Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)

Wolska, Katarzyna; Kot, Barbara; Jakubczak, A.

doi:10.1590/s1517-83822012000100032

Cited by 23 publications

(21 citation statements)

References 11 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among all the investigating tools, DNA typing techniques have been often used to investigate the divergence of P. aeruginosa (14). Proteins can also be identify by matrix-assisted leser desorption/ionization time-of-ight mass spectrometry (MALDI-TOF MS) (15).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Chand¹,

Khanal²,

Panta³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa is an opportunistic human pathogen and are reported to cause acute and chronic infectious diseases. Due to its high ability to acquire resistance to many antibiotics, it has become a global public health threat. It consists of some virulence genes that may lead to its pathogenicity. The main objective of this cross-sectional study was to detect the virulence genes and antibiotic susceptibility pattern of P. aeruginosa isolated from clinical specimens collected from governmental hospital of Nepal.Methods: A total of 7898 clinical specimens were analyzed for the period of six months from November 2018 to April 2019. The specimens were cultured on Nutrient agar, Blood agar, MacConkey agar, Chocolate agar, Cysteine-Lactose, Electrolyte Deficient agar plates and were incubated at 37°C for 24 hours. All the isolates were identified by standard biochemical tests and further confirmed by growth on Cetrimide agar plate. The antibiotic susceptibility testing was performed by modified Kirby-Bauer disc diffusion method following CLSI guideline. Multiplex-PCR was done to detect the virulence genes oprL and toxA. Statistical analysis was carried out using IBM SPSS Statistic ver. 25 and the p-value was calculated at significance level (0.05%) by using Chi square.Results: Out of these specimens investigated, 87 isolates were tentatively identified to be P. aeruginosa in which 20 (22.98 %) were found to be multidrug resistant. Comparatively, most of the P. aeruginosa were isolated from outpatients 63 (72.41 %) than inpatients 24 (27.58 %), from male 56 (64.36 %) than female 31 (35.63 %) and in age group 60-79 years (41.37 %). AST result showed the highest resistance of 100% with cefixime whereas susceptibilities of 83.9% and 81.6% with polymixin B and tobramycin were noticed respectively. The PCR results showed that all P. aeruginosa isolates carried oprL 87 (100%) and 83 (95.4 %) isolates showed toxA genes. Conclusion: The studies revealed that almost all P. aeruginosa harbors both oprL and toxA genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Chand¹,

Khanal²,

Panta³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Molecular typing methods have been used to study the genetic diversity of P. aeruginosa. DNA typing methods include ribotyping, pulsed field gel electrophoresis (PFGE), repetitive element based PCR (rep-PCR) and random amplification of polymorphic DNA (RAPD) [13] [14]. RAPD-PCR is one of the molecular techniques used for P. aeruginosa typing.…”

Section: Introductionmentioning

confidence: 99%

Serotypes, Antibiogram and Genetic Relatedness of <i>Pseudomonas aeruginosa</i> Isolates from Urinary Tract Infections at Urology and Nephrology Center, Mansoura, Egypt

Abdel-Rhman¹,

Rizk²

2018

AiM

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that represents a major problem in many hospitals because of its increased resistance to antibiotics and the ability to cause nosocomial infections. The present study aimed to phenotype and genotype isolates of P. aeruginosa from inpatients with UTIs at Urology and Nephrology center, Mansoura, Egypt to study their relatedness. Methods: Thirty nine isolates of P. aeruginosa were phenotypically typed by determination of O-serotypes by slide agglutination technique and antimicrobial resistance patterns by disk-diffusion method. The genetic diversity of isolates was illustrated by performing RAPD-PCR using M13 primer. Results: Serotypes O11, O6 and O10 were the most prevalent. Isolates showed high resistance rates to antipseudmonal antibiotics with high incidence (51.3%) of multidrug resistance (MDR). Amikacin was the most effective. A significant correlation was found between O6, O10 and MDR. A relatively high polymorphism was demonstrated among P. aeruginosa isolates by using RAPD-M13 fingerprinting. Cross transmission was suggested by phenotypically and clonally identical isolates. Conclusion: The study demonstrates the role of combining both classical and molecular typing as a valuable mean to study the origin and cross transmission of P. aeruginosa in UTIs for better assessment of treatment and infection control.

show abstract

“…In order to find an epidemiological link between the strains of P. aeruginosa isolated in hospital, phenotypic markers such as antibiotype and serotype are used to differentiate strains before genetic characterization (Blot et al, 2013;Wolska et al, 2012). There is paucity of data on P.aeruginosa infections in Côted' Ivoire.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic susceptibility and serotype patterns of Pseudomonas aeruginosa from clinical isolates in Abidjan, Cote dIvoire

Elogne¹,

Kalpy²,

Yéo³

et al. 2018

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Pseudomonas aeruginosa (P. aeruginosa) has been found to be a common hospital acquired pathogen, responsible for several severe infections. The objective of this study was to describe the antibiotic resistance profile of P. aeruginosa strains from human sample. This descriptive study was carried out on 168 isolated strains of P. aeruginosa collected from January 2014 to December 2015 at the Pasteur Institute of Côte d'Ivoire. The strains identification was done according to the methods of conventional bacteriology. The antibiotic sensitivity tests were performed using the disc diffusion method in agar medium according to CA-SFM (Antibiogram committee of French society of microbiology) criteria. The serotyping of the strains was carried out by using the agglutination method slide test, with the aid of 4 specific polyvalent antisera. The most prevalent P. aeruginosa serotypes were O4 (24.4); O11 (14.6); and O6 (9.5%). The rate of antibiotic resistance to ticarcillin was 32.9%, ciprofloxacin 18.4%, ceftazidime 14.9%, Imipenem 11.3%, and amikacin 11.3%. Resistance to Imipenem was above 10% in an intensive care unit and in the pneumonology unit (PPH). Strains of O6 serotypes were the most multidrugresistant followed by O11 with respective rates of 31.2 and 28% MDR (Multidrug Resistance). P. aeruginsa are microorganism capable of developing mechanisms of complex resistance which makes it difficult to manage. The attention of hygiene rules and the rational use of antibiotics are very important in other to prevent the spread of MDR P. aeruginosa.

show abstract

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)

Cited by 23 publications

References 11 publications

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Serotypes, Antibiogram and Genetic Relatedness of <i>Pseudomonas aeruginosa</i> Isolates from Urinary Tract Infections at Urology and Nephrology Center, Mansoura, Egypt

Antibiotic susceptibility and serotype patterns of Pseudomonas aeruginosa from clinical isolates in Abidjan, Cote dIvoire

Contact Info

Product

Resources

About

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)

Cited by 23 publications

References 11 publications

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Serotypes, Antibiogram and Genetic Relatedness of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Isolates from Urinary Tract Infections at Urology and Nephrology Center, Mansoura, Egypt

Antibiotic susceptibility and serotype patterns of Pseudomonas aeruginosa from clinical isolates in Abidjan, Cote dIvoire

Contact Info

Product

Resources

About

Serotypes, Antibiogram and Genetic Relatedness of <i>Pseudomonas aeruginosa</i> Isolates from Urinary Tract Infections at Urology and Nephrology Center, Mansoura, Egypt