Enzymes in Industry

Aehle, Wolfgang

doi:10.1002/9783527617098

Cited by 145 publications

(16 citation statements)

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“…Thus, the enzymatic degradation of lignin is suggested as a better alternative. Lignin peroxidase (LiP) and manganese peroxidase (MnP) are successfully used for biopulping, biobleaching as well as selective delignification in the paper industry and selective delignification helps in the production of cellulosic materials that can be used as efficient feed and biofuel [188,189].…”

Section: Application In Pulp and Paper Industrymentioning

confidence: 99%

A Comprehensive Review on Function and Application of Plant Peroxidases

Pandey¹,

Awasthi²,

Singh³

et al. 2017

Biochem Anal Biochem

259

213

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Peroxidases, one of the key antioxidant enzymes, are widely distributed in nature and catalyze oxidation of various electron donor substrates concomitant with the decomposition of H 2 O 2. The non-animal plant peroxidases (class III peroxidase) are involved in various essential physiological processes of plant growth and development throughout their life cycle. In view of the capability of peroxidases to catalyze the redox reaction for a wide range of substrates, they are considered as one of the important enzyme from the point of view of their various medicinal, biochemical, immunological, biotechnological and industrial applications. They have been successfully used for biopulping and biobleaching in the paper and textile industries. Peroxidases have also been used in organic synthesis, bioremediation, as well as various analytical applications in diagnostic kits, ELISA. Peroxidase based biosensors find application in analytical systems for determination of hydrogen peroxide, glucose, alcohols, glutamate, and choline etc. Thus, in view of array of physiological functions as well as industrial applications, the peroxidases have conquered a dominant position in research groups and become one of the most extensively studied enzymes. In this direction, the present review embodies the classification, mechanism of action, major physiological functions as well as industrial applications of plant peroxidases.

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Section: Application In Pulp and Paper Industrymentioning

confidence: 99%

A Comprehensive Review on Function and Application of Plant Peroxidases

Pandey¹,

Awasthi²,

Singh³

et al. 2017

Biochem Anal Biochem

259

213

View full text Add to dashboard Cite

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“…Other classifications distinguish the presence or absence of carriers/supports (Illanes 2008) or those that differentiate the reversible or irreversible nature of the interactions (Homaei et al 2013). There are also authors that do not classify them, and simply discuss the different methods (Aehle 2007;Moehlenbrock and Minteer 2017;Nguyen, Lee, et al 2019;Sheldon 2007;Sirisha, Jain, and Jain 2016). This latter perspective is that assembling most with the focus of this review.…”

Section: Contribution Of Immobilization Technology For B-galactosidasmentioning

confidence: 99%

“…Entrapment involves the occlusion of an enzyme in a synthetic or natural polymeric network (gel, fiber or microcapsule) that act as a permeable membrane to substrates and products, while the enzyme is retained inside (Guo 2019;Nisha, Karthick, and Gobi 2012;Sheldon 2007). This is a fast, cost-effective and feasible strategy of immobilization but it has some drawbacks, the main one being the mass transfer limitation due to diffusional problems between the substrates, products and the carrier (Aehle 2007;Nisha, Karthick, and Gobi 2012). Additionally, physical retain is generally weak and does not prevent enzyme leakage.…”

Section: Contribution Of Immobilization Technology For B-galactosidasmentioning

confidence: 99%

“…Enzymes are biocatalysts responsible for specific chemical reactions, where a set of reactants (substrates) are converted into specific products during complex metabolic processes essential to sustain life (Illanes, Wilson, and Vera 2014). They are proteins that act as catalysts in living organisms, participating in biological processes, and regulating the rate at which chemical reactions proceed, without altering their equilibrium (Aehle 2007;Purich 2001). Furthermore, enzymes are integral parts of industrial processes since some of the reactions in which they participate would not be feasible without their aid.…”

Section: Introductionmentioning

confidence: 99%

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Recent advances in β-galactosidase and fructosyltransferase immobilization technology

Ureta

Martins

Figueira

et al. 2020

Critical Reviews in Food Science and Nutrition

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The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: b-galactosidases and fructosyltransferases. The main methods used to immobilize these two enzymes are adsorption, entrapment, covalent coupling and cross-linking or aggregation (no support is used), all of them having pros and cons. Regarding the support, it should be cost-effective, assure the reusability and an easy recovery of the enzyme, increasing its stability and durability. The discussion provided showed that the type of enzyme, its origin, its purity, together with the type of immobilization method and the support will affect the performance during the enzymatic synthesis. Enzymes' immobilization involves interdisciplinary knowledge including enzymology, nanotechnology, molecular dynamics, cellular physiology and process design. The increasing availability of facilities has opened a variety of possibilities to define strategies to optimize the activity and re-usability of b-galactosidases and fructosyltransferases, but there is still great place for innovative developments.

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“…Lipase adsorption on solid support is the most convenient, because the procedure is very simple and no modification of enzymes is required [42]. In addition, the surface functionalized adsorbents may be used in downstream processes even for protein separations [39].…”

Section: Novel Enzyme Immobilization Methodsmentioning

confidence: 99%