Epidemiological and Phylogenetic Analysis of Spanish HumanBrucella melitensisStrains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, andrpoBTyping

Abstract

The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase ␤ subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (

Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16

“…It is important to note that the major genotypes have shown low allelic polymorphisms at the MLVA-16 loci. Similar findings were reported in the aforementioned countries (Al Dahouk et al, 2007;Ferreira et al, 2012;Jiang et al, 2011Jiang et al, , 2013Kilic et al, 2011;Valdezate et al, 2010). Only two strains belonged to genotype 43, which is mainly found in the Mediterranean region (Greece, Spain, Lebanon, Syria, Turkey, France, and Italy).…”

“…Only two strains belonged to genotype 43, which is mainly found in the Mediterranean region (Greece, Spain, Lebanon, Syria, Turkey, France, and Italy). In addition, genotype 43 was reported in Germany and China and is widespread in Middle Eastern countries (Lebanon and Turkey) (Aftab et al, 2011;Al Dahouk et al, 2007; genotype 63 is less common, represented by only 19 strains, 12 of which were from China with the remainder from Turkey, Spain, and France (Aftab et al, 2011;Al Dahouk et al, 2007;Jiang et al, 2011Jiang et al, , 2013Kilic et al, 2011;Valdezate et al, 2010). In the current study 19 strains of B. melitensis were identified as genotype 63.…”

MLVA Genotyping of Brucella melitensis and Brucella abortus Isolates from Different Animal Species and Humans and Identification of Brucella suis Vaccine Strain S2 from Cattle in China

“…Whole genome sequencing of isolates from this lineage might provide information about host adaptation and microevolution. MLVA8 genotype 42, ‘East Mediterranean’ group (humans and animals isolates), as we have shown, is widely distributed throughout China, and has previously been reported to be predominant in Turkey, Portugal and Spain [19,24,27,28]. In Zhejiang province, an area with low prevalence of brucellosis, applying MLVA added valuable information to the epidemiological investigation and could be used as a tool to follow the spread and control of the disease.…”

Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century

“…The ability of VNTR analysis to link genotypes to background epidemiological data should also facilitate the identification of risk factors and help understand differential virulence or pathogenic properties of individual genotypes. VNTR has recently proven highly efficient in confirming laboratory or other professionally acquired infections (Marianelli et al, 2008;Valdezate et al, 2010), in distinguishing relapse from re-infection (Al Dahouk et al, 2005a;Kattar et al, 2008) in characterizing outbreaks (Lucero et al, 2010;Valdezate et al, 2007), in identifying associations of different genotypes with different pathogenic profiles (Nockler et al, 2009), and in assessing the stability of vaccine preparations (Garcia-Yoldi et al, 2007).…”