Epidermal stem cells in wound healing and their clinical applications

Yang, Ronghua; Liu, Fengxia; Wang, Jingru; Chen, Xiao; Xie, Julin; Xiong, Kun

doi:10.1186/s13287-019-1312-z

Cited by 129 publications

(105 citation statements)

References 146 publications

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“…Epidermal SCs are a critical factor in skin homeostasis and wound healing. Distinct subtypes of epidermal SCs reside in the following areas: in the interfollicular epidermis (iSCs), in the hair follicles (hair follicle SCs, hSCs), and in the sebaceous glands (sebaceous gland SCs, sSCs) or sweat glands [6]. In postnatal skin, the interfollicular epidermal cells continuously proliferate and differentiate; thus, requiring continuous action of iSCs to maintain homeostasis.…”

Section: The Origin Differentiation and Regulation Of Epidermal Stementioning

confidence: 99%

“…Meanwhile, iSCs express low levels of transferrin receptor (CD71) and desmoglein-3. iSCs can also be traced in K14-CreER or Inv-CreER mouse strains [6,28]. Additional lineage tracing with Dlx1-CreER and Slc1a3-CreER reporters has identified two SC populations [29].…”

Section: The Origin Differentiation and Regulation Of Epidermal Stementioning

confidence: 99%

“…Finally, restructuring of the extracellular matrix occurs during the remodeling phase, and it may lead to scar formation [5]. Stem cells (SCs) are characterized by their potential for self-renewal and differentiation into other cell types [6]. Cutaneous SCs play an essential role in wound healing, mostly based on their ability to repair cellular substrates and to enhance the migration of fibroblasts and keratinocytes, angiogenesis, and collagen and elastin production [7].…”

Section: Introductionmentioning

confidence: 99%

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Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization

et al. 2020

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The skin, which serves as the first barrier of the human body, is particularly susceptible to exogenous injuries. Skin wounds, including acute burns and chronic non-healing ulcers, are commonly observed in clinics. Healing of skin wounds is a complex process, consisting of infiltration of inflammatory cells, cellular proliferation, and tissue remodeling phases, which restore the integrity and functions of the skin. Epithelialization is involved in wound healing through re-establishing an intact keratinocyte layer. Epidermal stem cells are indispensable for epithelialization, and they are regulated by multiple proinflammatory cytokines or growth factors. In this review, we summarize recent advances in the effect of these cytokines on migration, proliferation, and differentiation processes of epidermal stem cells. We also introduce promising therapeutic strategies targeting epidermal stem cells or related proinflammatory cytokines for patients with skin wounds.

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Section: The Origin Differentiation and Regulation Of Epidermal Stementioning

confidence: 99%

Section: The Origin Differentiation and Regulation Of Epidermal Stementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization

et al. 2020

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“…Stem cell therapy is considered as a powerful tool for various clinical applications. Human stem cells can be obtained from different tissues in adults during their lifetime, including bone-marrow mesenchymal stem cells (BMSCs) [1], umbilical cord stem cells (UCSCs), adipose-derived stem cells (ADSCs), skeletal stem cells, and other types, such as dental pulp, skin, and placenta [2][3][4][5][6][7]. Some studies have defined the stem cells from bone marrow as standard mesenchymal stem cells (MSCs).…”

Section: Introductionmentioning

confidence: 99%

An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Chu

Phuong

Tien

et al. 2020

IJMS

123

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Bone marrow mesenchymal stem/stromal cells (BMSCs), which are known as multipotent cells, are widely used in the treatment of various diseases via their self-renewable, differentiation, and immunomodulatory properties. In-vitro and in-vivo studies have supported the understanding mechanisms, safety, and efficacy of BMSCs therapy in clinical applications. The number of clinical trials in phase I/II is accelerating; however, they are limited in the size of subjects, regulations, and standards for the preparation and transportation and administration of BMSCs, leading to inconsistency in the input and outcome of the therapy. Based on the International Society for Cellular Therapy guidelines, the characterization, isolation, cultivation, differentiation, and applications can be optimized and standardized, which are compliant with good manufacturing practice requirements to produce clinical-grade preparation of BMSCs. This review highlights and updates on the progress of production, as well as provides further challenges in the studies of BMSCs, for the approval of BMSCs widely in clinical application.

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“…Stem cells often show high proliferation potential, which is conducive to the rapid repair of tissue damage. 45,[47][48][49] We used CCK8 assay to…”

Section: Proliferative Ability Of P75++ Cjecs During Continuous Celmentioning

confidence: 99%

Conjunctival reconstruction via enrichment of human conjunctival epithelial stem cells by p75 through the NGF-p75-SALL2 signaling axis

Yan

Chen

et al. 2020

Stem Cells Translational Medicine

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Severe conjunctival diseases can cause significant conjunctival scarring, which seriously limits eye movement and affects patients' vision. Conjunctival reconstruction remains challenging due to the lack of efficient methods for stem cells enrichment. This study indicated that p75 positive conjunctival epithelial cells (CjECs) were mainly located in the basal layer of human conjunctival epithelium and showed an immature differentiation state in vivo. The p75 strongly positive (p75++) CjECs enriched by immuno-magnetic beads exhibited high expression of stem cell markers and low expression of differentiated keratins. During continuous cell passage cultivation, p75 ++ CjECs showed the strongest proliferation potential and were able to reconstruct the conjunctiva in vivo with the most complete structure and function. Exogenous addition of NGF promoted the differentiation of CjECs by increasing nuclear localization of SALL2 in p75++ CjECs while proNGF played an opposite role. Altogether, p75 ++ CjECs present stem cell characteristics and exhibit the strongest proliferation potential so can be used as seed cells for conjunctival reconstruction, and NGF-p75-SALL2 signaling pathway was involved in regulating the differentiation of CjECs.

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Epidermal stem cells in wound healing and their clinical applications

Cited by 129 publications

References 146 publications

Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization

Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization

An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Conjunctival reconstruction via enrichment of human conjunctival epithelial stem cells by p75 through the NGF-p75-SALL2 signaling axis

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