Enzyme Shielding in an Enzyme‐thin and Soft Organosilica Layer

Correro, M. Rita; Moridi, Negar; Schützinger, Hansjörg; Sykora, Sabine; Ammann, Erik M.; Peters, Erich Henrik; Dudal, Yves; Corvini, Philippe F.-X.; Shahgaldian, Patrick

doi:10.1002/ange.201600590

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…Concomitantly, AP activity dropped from 90±1.2 U g −1 (U per gram of SNPs) to 11±0.2 U g −1 . This loss in activity was consistent with our previous results, where we demonstrated that a curing phase is necessary to recover enzymatic activity through reorganization of the protective organosilica layer . Accordingly, the particles were incubated at 25 °C for 24 h for the curing reaction.…”

Section: Figuresupporting

confidence: 92%

A Biocatalytic Nanomaterial for the Label‐Free Detection of Virus‐Like Particles

et al. 2017

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The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.

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Section: Figuresupporting

confidence: 92%

A Biocatalytic Nanomaterial for the Label‐Free Detection of Virus‐Like Particles

et al. 2017

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“…Following the immobilization of AuNR–IgG bioconjugates on glass substrates, a polymer encapsulation layer is formed through copolymerization of (3-aminopropyl) trimethoxysilane (APTMS) and trimethoxy(propyl)silane (TMPS) on AuNR and around immobilized IgG. The methoxy group of TMPS and APTMS undergoes rapid hydrolysis to form methanol and trisilanols. , Hydrolysis is followed by condensation of the silanols, which results in the formation of an amorphous aminopropyl functional polymer layer consisting of Si–O–Si bonds and functional end groups such as hydroxyl (−OH), amine (−NH 3 + ), and methyl (−CH 3 ). , These end groups interact noncovalently via hydrogen bonding, hydrophobic, and electrostatic interactions with AuNR–IgG bioconjugates resulting in the formation of a stable organosilica layer around them. Next, bifunctional PEG (methoxy-PEG-silane) was covalently grafted on to the free regions of the organosilica layer.…”

Section: Resultsmentioning

confidence: 99%

Refreshable Nanobiosensor Based on Organosilica Encapsulation of Biorecognition Elements

Gupta

Luan

Chakrabartty

et al. 2020

ACS Appl. Mater. Interfaces

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Implantable and wearable biosensors that enable monitoring of biophysical and biochemical parameters over long durations are highly attractive for early and presymptomatic diagnosis of pathological conditions and timely clinical intervention. Poor stability of antibodies used as biorecognition elements and the lack of effective methods to refresh the biosensors upon demand without severely compromising the functionality of the biosensor remain significant challenges in realizing protein biosensors for long-term monitoring. Here, we introduce a novel method involving organosilica encapsulation of antibodies for preserving their biorecognition capability under harsh conditions, typically encountered during the sensor refreshing process, and elevated

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“…To achieve a thin polymer encapsulation, 0.4 μL of TMPS and 0.4 μL of APTMS were added to 1 mL of the AuNR–PA–IgG bioconjugate solution under gentle stirring at room temperature for 30 min and then left overnight at 4 °C. , The thickness of the encapsulation layer can be controlled by varying the volume of TMPS and APTMS added to the bioconjugate solution and can be monitored via LSPR wavelength shifts measured with a ultraviolet–visible (UV–vis) spectrophotometer. After that, the solution was centrifuged at 8000 rpm for 10 min to remove free polymers and then resuspended in 1× PBS for further usage.…”

Section: Methodsmentioning

confidence: 99%

“…This not only restricts the printability of these bioinks without compromising their functionality but also leads to limited stability and short shelf life of the printed biosensors. Most recent progress in preserving the biofunctionality of biosensor chips involves coating the entire chips with protective materials, such as metal–organic frameworks and organosiloxane. − However, these approaches are limited to immobilized bionanoconjugates on rigid substrates and not compatible with printing processes. Realization of ultrastable bionanoconjugate inks that are resistant to harsh conditions remains challenging.…”

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confidence: 99%

Ultrastable Plasmonic Bioink for Printable Point-Of-Care Biosensors

Yin

Guo

et al. 2020

ACS Appl. Mater. Interfaces

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Point-of-care biosensors are critically important for early disease diagnosis and timely clinical intervention in resource-limited settings. The real-world application of these biosensors requires the use of stable biological reagents and cost-effective fabrication approaches. To meet these stringent requirements, we introduce a generic encapsulation strategy to realize ultrastable plasmonic bioink by encapsulating antibodies with an organosiloxane polymer through in situ polymerization. Plasmonic nanostructures serve as sensitive nanotransducers, allowing for label-free biochemical detection. The plasmonic bioink with encapsulated antibodies exhibits excellent thermal, biological, and colloidal stabilities making it compatible with printing process. As a proof-of-concept, we demonstrate the printability of the ultrastable plasmonic bioinks on different types of substrates with direct writing techniques. The organosiloxane polymer preserves the biorecognition capabilities of the biosensors under harsh conditions, including elevated temperature, exposure to chemical/biological denaturants, and ultrasonic agitation. Plasmonic biochips fabricated with the ultrastable ink exhibit superior stability compared to the biochips with unencapsulated antibodies.

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Enzyme Shielding in an Enzyme‐thin and Soft Organosilica Layer

Cited by 7 publications

References 23 publications

A Biocatalytic Nanomaterial for the Label‐Free Detection of Virus‐Like Particles

A Biocatalytic Nanomaterial for the Label‐Free Detection of Virus‐Like Particles

Refreshable Nanobiosensor Based on Organosilica Encapsulation of Biorecognition Elements

Ultrastable Plasmonic Bioink for Printable Point-Of-Care Biosensors

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