AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

lelmini, M.V.; Heber, Elisa M.; Schwint, Amanda E.; Cabrini, R. L.; Itoiz, M. E.

doi:10.1177/00220345000790031101

Cited by 10 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because the criteria for AgNOR count in histological sections differ from the criteria for cytological smears. 20 Although, many studies have performed AgNOR count in oral mucosa with benign, premalignant, and malignant tumors (a few of which were found in those exposed to tobacco smoking and alcohol), we didnot find any study that has assessed normal oral mucosa exposed to peppers or hot food and drink consumption. Most previous studies that have assessed oral mucosal lesions by AgNOR focused solely on the AgNOR count, and very few studies have added keratinization of the oral mucosal cells besides AgNOR counts.…”

Section: Discussionmentioning

confidence: 90%

Oral epithelial atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, peppers and hot meals, using the AgNOR and Papanicolaou staining techniques

Ahmed

Ebnoof

Hussein

et al. 2009

Diagnostic Cytopathology

View full text Add to dashboard Cite

To evaluate cytological atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, hot meals, and peppers using the AgNOR and Papanicolaou methods. A total of 180 individuals were evaluated, of which 60 were smokers, 34 were alcohol users, 52 were habitual peppers and hot meal (exposed) consumers, 24 were non-exposed, and 10 were patients with Oral Squamous Cell Carcinoma (OSCC), as an internal control. Cytological materials were obtained by brushing of buccal mucosa, on the border of the tongue and on the floor of the mouth, and participants underwent the Papanicolaou test for cytological changes and AgNOR staining for evaluation of the mean number of AgNOR dots per nucleus. SPSS program was used to perform the Pearson chi-square test. The 95% confidence level, Odds Ratio (OR), and the 95% Confidence Intervals (CI) were used. The features of cytological atypia were verified among 10 individuals, including 5 smokers, 2 alcohol users, 2 hot meals and peppers consumers, and one non-exposed. For atypia among tobacco smokers, the adjusted Odds Ratio (OR) and the 95% CI were found to be 2 (0.246-16.24). Increased keratinization was detected among 27 (45%) of the smokers (P < 0.0001), 17 (32.7%) of the pepper and hot meals consumers (P < 0.005), 4 (11.8%) of the alcohol consumers, and among 2 (3.7%) of the non-exposed group. Statistical analyses revealed a greater mean number of AgNORs per nucleus in smokers (3.68) followed by (2.82) alcohol consumers, compared to the habitual peppers and hot meal consumers (2.28) and the non-exposed group (2.00). What's more, 80% of the smears with cytological atypia were identified with 6 +/- 2 AgNOR mean count. The increase of the variables suggests that the evaluation of epithelial atypical changes in individuals exposed to smoking and alcohol carcinogens may be a useful screening tool. While hot meals and peppers did not seem to be a risk for oral mucosal proliferation, they increased the potency of keratinization and infection.

show abstract

Section: Discussionmentioning

confidence: 90%

Oral epithelial atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, peppers and hot meals, using the AgNOR and Papanicolaou staining techniques

Ahmed

Ebnoof

Hussein

et al. 2009

Diagnostic Cytopathology

View full text Add to dashboard Cite

show abstract

“…Silver stainable nucleolar organizer regions (AgNORs) have been shown to be replicatory markers (5). Studies suggest potential usefulness of AgNOR parameters, such as mean count and area of distribution within the nucleus, in diagnosis and prognosis of various neoplasms (5–11), especially oral squamous cell carcinoma (6, 9, 12, 13). Mean AgNOR counts can be useful in diagnosing histological features representing oral leukoplakia (6) and have been shown to correlate with clinical outcomes of various cancers (12, 14–16).…”

mentioning

confidence: 99%

Usefulness of AgNOR counts in diagnosing epithelial dysplasia

Ray

Chattopadhyay

Caplan

2003

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…Silver stainable nucleolar organizer regions (AgNORs) have been shown to be replicatory markers and many studies have shown the potential usefulness of AgNOR parameters like mean count area of distribution in the nucleus in diagnosis of various neoplasms (5–10). Oral squamous cell carcinoma can be distinguished from normal epithelium by AgNOR count (5, 8, 11, 12).…”

mentioning

confidence: 99%