AgNOR count as objective marker for dysplastic features in oral leukoplakia

Chattopadhyay, Amit; Ray, Jharna; Caplan, Daniel J.

doi:10.1034/j.1600-0714.2002.00153.x

Cited by 24 publications

(24 citation statements)

References 16 publications

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“…The mean AgNOR count increased as the grade of dysplasia increased in cases of OL, where as in the cases of OSCC there was no stastically significant difference between well and moderately differentiated OSCC. The AgNOR count has been thought to be a proliferation marker; therefore one would find a difference in normal mucosa, OL and OSCC as reported in literature (12)(13)(14)(15)(16). The inter observer variation between the two observers in our study was highly statistically significant (p<0.0001) indicating that the AgNOR counting method employed in our study is reliable.…”

Section: Comparison Of Agnor Count In Different Grades Of Ol and In Osupporting

confidence: 67%

“…In one study the cutoff value of 4.8 was proposed to differentiate between benign and malignant lesions in brush biopsy cases of suspicious lesions of oral cavity (17). In another study a cutoff point of 2.37 was proposed to differentiate between nondysplastic and dysplastic OL (16). Another study proposed that a mean AgNOR count >2.8 concurred with poor prognosis in OSCC and that T3 and T4 tumors >2.8 mean AgNOR counts were aggressive and may exhibit resistance to current treatment protocols (12).…”

Section: Comparison Of Agnor Count In Different Grades Of Ol and In Omentioning

confidence: 99%

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Analysis of cell proliferation rate in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Mehkri¹,

Iyengar²,

Nagesh³

et al. 2010

J Clin Exp Dent

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Objectives: Assessment of the cell proliferation rate in tissues can be one of the markers for impending malignancy in precancers. The state of activation and the proliferation activity of the cells can be assessed by the frequency of silver stained Nucleolar Organiser regions (AgNOR) within the nuclei which is significantly higher in malignant cells. The present study was carried out to analyze the distribution of the AgNOR in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC), and in their various histological grades, and to assess if the AgNOR distribution could give information on the malignant potentiality in premalignant lesions and aggressiveness of the malignant lesions. Study design: The study specimens comprised of 35 archival cases, of which 15 cases were of OL and 20 cases of OSCC. The specimens were stained by hematoxylin and eosin and modified silver staining method of Ploton et al. for the Nucleolar Organiser Regions. The specimens were analyzed independently by the two observers and was further statistically analysed. Results: The mean AgNOR count in OL was 2.80 ±0.50 and in cases of OSCC was 5.71± 1.08. The mean AgNOR count in OL cases of mild dysplasia was 2.59 ±0.66, in moderate dysplasia was 2.92± 0.43 and in severe dysplasia was 2.79. The mean AgNOR count in cases of well differentiated OSCC was 5.73± 1.62 and in cases of moderately differentiated OSCC was 5.67±1.19. Conclusion: The mean AgNOR count was higher in cases of OSCC as compared to cases of OL, and the AgNOR counts increased with the increase in the grades of dysplasia indicating a higher proliferative rate with increase in dysplasia.

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Section: Comparison Of Agnor Count In Different Grades Of Ol and In Osupporting

confidence: 67%

Section: Comparison Of Agnor Count In Different Grades Of Ol and In Omentioning

confidence: 99%

Analysis of cell proliferation rate in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Mehkri¹,

Iyengar²,

Nagesh³

et al. 2010

J Clin Exp Dent

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“…Thus, the larger the number of NORs, the higher the replication rate of ribosomes and cells. This technique has therefore been used for the quantification of cell proliferation in different tissues and lesions [11,13,17].…”

Section: Introductionmentioning

confidence: 99%

“…Considering the possible influence of smoking on the occurrence of oral cancer and of precursor lesions, together with the accessible location of the lesions which permits easy visualization and collection of material for analysis, exfoliative cytology might be a useful tool for the detection and monitoring of initial alterations [9,10] and for the establishment of adequate treatment in smokers [3,[11][12][13][14][15][16]. It is a complementary diagnostic method which presents several advantages such as rapid and easy execution, low cost, diagnostic safety, efficacy and noninvasiveness, and can be repeated several times [9,12,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Exfoliative Pap Stain and AgNOR Counts of the Tongue in Smokers and Nonsmokers

Fontes

Corrêa

Issa

et al. 2008

Head and Neck Pathol

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“…therefore, sensitive and specific biomarkers for oscc may be helpful in screening high-risk olK patients. Attempts to find biomarkers that identify premalignant oscc and cancerous lesions have resulted in several candidate genes associated with oscc tumor progression including p53, cyclin D1, epidermal growth factor receptor (13,14), Agnor (15), and Ki-67 (16). However, to date there are no widely accepted molecular biomarkers for olK carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

Identification of genes related to carcinogenesis of oral leukoplakia by oligo cancer microarray analysis

Liu

Wen-ling²,

Xie³

et al. 2011

Oncol Rep

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Abstract. the aim of this study was to identify genes that are predictive of carcinogenic change in patients of oral leukoplakia (olK) using a cancer-related microarray. candidate biomarkers were discovered using the oligo geArray oHs-802 and validated on independent samples by semi-quantitative reverse transcription-pcr (rt-pcr) and real-time rt-pcr. Both the discovery and the validation cohorts of samples included normal oral tissues, olK tissues and oral squamous cell carcinoma (oscc) tissues. Based on the microarray results, we found that there were nine genes successively up-regulated or down-regulated more than 2-fold between the normal group and olK group and then again between the olK group and oscc group. the expression levels of the nine signature genes had statistically significant differences (p<0.05) between the olK and normal groups and between the oscc and olK groups. In summary, the expression of the 9 signature genes might be representative of olK carcinogenesis. A cancer-related microarray was used to identify a panel of candidate biomarkers for determining carcinogenesis of olK lesions, in combination with semi-quantitative and real-time rt-pcr to validate the results. our data indicated that alterations in gene expression that result in carcinogenesis can be identified in precancerous oral tissues.

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AgNOR count as objective marker for dysplastic features in oral leukoplakia

Abstract: Mean AgNOR count could be a valuable criterion for defining objective parameters for diagnosis/determination of dysplasia distinguishing between dysplastic and non-dysplastic leukoplakia.

Cited by 24 publications

References 16 publications

Analysis of cell proliferation rate in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Analysis of cell proliferation rate in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Comparison of Exfoliative Pap Stain and AgNOR Counts of the Tongue in Smokers and Nonsmokers

Identification of genes related to carcinogenesis of oral leukoplakia by oligo cancer microarray analysis

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