Variations in the Enzymatic Behaviour of Irradiated Epidermis as Function of Age and Lesion Localization

Itoiz, M. E.; Frasch, A. C. C.

doi:10.1080/09553007714550601

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…In particular, this study shows the potential value of AgNOR in the evaluation of the development of post-irradiation mucositis, a relatively frequent lesion of the oral cavity. AgNOR appear to be more sensitive than histological parameters and easier to demonstrate and evaluate than other markers, such as enzyme activity, tested in similar models (Cabrini et al, 1970;Cabrini, 1970, 1972;Itoiz et al, 1974;Itoiz and Frasch, 1977;Schwint et al, 1989). Variations in AgNOR are not specific to a particular lesion but reveal, with great sensitivity, changes in cellular activity associated with different entities.…”

Section: Discussionmentioning

confidence: 97%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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The possibility of detection of incipient cellular alterations is central to early diagnosis and to clinician's capacity to discriminate between samples that appear similar on routine preparations. We examined the value of silver-stained nucleolar organizer regions (AgNOR) in detecting radio-induced alterations in a model of squamous epithelium biologically similar to oral mucosa. Morphometry of AgNOR has been proven to be of value in the detection of incipient cellular alterations. This method allows for the quantitative evaluation of lesions induced by high doses of radiation long before they become apparent in routine preparations. We herein examine the capacity of AgNOR to reveal the response to low doses of radiation, closer to the therapeutic or accidental dose to which the epithelium of oral mucosa may be exposed.

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Section: Discussionmentioning

confidence: 97%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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Practical applications of the microphotometric quantification of histoenzyme reactions

1981

Histochem J

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There are many histochemical reactions which can be used for the accurate topographic detection of enzymes in tissues. There are also many reports of applications of these techniques in which the results have been evaluated subjectively. However, only a few of these methods have been the subject of quantification, using different methodologies, most of them based on microphotometry. A critical analysis of these procedures has generally shown that they are valid either relatively or at an absolute level. Nevertheless, the routine application of these methods is not as widespread as one might expect. In this paper, the methodological developments that have taken place in the histochemical assay of some enzymes by microspectrophotometry are reviewed briefly. The enzymes include acid phosphatase, non-specific esterase, succinate dehydrogenase and cytochrome oxidase. These methods have given consistent and useful results when applied to our experimental models of irradiated skin, and thus seem useful for various other applications. The reasons why there have been so few applications for quantitative histochemistry are also discussed together with possible ways of making its use more general.

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Variations in epidermal cytochrome oxidase activity after local irradiation

1982

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Cytochrome oxidase activity was evaluated histochemically as an index of mitochondrial damage after local irradiation with X-rays. It was determined by microphotometry on the tail skin of newly born Wistar rats four days after irradiation with doses ranging from 2 to 16 krad. The enzyme activity of the whole epidermis increased after irradiation, the increases being related to the increase in thickness of the epithelium which was observed as a response to irradiation injury. Within the dose range tested, the enzyme concentration (expressed per uni volume of tissue) decreased in relation to the dose applied. At the electron microscopy level, the cytochemical demonstration of cytochrome oxidase revealed an irregular reaction over the cristae, intramitochondrial vacuolization and partial homogenization of the matrix. Positive membrane fragments were seen around lipid droplets. This reaction confirms the mitochondrial origin of these previously observed radiation-induced vacuoles.

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Variations in the Enzymatic Behaviour of Irradiated Epidermis as Function of Age and Lesion Localization

Cited by 4 publications

References 7 publications

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

Practical applications of the microphotometric quantification of histoenzyme reactions

Variations in epidermal cytochrome oxidase activity after local irradiation

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