Epidemiological investigation of Pseudomonas aeruginosa nosocomial bacteraemia isolates by PCR-based DNA fingerprinting analysis

Abstract

, 64 isolates of Pseudomonas aeruginosa were implicated in bacteraemia in 25 cancer patients in five wards of two hospitals. These, together with 24 environmental isolates and one isolate from a bacteraemia in a noncancer patient were examined by three PCR-based DNA fingerprinting methods: random amplified polymorphic DNA (RAPD), enterobacterial-repetitive intergenic consensus (ERIC)-PCR, and 16s-23s spacer region-based RAPD. These methods were reproducible, discriminatory and showed close agreement; all indicated that 47 isolates that had caused bacteraemia in 19 cancer patients were indistinguishable. Seventeen other isolates that had caused bacteraemia in 10 cancer patients were discriminated into eight further groups, and the 24 environmental and non-cancer patient isolates into further distinct groups. No environmental source of the epidemic strain was found, but it was suspected that the outbreak was related to infusion implants.

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)

“…Pyocin production was tested on selective Cetrimide Agar aeruginosa strains isolated from faeces (10,11,12,13,14), NCTC 6749 (15) and wound (16,17,18 Results of antibiotic resistance and genotyping showed poor correlation. Resistance patterns from bacterial isolates which had identical genotypes differed in up to 9 antibiotics.…”

“…DNA typing methods have been frequently used to investigate the diversity of collections of P. aeruginosa (20). These methods include pulsed-field gel electrophoresis (PFGE) (8, 21,22), ribotyping (6,8), restriction fragment length polymorphic DNA analysis (RFLP) (6), random amplified polymorphic DNA assay (RAPD) (8, 13,21), arbitrary primed PCR (AP-PCR) (4), amplified fragment length polymorphism (AFLP) (21), and repetitive element based PCR (rep-PCR) (6,22 The aim of this work was to estimate intra-species differentiation of P. aeruginosa strains isolated from two hospitals in Siedlce (Poland) using phenotypic methods (serotyping, susceptibility to chemotherapeutic agents, and type of growth on cetrimide agar medium) and the genotypic method (BOX-PCR).…”

BOX-PCR is an adequate tool for typing of clinical <i>Pseudomonas aeruginosa</i> isolates

“…Consequently, the microorganism can be found frequently in the hospital environment and home reservoirs such as sinks, floors, baths, soapdishes and dishcloths. Pseudomonas aeruginosa is considered an opportunistic pathogen as it causes hospital-acquired infection, particularly in immunocompromised hosts, burn victims or in those with faulty homeostasis mechanisms or metabolic disorders [1][2][3].…”

“…DNA typing methods have been frequently used for investigating the diversity of collections of Pseudomonas aeruginosa [4]. These methods include pulsed-field gel electrophoresis (PFGE) [5,6], ribotyping [7], restriction fragment length polymorphic DNA analysis (RFLP) [7], random amplified polymorphic DNA assay (RAPD) [3,5], arbitrary primed PCR (AP-PCR) [3], amplified fragment length polymorphism (AFLP) [5], and repetitive element based PCR (rep-PCR) [3,6,7].…”

Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil

“…10 Investigations of the nosocomial epidemiology of P. aeruginosa have been hampered by the inadequate discriminatory ability of classical phenotypic methods such as serotyping, phage and pyocin typing, and biotyping. 11,12 Modern DNA-based techniques, such as enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and Ribotyping, have been widely used in the epidemiological investigation of many microorganisms, including P. aeruginosa. 13 In the present study, genotypic and phenotypic characteristics of 56 Pseudomonas aeruginosa isolates obtained from 13 cystic fibrosis patients have been evaluated.…”