Enzyme Variations Produced in Epidermis by Soft X-ray Collimated Beams

Klein‐Szanto, Andres J.; Cabrini, R. L.; Itoiz, M. E.; Volco, H.E.

doi:10.1080/09553007414551251

Cited by 4 publications

(3 citation statements)

References 2 publications

(1 reference statement)

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“…These findings are in keeping with those of previous studies which have reported on the increase in the synthesis of enzymes involved in keratinization and activation of the pentose shunt, whose by-products are used in the synthesis of RNA (Boothman and Lee, 1991). Furthermore, it is known that mammalian cells respond to x-irradiation by eliciting a complex series of events which result in the appearance of new genes and proteins (Klein-Szanto et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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The possibility of detection of incipient cellular alterations is central to early diagnosis and to clinician's capacity to discriminate between samples that appear similar on routine preparations. We examined the value of silver-stained nucleolar organizer regions (AgNOR) in detecting radio-induced alterations in a model of squamous epithelium biologically similar to oral mucosa. Morphometry of AgNOR has been proven to be of value in the detection of incipient cellular alterations. This method allows for the quantitative evaluation of lesions induced by high doses of radiation long before they become apparent in routine preparations. We herein examine the capacity of AgNOR to reveal the response to low doses of radiation, closer to the therapeutic or accidental dose to which the epithelium of oral mucosa may be exposed.

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Section: Discussionmentioning

confidence: 99%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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“…The epidermal enzyme activities were determined with a microspectrophotometer provided with an automatic stage (Zeiss Cytoscan) . The methodology of epidermal enzyme activity determinations has been described by Klein-Szanto and Cabrini (1970), Klein-Szanto et al (1974) and Itoiz et al (1974) .…”

Section: Methodsmentioning

confidence: 99%

“…There have also been repeated reports on glycolytic metabolism modifications, showing a constant decrease in enzyme activities related to the Krebs cycle, and an increase in those associated with the pentose shunt (Cabrini, Itoiz, Mayo, Smolko and Klein-Szanto 1970, Kelin-Szanto and Cabrini 1970, Klein-Szanto, Cabrini, Itoiz and Volco 1974 . These enzymatic variations develop to a remarkable extent, as in the case of glucose-6-phosphatase dehydrogenase which, in some irradiation conditions, trebles the activity found in normal epithelia .…”

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confidence: 99%

Variations in the Enzymatic Behaviour of Irradiated Epidermis as Function of Age and Lesion Localization

Itoiz

Frasch

1977

International Journal of Radiation Biology and Related Studies

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Practical applications of the microphotometric quantification of histoenzyme reactions

1981

Histochem J

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There are many histochemical reactions which can be used for the accurate topographic detection of enzymes in tissues. There are also many reports of applications of these techniques in which the results have been evaluated subjectively. However, only a few of these methods have been the subject of quantification, using different methodologies, most of them based on microphotometry. A critical analysis of these procedures has generally shown that they are valid either relatively or at an absolute level. Nevertheless, the routine application of these methods is not as widespread as one might expect. In this paper, the methodological developments that have taken place in the histochemical assay of some enzymes by microspectrophotometry are reviewed briefly. The enzymes include acid phosphatase, non-specific esterase, succinate dehydrogenase and cytochrome oxidase. These methods have given consistent and useful results when applied to our experimental models of irradiated skin, and thus seem useful for various other applications. The reasons why there have been so few applications for quantitative histochemistry are also discussed together with possible ways of making its use more general.

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Enzyme Variations Produced in Epidermis by Soft X-ray Collimated Beams

Cited by 4 publications

References 2 publications

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

Variations in the Enzymatic Behaviour of Irradiated Epidermis as Function of Age and Lesion Localization

Practical applications of the microphotometric quantification of histoenzyme reactions

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