobium s.l.

Fedoronchuk¹

2019

Chornomors’k. bot. z.

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Epilobium Sp. (Willow Herb): Micropropagation and Production of Secondary Metabolites

Cited by 5 publications

References 79 publications

Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Comparative Analyses of Phytochemical Variation Within and Between Congeneric Species of Willow Herb, Epilobium hirsutum and E. parviflorum: Contribution of Environmental Factors

A brief overview of the taxonomy history of the genus Epilobium s.l.

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