Enzymmuster Und Progesteronsynthese

Brandau, H.; Remmlinger, Karin; Luh, Wilfried

doi:10.1530/acta.0.0560433

Cited by 4 publications

(2 citation statements)

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“…The theca are thought to be the principal steroid-producing compartment in the preovulatory follicle, with the granulosa functioning as steroid secretors only after the corpus luteum has formed (1,4,(14)(15)(16). However, incubated granulosa cells synthesized progesterone throughout the 48-h culture period (Table 4), and well before the cells would have acquired the morphological characteristics of luteinization (1,3).…”

Section: Discussionmentioning

confidence: 99%

Progesterone, Androstenedione, Testosterone, Estrone, and Estradiol Synthesis in Hamster Ovarian Follicle Cells

1975

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Isolated granulosa cells and theca from proestrous hamsters alone and in recombination, were cultured in order to study steroidogenic capacity of this tissue. Cells from medium size antral follicles (100-300 mum diam.) and large preovulatory follicles (500 plus mum diam.) were used. Steroids were measured by radioimmunoassay. Cultures of cells derived from both sizes of follicles made significant amounts of progesterone for up to 6 days in tissue culture. The preparations from the medium sized antral follicles synthesized little or no estrogen. Of the cells harvested from the preovulatory follicles, the granulosa and theca made moderate amounts of estradiol-17beta while the recombined system made similar to 5 times the estradiol-17beta made by theca or granulosa alone. The results indicate that in the in vitro system used: 1) The hamster follicle cells are similar to other species in that they spontaneously luteinize in culture and secrete large amounts of progesterone, 2) Androgen accumulation is greatest in media from cultured theca of preovulatory follicle, 3) A synergism between theca and granulosa of the large preovulatory follicle exists to effect maximal estrogen synthesis, and 4) Estrogen synthesis is short-lived in vitro in contrast to continued progesterone production.

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Section: Discussionmentioning

confidence: 99%

Progesterone, Androstenedione, Testosterone, Estrone, and Estradiol Synthesis in Hamster Ovarian Follicle Cells

1975

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“…Enzyme activities represent an important standard by which meta bolic processes may be analyzed. Investigation has already been carried out into the possibility of a correlation between enzyme activity in the follicle wall and the synthesis of steroids [Deane et cd., 1962;Brandau et al, 1967;Smith and Waynforth, 1970]. Despite extensive knowledge of the presence of enzymes in the ovary tissue, very little is known of the activity of enzymes in the human follicular fluid.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Activity in the Fluid of the Human Ovarian Follicle

Caucig¹,

Friedrich²,

Breitenecker³

et al. 1972

Gynecol Obstet Invest

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By using photometric techniques on standardized test substances, we measured the activity of alkaline and acid phosphatase, glutamic oxaloacetic transaminase, leucine aminopeptidase, aldolase, lactic dehydrogenase (LDH), LDH isoenzyme 1 and malate dehydrogenase in the fluid of human tertiary and Graafian follicles and, by way of comparison, that of ovarian cysts and the serum. All the enzymes, with the exception of alkaline phosphatase, were active to statistically significantly different degrees in the various fluid samples. The activity of the two glycolytic enzymes in particular was statistically significantly higher in the fluid of the tertiary follicle than in any of the other fluids for which measurements were taken.

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