By using photometric techniques on standardized test substances, we measured the activity of alkaline and acid phosphatase, glutamic oxaloacetic transaminase, leucine aminopeptidase, aldolase, lactic dehydrogenase (LDH), LDH isoenzyme 1 and malate dehydrogenase in the fluid of human tertiary and Graafian follicles and, by way of comparison, that of ovarian cysts and the serum. All the enzymes, with the exception of alkaline phosphatase, were active to statistically significantly different degrees in the various fluid samples. The activity of the two glycolytic enzymes in particular was statistically significantly higher in the fluid of the tertiary follicle than in any of the other fluids for which measurements were taken.
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