Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century

Godfroid, Jacques; Scholz, Holger C.; Barbier, Thibault; Nicolas, Cécile; Wattiau, Pierre; Frétin, David; Whatmore, Adrian M.; Cloeckaert, Axel; Blasco, J.M.; Moriyón, Ignacio; Saegerman, Claude; Muma, John Bwalya; Dahouk, Sascha Al; Neubauer, Heinrich; Letesson, Jean‐Jacques

doi:10.1016/j.prevetmed.2011.04.007

Cited by 335 publications

(334 citation statements)

References 138 publications

(146 reference statements)

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“…In other studies, the consumption of raw milk has been shown to be the most significant risk factor for the transmission of brucellosis among humans (Godfroid et al, 2011). However, in this study, consumption of unpasteurized dairy products was not a significant risk factor.…”

Section: Rahman Et Alcontrasting

confidence: 72%

Seroprevalence and Risk Factors for Brucellosis in a High-Risk Group of Individuals in Bangladesh

Rahman

Berkvens

Frétin

et al. 2012

Foodborne Pathogens and Disease

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Brucellosis is an occupational hazard of livestock farmers, dairy workers, veterinarians, slaughterhouse workers, and laboratory personnel, all of whom are considered to belong to the high-risk occupational group (HROG). A study was undertaken to determine the seroprevalence of brucellosis, identify risk factors associated with brucellosis seropositivity, and detect Brucella at genus level using real-time polymerase chain reaction (PCR) among people in the HROG in the Dhaka division of Bangladesh. A sample of 500 individuals from the HROG was collected from three districts of Dhaka division of Bangladesh. A multiple random effects logistic regression model was used to identify potential risk factors. Two types of real-time PCR methods were applied to detect Brucella genus-specific DNA using serum from seropositive patients. The prevalence of brucellosis based on the three tests was observed to be 4.4% based on a parallel interpretation. The results of the multiple random effects logistic regression analysis with random intercept for district revealed that the odds of brucellosis seropositivity among individuals who had been in contact with livestock for more than 26 years was about 14 times higher as compared to those who had less than 5 years of contact with livestock. In addition, when the contact was with goats, the odds of brucellosis seropositivity were about 60 times higher as compared to when contact was with cattle only. Noticeable variation in brucellosis seropositivity among humans within the three districts was noted. All of the 13 individuals who tested positive for the serological tests were also positive in two types of real-time PCR using the same serum samples. Livestock farmers of brucellosis positive herds had a significantly higher probability to be seropositive for brucellosis. The study emphasized that contact with livestock, especially goats, is a significant risk factor for the transmission of brucellosis among individuals in the HROG.

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Section: Rahman Et Alcontrasting

confidence: 72%

Seroprevalence and Risk Factors for Brucellosis in a High-Risk Group of Individuals in Bangladesh

Rahman

Berkvens

Frétin

et al. 2012

Foodborne Pathogens and Disease

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“…It has been documented elsewhere that iELISA's have superior sensitivity to the RBT and CFT [12,33]. As a consequence, in known infected herds, animals classified positive by iELISA and negative by RBT and/or CFT, should be considered as infected [11].…”

Section: Evaluating the Brucellin Skin Test As An Additional Test Tomentioning

confidence: 99%

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

Nyanhongo¹

2017

SOJMID

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Brucellosis is a bacterial disease of high veterinary importance, besides being a significant zoonosis worldwide. It is characterised by variable, and at times long and latent, incubation periods, which makes its control and eradication a challenging effort. No single test is able to identify all stages of the disease with high sensitivity. The brucellin skin test, which has been proved to identify some acute and chronic latent stages of brucellosis, has not been validated in South Africa. The objective of this study was to evaluate the brucellin skin test in brucellosis free, as well as confirmed infected cattle herds, under South African commercial farming conditions. The results indicate that the brucellin skin test has a high specificity (99.2% (95% confidence interval (CI): 98.1% -99.7%)), and that despite a relatively low sensitivity (42.9 % (95% CI: 30.5% -56.0%)), it was able to identify all the infected herds and detect more infected heifers in infected herds when compared to the routinely used Rose Bengal Test and Complement Fixation Test. It was concluded that the brucellin skin test could be used in parallel with serological tests to improve the sensitivity of the current diagnostic strategy in chronically infected herds where eradication of the disease has proven problematic. Removal of more infected heifers would decrease infection pressure and streamline vaccination towards heifers not exposed to Brucella abortus.

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“…Currently the Brucella genus consists of 12 species with validly published names, based on host preferences, phenotypic differences and pathogenesis (Godfroid et al 2011, Whatmore et al 2014, Scholz et al 2016. While a broad host range generally exists for Brucella species, Brucella infection follows a very strict, host-related hierarchy of pathogenicity (Adam 2002).…”

Section: Introductionmentioning

confidence: 99%

The mismatched isolation of Brucella strains from nomic hosts

Büyük¹,

Sidamonidze²,

Zhgenti³

et al. 2017

EurasianJVetSci

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ÖzAmaç: Bu çalışmada Kafkas Üniversitesine ait Brucella suş koleksiyonunun bir bölümü çeşitli yöntemler ile değerlendi-rilmiştir. Öneri: Bu çalışma belli konaklar arasında çapraz Brucella infeksiyonunun sıradışı olarak nitelendirilebileceğini fakat zamanla bunun olağan olabileceğini göstermektedir. Bu şe-kildeki çalışmalar, Brucella cinsinin patojenitesini, konak spesifitesini ve gelişimini daha iyi anlamaya yardımcı olacaktır. Gereç veAnahtar kelimeler: Brucella, saha suşlar, sğır, koyun, moleküler karakterizasyon Abstract Aim: A small part of field Brucella strain collection of Kafkas University was evaluated by different methods in this study. Materials and Methods:The strains were originated from cattle and sheep which had a history of abortion. Thirty field Brucella strains (16 were originated from sheep and 14 were from cattle) were randomly selected and included in the study. In addition of standardized phenotypical characterization, genus specific real-time PCR and species specific Bruce-Ladder PCR were conducted for molecular typing.Results: Out of 16 sheep and 14 cattle samples 15 and 13 were identified as B. melitensis and B. abortus, respectively. Interestingly one strain was characterized as B. abortus isolated from aborted sheep foetus and the other as B. melitensis from aborted cattle milk sample. Furthermore one strain from aborted sheep foetus was unable to be confirmed as Brucella spp. by genotypically (neither by real-time PCR nor Bruce-Ladder PCR) though all phenotypic features were similar to Brucella genus and kept under strict conditions for future characterization on suspicion of Ochrobactrum spp. Conclusion:These report alleges that Brucella crossinfection among certain hosts ceased to be extraordinary and gained an usual face over time. These kind of efforts would help in further understanding the evolution, host specificity and pathogenicity of the genus Brucella.

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Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century

Cited by 335 publications

References 138 publications

Seroprevalence and Risk Factors for Brucellosis in a High-Risk Group of Individuals in Bangladesh

Seroprevalence and Risk Factors for Brucellosis in a High-Risk Group of Individuals in Bangladesh

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

The mismatched isolation of Brucella strains from nomic hosts

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