Eosinophilia as a Predictor Morbidity of Soil- Transmitted Helminthiases Among Widodaren Plantation Workers in Jember

Abstract

Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tropical and sub-tropical countries, including Indonesia. According to WHO, approximately 1,5 billion people or 24% of the world’s population, are infected with Soil-Transmitted Helminth (STH). Morbidity is directly related to the intensity of STH infestation. Some of the studies shown a significant correlation between eosinophilia and helminthiasis. Eosinophilia on the blood smear examination may be used as a marker of STH infestation. Purpose: to determine the correlation between Soil-Transmitted Helminthiases and eosinophilia as a predictor of morbidity of STH infestation among Widodaren Plantation Workers in Jember. Methods: This observational analytical study use the Kato-Katz method to determine infestation of STH and its intensity measured by egg count per gram stool (EPG). Blood smear examination stained with Giemsa conduct to determine eosinophil count. Results: Prevalence of STH infestation was 22,7% (15/66). Ascaris lumbricoides infection was 13,6% (9/66) and Hookworm infection was 4,5% (3/66). There is mixed infection between A. lumbricoides and Hookworm, the prevalence was 4,5%% (3/66). All of the infection intensity was light. The prevalence of eosinophilia was 27,2% (18/66). There was a significant correlation between STH infection and eosinophilia (P=0,000) but, there was a very weak correlation between the intensity of STH infection and eosinophilia (r=0,281; p=0,258). Conclusion: The prevalence of STH infection among Widodaren Plantation Workers is 22,7% and predominated by A. lumbriocides. There is a positive correlation between intensity of STH infection and eosinophilia as predictor morbidity of STH infection with very weak power.

Prevalence of Soil Transmitted Helminths in Elementary School Students with Behavioral Risk Factors

“…However, elementary school students who defecate in the yard or garden do not accumulate their feces, even though they sometimes still play in the garden or fields, so there are still cases of worm infection with A. lumbricoides and T. trichiura. According to Primadana (2019) and Riswanda (2016) clinical symptoms due to STH infestation in the gastrointestinal tract generally only appear when the intensity of the infestation is in the moderate and severe categories. The morbidity of the prevalence of worms is directly related to the prevalence of worms in the human body.…”

Increased of IL-4 and Ig E Level in Infection Hookworm in Plantation Workers in Jember

“…The result of this study can support the diagnosis of hookworm infection. The increase of IL-4 and IgE level could be as predictor of morbidity among the plantation worker, especially for hookworm infection as well as eosinophilia (Primadana et al, 2019). However, some mechanisms of immune respons in hookworm are still unclear and this could be a challenge.…”

PEMERIKSAAN MIKROSKOPIS KUALITAS SEDIAAN TELUR CACING Trichuris trichiura MENGGUNAKAN METODE NATIF DAN METODE FLOTASI

“…Kekurangan pemeriksaan flotasi pengerjaannnya cukup lama dan lebih cocok dipakai pada feses yang mengamdung sedikit telur cacing. Metode ini digunakan sebagai alternatif dikarenakan pada metode natif ini masih mempunyai kekurangan khususnya pada infeksi ringan [8] Menurut Sihombing (2019) [9] Tahapan pemeriksaan metode natif dan metode flotasi sebagai berikut: Pada pemeriksaan menggunakan metode natif pertama menyiapkan alat dan bahan yang akan digunakan, teteskan larutan Eosin 2% sebanyak 1-2 tetes diatas kaca objek, ambil feses dengan lidi (± 2 mg) letakan di atas kaca objek yang telah ditetesi eosin 2%, homogenkan feses diatas kaca objek dengan membentuk lingkaran, Tutup dengan deck glass ukuran 20 x 20 mm sampai kaca penutup rata menutupi sediaan sehingga tidak terbentuk gelembung udara, Sediaan diamati dengan menggunakan pembesaran rendah objektif 10x dan objektif 40x [10].…”