Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Abstract

Summary Introduction: Willow herb (Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L. from Onagraceae family is a valuable medicinal plant that has been used in the treatment of urogenital disorders including BPH (Benign Prostatic Hypertrophy). The raw material is a rich source of polyphenols as well as steroids, triterpenoids and fatty acids. Due to frequent interspecific hybridization, plants collected in wild display a very diverse and variable content of active compounds. This poses a challenge in obtaining high quality and homogenous raw material. Application of the in vitro cultures and micropropagation techniques may offer a solution for alternative methods of cultivation. Objective: This work presents preliminary results of the first implementation of in vitro cultures of willow herb to obtain material for medicinal purposes. Methods: Sterile seedlings were donors of explants, which were used for induction of multi-shoots culture according to a modified protocol described by Turker and co-workers. Statistical analysis was used for assessment of significance of differences among variables. Results: Six different genotypes (lines) originating from root explants were chosen for clonal propagation. Efficiency of the elaborated method was 16–20 shoots per explant. Finally, over 3000 acclimatized plants were obtained and used for field crops. Conclusions: The use of Ch. angustifolium in vitro cultures can contribute to the introduction of this valuable herb for field crops and increase the availability of the raw material for food and pharmaceutical industries.

Micropropagation of Epilobium canum garretti (Firechalice) by Axillary Shoot Culture

“…Firechalice stem explants exuded a black substance, most likely a phenolic compound, but an antioxidant compound was unnecessary in the medium if leaves were kept above the medium and explant stems were cut without crushing. Leaf, stem, and root cultures of E. angustifolium (Dreger et al, 2016) and nodal cultures of E. parviflorum and E. hirsutum (Deliu et al, 2013) readily established on MS medium. Dreger et al (2016) used 0.1 g · L -1 ascorbic acid in the culture medium for stem segments and leaf explants of E. angustifolium because of browning and tissue necrosis of the explants.…”

“…Leaf, stem, and root cultures of E. angustifolium (Dreger et al, 2016) and nodal cultures of E. parviflorum and E. hirsutum (Deliu et al, 2013) readily established on MS medium. Dreger et al (2016) used 0.1 g · L -1 ascorbic acid in the culture medium for stem segments and leaf explants of E. angustifolium because of browning and tissue necrosis of the explants. Nodal explants of E. parviflorum and E. hirsutum also produced phenolic compounds in culture, but the intensity was low enough to avoid the use of antioxidant compounds in the media (Deliu et al, 2013).…”

“…Three Epilobium species-Epilobium parviflorum (Deliu et al, 2007), Epilobium hirsutum (T amas x et al, 2009), and Epilobium angustifolium (syn. Chamaenerion angustifolium) (Deliu et al, 2013;Dreger et al, 2016) have been micropropagated mainly by adventitious shoot regeneration from seedling tissues. Dreger et al (2016) used shoot tips to reproduce E. angustifolium but found that they were less successful for producing new shoots compared with using adventitious shoot regeneration.…”

Prevention of Phenolic Exudation of Chamaenerion angustifolium (L.) Scop. in in vitro culture

“…Наиболее интересной группой являются макроциклические эллаготанины (энотеины B и A и их производные). Основным активным компонентом растительного материала является энотеин В (2-14%) [2]. Практическое осуществление семенного способа размножения связано с рядом трудностей, обусловленных биологическими особенностями процесса прорастания семян и развития всходов [3,4].…”

“…В качестве заключения к изложенному материалу следует отметить, что яйца в кладке сизой чайки различаются не только по морфологическим показателям [2], но и активностью биохимических процессов в инкубируемых яйцах в зависимости от очередности их откладки. Исследование яиц из возобновленных кладок показали вариабельность значение биохимических индикаторов в зависимости от ранга яиц с более высокими значениями в первых отложенных яйцах.…”