Ultrastable Plasmonic Bioink for Printable Point-Of-Care Biosensors

Abstract

Point-of-care biosensors are critically important for early disease diagnosis and timely clinical intervention in resource-limited settings. The real-world application of these biosensors requires the use of stable biological reagents and cost-effective fabrication approaches. To meet these stringent requirements, we introduce a generic encapsulation strategy to realize ultrastable plasmonic bioink by encapsulating antibodies with an organosiloxane polymer through in situ polymerization. Plasmonic nanostructures serve as sensitive nanotransducers, allowing for label-free biochemical detection. The plasmonic bioink with encapsulated antibodies exhibits excellent thermal, biological, and colloidal stabilities making it compatible with printing process. As a proof-of-concept, we demonstrate the printability of the ultrastable plasmonic bioinks on different types of substrates with direct writing techniques. The organosiloxane polymer preserves the biorecognition capabilities of the biosensors under harsh conditions, including elevated temperature, exposure to chemical/biological denaturants, and ultrasonic agitation. Plasmonic biochips fabricated with the ultrastable ink exhibit superior stability compared to the biochips with unencapsulated antibodies.

Bi2S3@MoS2 Nanoflowers on Cellulose Fibers Combined with Octahedral CeO2 for Dual-Mode Microfluidic Paper-Based MiRNA-141 Sensors

“…Recent years, paper is used widely as an interesting biosensor matrix owing to its high porosity, excellent flexibility, and easy-to-functionalize characteristics, , which provides new possibilities for cost-effective point-of-care testing, , especially at resource-scarce and remote areas. Furthermore, the microfluidic paper-based analysis device (μPAD) using origami technology can realize the functionalization of different areas with the help of inkjet printing technology, thereby allowing the fluid to flow through capillary action for multiple analysis. − Recently, the integration of different analytical techniques including colorimetry, photoelectrochemistry, and electrochemistry with μPAD has achieved the detection of various targets. , Particularly, with the paper-based ingenious design, the electrochemical/visual biological dual-mode sensing platform is explored as the most promising analysis strategy to achieve fast prediction and sensitive detection.…”

Detection of the Chilli Leaf Curl Virus Using an Attenuated Total Reflection-Mediated Localized Surface-Plasmon-Resonance-Based Optical Platform

“…Therefore, the LOD of GaHIgG for this sensor was found to be 0.5 μg/mL from this antibody–antigen study. Recently, the reported LOD of IgG in the PBS buffer was demonstrated between 0.1 to 0.2 μg/mL. , …”

“…Recently, the reported LOD of IgG in the PBS buffer was demonstrated between 0.1 to 0.2 μg/mL. 90,95 Detection of the Plant Virus (ChiLCV). After a successful demonstration of the ATR-LSPR platform as a potential biosensor, it was further employed to detect the plant virus, where ChiLCV DNA was chosen as the target molecule.…”

“…Both absorbances were found to be linearly proportional to the concentration of GaHIgG, which can explain the transport kinetics of molecular interactions. 94,95 The change in A t sometimes may give false results during nanoparticle immobilization, as well as when receptor binding was improper or any surface damage occurred during experimental procedures. However, the calculation of ΔA b by subtracting the buffer line after recording the absorbance response even for a lower concentration of target analytes precisely confirmed the binding and thus ensured the detection of target molecules.…”

Plasmonic stop-band filter based on an MIM waveguide coupled with cavity resonators

“…Due to the confinement of the SPPs energy to a small region along the interface, a sub-wavelength-size optical elements can be realized [1,2,3]. Because of their properties, such as high speed and sensitivity, the SPPs find numerous applications, the most promising of which are in photonic circuits [4] and biological sensing [5,6].…”