Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16

Shevtsov, Alexandr; Ramanculov, Erlan; Shevtsova, E. F.; Kairzhanova, A.D.; Tarlykov, Pavel; Филипенко, М. Л.; Дымова, М. А.; Abisheva, Gulzada; Jailbekova, Aygul; Камалова, Динара; Chsherbakov, Andrei; Tulegenov, Samat; Akhmetova, Assel; Sytnik, Igor; Karibaev, Talgat; Mukanov, Kasim

doi:10.1016/j.meegid.2015.07.008

Cited by 28 publications

(30 citation statements)

References 33 publications

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“…The combined analysis of 16 VNTR markers revealed a high level of genetic diversity (HGDI, 0.871). Attributable to the broader geographical coverage and temporal sampling interval, this is almost twice the value that was obtained in an earlier study [27]. Comparison of MLVA-11 profiles with MLVAbank data revealed four new genotypes.…”

Section: Discussionsupporting

confidence: 52%

“…Multiplex PCR and capillary electrophoresis (CE) were carried out as described by Garofolo et al with minor modifications [27, 33]. The sixteen pairs of primers were separated into three panels.…”

Section: Methodsmentioning

confidence: 99%

“…The resulted data were processed with BioNumerics 7.5 software (Applied Maths,). MLVA-16 profiles of five additional strains were included from our previous study (“key”: a_1, a_18, a_82, a_112 and a_121) [27], All of them were isolated from cattle between 2007 and 2013 and found to have different genotypes. Cluster analysis was based on the categorical coefficient and unweighted pair group method using arithmetic averages (UPGMA).…”

Section: Methodsmentioning

confidence: 99%

“…abortus isolated from animals found in two regions of Kazakhstan. Specifically, the index of genetic diversity (HGDI) for MLVA-16 was 0.491 [27]. The discriminatory ability of this study was not high enough for epidemiological monitoring, possibly due to the limited sampling area.…”

Section: Introductionmentioning

confidence: 94%

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Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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Brucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan. We analyzed official data on the incidence of animal brucellosis in Kazakhstan. The records span more than 70 years of anti-brucellosis campaigns, and contain a brief description of the applied control strategies, their effectiveness, and their impact on the incidence in humans. The MLVA-16 method was used to type 94 strains of B. abortus and serial passages of B. abortus 82, a strain used in vaccines. MLVA-8 and MLVA-11 analyses clustered strains into a total of four and seven genotypes, respectively; it is the first time that four of these genotypes have been described. MLVA-16 analysis divided strains into 28 distinct genotypes having genetic similarity coefficient that varies from 60 to100% and a Hunter & Gaston diversity index of 0.871. MST analysis reconstruction revealed clustering into "Kazakhstani-Chinese (Central Asian)", "European" and "American" lines. Detection of multiple genotypes in a single outbreak confirms that poorly controlled trade of livestock plays a crucial role in the spread of infection. Notably, the MLVA-16 profile of the B. abortus 82 strain was unique and did not change during 33 serial passages. MLVA genotyping may thus be useful for epidemiological monitoring of brucellosis, and for tracking the source(s) of infection. We suggest that countrywide application of MLVA genotyping would improve the control of brucellosis in Kazakhstan.

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Section: Discussionsupporting

confidence: 52%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

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Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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“…MLVA has been shown to be useful in revealing the most prevalent genotypes, distribution, circulation and relatedness of Brucella isolates, mainly in endemic areas [28][29][30].…”

Section: The Advanced Diagnostic Testsmentioning

confidence: 99%

New Scenarios for Brucella suis and Brucella melitensis

Samartino

Arregui

Martino³

2017

Curr Clin Micro Rpt

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Purpose of Review We pretend to highlight the most important advances reached in the last few years in the biology of Brucella suis and Brucella melitensis and focus attention on new tools for the comprehension, prevention and control of these zoonotic agents. Recent Findings Important progress lately done in the field of pathogenesis is presented here. This involves current studies on proteins involved in the survival and antigenic structure of Brucella, as well as the findings of how Brucella has achieved modifications in order to adapt, replicate, survive and modulate the host´s immune system, improve the knowledge of the performance of these bacteria. Summary Consequently, better approaches for vaccinology and diagnostic techniques are developed. Relevant epidemiological issues based on changes in the highly conserved genome of these bacteria will be valuable tools to describe outbreaks and disease status, along with risk factors that would otherwise be difficult to analyze.

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Genetic variation of Brucella isolates at strain level in Egypt

Abdel‐Hamid

Elbauomy

Ghobashy

et al. 2020

Veterinary Medicine & Sci

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In this study, Multiple Locus Variable Number Tandem Repeat Analysis (MLVA‐16) was performed on 18 Brucella isolates identified bacteriologically and molecularly (AMOS‐PCR) as Brucella abortus ( n = 6) and Brucella melitensis ( n = 12). This was aimed to study the genetic association among some Egyptian Brucella genotypes isolated during the period from 2002 to 2013 along with the global genotypes database. MLVA‐16 analysis for B. melitensis and B. abortus strains illustrates a total of 11, and 3 genotypes with 10 and 1 singleton genotypes, respectively. B. melitensis strains displayed greater markers diversity by VNTRs analysis of the 16 loci than B. abortus and this was attributed mainly to the diverging in panel 2B markers. B. melitensis genotype M4_Fayoum_Giza (3,5,3,13,1,1,3,3,8,21,8,7,5,9,5,3) was the only predominated genotype circulating between two different governorates. The most common B. abortus genotype, GT A3_Dakahlia (4,5,4,12,2,2,3,3,6,21,8,4,4,3,4,4), was present in three identical isolates. In phylogeny, Egyptian B. abortus bv1 genotypes were closely related to East Asian strain (for the first time), Western Mediterranean and Americas clonal lineages. B. melitensis local genotypes exhibit a genetic relatedness mostly to Western Mediterranean clonal lineage and one strain of Eastern Mediterranean clonal lineage. In conclusion, the geographic location is not the only factor stands behind the high genetic similarity of the Egyptian Brucella genotypes. These low variations may be a result of a stepwise mutational event of the most variable loci from a very limited number of ancestors especially during the transmission through non‐preference hosts. The authors encourage the authorities in charge to establish pre‐movement testing to reduce the risk of brucellosis spread.

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Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16

Cited by 28 publications

References 33 publications

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

New Scenarios for Brucella suis and Brucella melitensis

Genetic variation of Brucella isolates at strain level in Egypt

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