Enzymic evaluation of thiamin, riboflavin and pyridoxine status of parturient mothers and their newborn infants in a Mediterranean area of Spain

Abstract

Subjects: 131 healthy parturient mothers, with normal pregnancies and deliveries in St Joan University Hospital, and their newborn infants. Interventions: Erythrocyte haemolysates were prepared from maternal blood at delivery and infants' umbilical cord blood and used to measure micronutrient status using the transketolase, glutathione reductase and aspartate aminotransferase coenzyme stimulation tests. Results: Maternal and infant coenzyme activities were signi®cantly correlated, but infant coenzyme status was better than maternal, with signi®cantly higher basal and stimulated activity (P`0.001) and signi®cantly lower activation coef®cients (P`0.001). Inadequate thiamin, ribo¯avin or pyridoxine status occured in 38.2 ± 62.6% (50 ± 82) of the mothers and 3.1 ± 37.4% (4 ± 49) of the infants; 85.2% (46/54), 12.9% (4/31) and 24.1% (12/54) of infants born to mothers with biochemical de®ciency of either thiamin, ribo¯avin or pyridoxine, respectively also had inadequate status. Maternal de®ciencies in more than one vitamin further increased the risk of infant thiamin and pyridoxine de®ciency. Maternal and infant ribo¯avin status were signi®cantly correlated with fetal development (e.g. length at birth, P`0.001). The incidence of thiamin de®ciency in paturient mothers in Spain was the highest out of a 12-country comparison.

Protective association of MTHFR polymorphism on cervical intraepithelial neoplasia is modified by riboflavin status

“…The HC-2 assay (Digene Gaithersburg, MD 20878 USA), selected by the ALTS investigators, detected 13 HR-HPV subtypes (16,18,31,33,35, 39, 45, 51, 52, 56, 58, 59 and 68) and classified patients as positive or negative for HR-HPV. Detailed descriptions of sample collection and methodologies used to assess MTHFR polymorphism and folate, vitamins B-12, A, E, C and total carotene are published elsewhere (10,15).…”

“…Since a marked stimulation occurs with inadequate riboflavin status, a higher AC is indicative of suboptimal riboflavin status. There has been no standard AC value to define suboptimal riboflavin status in different populations; reported values vary from 1.2 to 1.4 (17,18,19).…”

The effects of pyridoxine deficiency and supplementation on hematological profiles, lymphocyte function, and hepatic cytochrome P450 in B6C3F1mice

“…Pyridoxine deficiency decreases the activity of the enzyme with a concomitant induction in the activity coefficient; supplementation of pyridoxine increases the enzyme activity, resulting in a reduction of the activity coefficient (Raica and Sauberlich, 1964;Woodring and Storvick, 1970;Solomon and Hillman, 1978;Kwak et al, 2002). Thus, the measurement of erythrocyte aspartate aminotransferase activity coefficient (EAST-AC) has been frequently used to determine pyridoxine status in both humans and experimental animals (Woodring and Storvick, 1970;Pongpaew et al, 1993;Aramphongphan et al, 1994;Tovar et al, 1996;Sanchez, 1999;Chiang et al, 2005;Oshiro et al, 2005).…”

Association of low intake of milk and vitamin D during pregnancy with decreased birth weight

“…Inadequate consumption of riboflavin is associated with decreases in weight, 9 length and head circumference of babies at birth.…”