An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Chu, Dinh‐Toi; Phuong, Thuy Nguyen Thi; Tien, Nguyen Le Bao; Tran, Dang Khoa; Thanh, Vo Van; Quang, Thuy Luu; Truong, Dang Tien; Pham, Van Huy; Ngọc, Võ Trương Như; Chu-Dinh, Thien; Kushekhar, Kushi

doi:10.3390/ijms21030708

Cited by 125 publications

(90 citation statements)

References 193 publications

(216 reference statements)

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“…Currently, the role of BM-MSCs in colorectal cancer remains in dispute. 28 Human BM-MSCs were found to stimulate invasion, survival and tumorigenesis of colorectal cancer through the release of soluble neuregulin 1 (NRG1), activating the HER2/HER3-dependent PI3K/AKT signalling…”

Section: Discussionmentioning

confidence: 99%

“…25,26 Previous studies have demonstrated the success of BM-MSCs for the treatment of hematopoietic pathologies, musculoskeletal diseases, immune disorder diseases, neurodegenerative diseases, cardiovascular disorders, sporty injuries, and other diseases of the gastrointestinal and integumentary systems. 27 Under long-term expansion, BM-MSCs may undergo senescence and transformation that contribute to tumor development and metastasis, 28 and a recent study reported that BM-MSCs promoted lung cancer growth and metastasis. 29 However, the role of rat BM-MSCs in the biological behaviors of colorectal CSCs remains unclear until now.…”

Section: Introductionmentioning

confidence: 99%

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<p>Rat Bone Marrow-Derived Mesenchymal Stem Cells Promote the Migration and Invasion of Colorectal Cancer Stem Cells</p>

Zou¹,

Zhao²,

Li³

et al. 2020

OTT

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Background: Colorectal cancer is one of the most common cancers and the second leading cause of cancer-related deaths worldwide. Targeting cancer stem cells (CSCs) may be a novel strategy for the treatment of colorectal cancer. Previous studies have shown that bone marrowderived MSCs (BM-MSCs) promote tumor growth and metastasis. However, the role of rat BM-MSCs in the biological behaviors of colorectal CSCs remains unclear until now. Materials and Methods: BM-MSCs were isolated from rats and characterized. CSCs were enriched from HCT116 cells using the microsphere culture method, and the microspheres incubated for at least 10 passages were termed HCT116-CSCs that were characterized. The effects of rat BM-MSCs on migration and invasion of HCT116-CSCs were examined using transwell migration and invasion assays and xenograft tumor growth assay. Results: Rat BM-MSCs appeared typical stem cell morphology. Flow cytometry revealed positive CD29 and CD44 expression in rat BM-MSCs at passage 3, and rat BM-MSCs were found to differentiate into osteocytes following incubation in osteogenic induction medium. Microscopy, flow cytometric detection of stem cell surface markers, colony-formation assay and transwell migration and invasion assays characterized the successful preparation of HCT116-CSCs, and subcutaneous injection of HCT116-CSCs produced xenograft tumors in nude mice, while HE staining of the xenograft tumors displayed cancer specimen shapes. Transwell migration and invasion assays showed that rat BM-MSCs promoted the migration and invasion of HCT116-CSCs, and injection of rat BM-MSCs was found to promote the growth of the mouse xenograft tumor derived from HCT116-CSCs. Conclusion: Rat BM-MSCs promote the migration and invasion of colorectal CSCs, and colorectal CSCs may be a potential target for the therapy against colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Rat Bone Marrow-Derived Mesenchymal Stem Cells Promote the Migration and Invasion of Colorectal Cancer Stem Cells</p>

Zou¹,

Zhao²,

Li³

et al. 2020

OTT

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“…BMSCs have been proposed to be promising candidates for the treatment of various diseases, such as muscle, bone and cartilage diseases; blood-related diseases; immune system diseases; nervous system diseases; and injuries [23]. Recently, it was identi ed that BMSCs exert their therapeutic activity through a paracrine effect mediated by the release of small extracellular vesicles (EVs) [16].…”

Section: Discussionmentioning

confidence: 99%

Exosomes Derived from Cyclic Mechanical Stretch-exposed Bone Marrow Mesenchymal Stem Cells Inhibit RANKL-induced Osteoclastogenesis through the NF-κB Signaling Pathway

Xiao

Zuo

et al. 2020

Preprint

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Background: Skeletal unloading usually induces severe disuse osteoporosis (DOP), which often occurs in patients subjected to prolonged immobility or in spaceflight astronauts. Increasing evidence suggests that exosomes are important mediators in maintaining the balance between bone formation and bone resorption. We hypothesized that exosomes play a pivotal role in the maintenance of bone homeostasis through intercellular communication between bone marrow mesenchymal stem cells (BMSCs) and osteoclasts under mechanical loading.Methods: Cells were divided into cyclic mechanical stretch (CMS)-treated BMSCs and normal static-cultured BMSCs, and exosomes were extracted by ultracentrifugation. After incubation with CMS-treated BMSC-derived exosomes (CMS_Exos) or static-cultured BMSC-derived exosomes (static_Exos), the apoptosis rates of bone marrow macrophages (BMMs) were determined by flow cytometry, and cell viability was detected with a CCK-8 assay. Osteoclast differentiation was determined with an in vitro osteoclastogenesis assay. Signaling pathway activation was evaluated by Western blotting and immunofluorescence staining. Hindlimb unloading (HU)-induced DOP mouse models were prepared to evaluate the function of exosomes in DOP.Results: Both CMS_Exos and static_Exos could be internalized by BMMs, and CMS_Exos did not affect BMM viability or increase apoptosis. CMS_Exos effectively suppressed receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation and F-actin ring formation. Further molecular investigation demonstrated that CMS_Exos impaired osteoclast differentiation via inhibition of the RANKL-induced NF-κB signaling pathway. Both CMS_Exos and static_Exos partly rescued the osteoporosis caused by mechanical unloading; however, the CMS_Exo group showed more obvious rescue. CMS_Exo treatment significantly decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts.Conclusions: Exosomes derived from CMS-treated BMSCs strongly inhibited osteoclast differentiation by attenuating the NF-κB signaling pathway in vitro and rescued osteoporosis caused by mechanical unloading in an HU mouse model in vivo.

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“…Bone marrow (BM)-derived MSCs are non-HSCs located in the medullary stroma of bone marrow. BM-MSCs were discovered by Friedenstein et al in 1976 (25). Typically, they are extracted from iliac, femur, tibia, and sternum bone.…”

Section: Msc Tissue-derived Mscsmentioning

confidence: 99%

Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases

Chung

Son

Park

et al. 2021

IJSC

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An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Cited by 125 publications

References 193 publications

<p>Rat Bone Marrow-Derived Mesenchymal Stem Cells Promote the Migration and Invasion of Colorectal Cancer Stem Cells</p>

<p>Rat Bone Marrow-Derived Mesenchymal Stem Cells Promote the Migration and Invasion of Colorectal Cancer Stem Cells</p>

Exosomes Derived from Cyclic Mechanical Stretch-exposed Bone Marrow Mesenchymal Stem Cells Inhibit RANKL-induced Osteoclastogenesis through the NF-κB Signaling Pathway

Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases

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